Recombinant human TPO protein - Bioactive cytokine

Recombinant cytokine, source: CHO cells

ABOUT

Human TPO protein - Mammalian cell-expressed, tag-free, carrier-free

Recombinant human TPO is a high-quality and biologically active cytokine, validated using proprietary TPO reporter cells. This hematopoietic growth factor is produced in CHO cells to ensure protein glycosylation and bona fide 3D structure.

Recombinant human TPO can be used together with HEK-Blue™ TPO cells for screening molecules that activate or inhibit the TPO pathway, such as receptor agonists or monoclonal antibodies, respectively (see figures).
 

Key features

  • Each lot is validated using HEK-Blue™ TPO cells
  • Endotoxin ≤ 0.1 EU/µg
  • 0.22 µm sterile-filtered

Applications

  • Standard for TPO detection and quantification assays
  • Screening and release assays for antibodies blocking TPO signaling
  • Screening and release assays for engineered TPO

 

TPO is a key hematopoietic growth factor that plays a vital role in the regulation of megakaryocytopoiesis and the maintenance of hematopoietic stem cells (HSCs).

More details

All InvivoGen products are for internal research use only, and not for human or veterinary use.

TPO signaling and biological functions
TPO signaling and biological functions

SPECIFICATIONS

Specifications

Source
CHO cells
Species
Human
Synonyms
C-mpl ligand (ML)
Megakaryocyte colony-stimulating factor
Megakaryocyte growth and development factor (MGDF)
Myeloproliferative leukemia virus oncogene ligand
Accession sequence

P40225

Protein size
174 a.a. (S22-L195)
Molecular weight
~ 23 kDa (SDS-PAGE)
Carrier
Carrier-free
Tag
Tag-free
Purity
≥ 95% (SDS-PAGE)
Solubility

100 μg/ml in water

Formulation buffer

Phosphate buffer saline (pH 7.0), 2.5% saccharose

Appearance (form)
Lyophilized
Reconstitution buffer
Endotoxin-free water (provided)
Sterility

0.22 µm filtration

Endotoxin

The absence of bacterial contamination (e.g. lipoproteins and endotoxins) has been confirmed using HEK-Blue™ TLR2 and HEK‑Blue™ TLR4 cells.

Applications

Cellular assays (tested), ELISA

Quality control

Each lot is functionally tested and validated.

CONTENTS

Contents

  • Product: 
    Recombinant human TPO
  • Cat code: 
    rcyc-htpo
  • Quantity: 
    10 µg
Includes:

1.5 ml endotoxin-free water

Shipping & Storage

  • Shipping method:  Room temperature

    • Storage:

    • -20°C
    Stability: -20°C for up to 1 year

    Caution:

    • Avoid repeated freeze-thaw cycles

Details

TPO background

The hematopoietic growth factor thrombopoietin (TPO) plays a vital role in the regulation of megakaryocytopoiesis and the maintenance of hematopoietic stem cells (HSCs) [1]. TPO promotes the proliferation and differentiation of megakaryocytes and their specialized progenitors. Additionally, TPO drives platelet production and activity. The number of circulating platelets must be tightly controlled to avoid spontaneous bleeding or arterial occlusion and organ damage. TPO binding to the thrombopoietin receptor (TPO-R; also known as c-MPL) activates Janus kinase 2 (JAK2) and signal transducer and activator of transcription 5 (STAT5), initiating the expression of TPO-inducible genes [2].
 

Relevance for therapeutics development

Abnormal levels of TPO or mutations in its receptor are associated with blood disorders, such as thrombocytosis and thrombocytopenia. Of note, TPO-R agonists have been successfully used to treat thrombocytopenia [3].

 

​References:

1. Grozovsky R. et al., 2015. Novel mechanisms of platelet clearance and thrombopoietin regulation. Curr Opin Hematol 22(5):445-51.
2. Hitchcock I., & Kaushansky K., 2014. Thrombopoietin from beginning to end. Br J Haematol. 165(2):259-68.
3. Ghanima W. et al., 2019. Thrombopoietin receptor agonists: ten years later. Haematologica. 104(6):1112-23.

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