Anti-hIFNAR-hIgG1
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Cat.code:
hifnar-mab1
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ABOUT
IFNAR Antagonist - Human IgG1 (high effector functions)
Anti-hIFNAR-hIgG1 is an anifrolumab-based research antibody. Anifrolumab (MDX-1333, MEDI-546) is a fully human IgG1κ monoclonal antibody containing a triple mutation (L234F/L235E/P331S) to reduce Fc effector functions. It binds the human type I interferon receptor subunit 1 (IFNAR1), thereby inhibiting type I interferon signaling. Anifrolumab is FDA-approved for the treatment of adults with moderate to severe systemic lupus erythematosus (SLE).
Anti-hIFNAR-hIgG1 comprises the variable region of Anifrolumab fused to a wild-type human IgG1 constant region, preserving Fc-mediated effector functions. For applications requiring minimal Fc effector activity, InvivoGen also offer Anti-hIFNAR-hIgG1-FES, an effectorless biosimilar containing the triple Fc mutation of Anifrolumab.
This antibody is suitable for effector function analysis, screening assays, and neutralization assays. It can be used together with HEK-Blue™ IFN-α/β cells to inhibit recombinant human IFN-α- or IFN-β-induced type I interferon signaling (see figure).
Key features
- Each lot is functionally tested and validated
- The complete sequence of the antibody construct has been verified
- Absence of endotoxins determined by the EndotoxDetect assay
All products are for research use only, and not for human or veterinary use.
InvivoGen also offers:
SPECIFICATIONS
Specifications
IFNAR1
Human
Neutralization assay, ELISA, Effector function analysis
Sodium phosphate buffer with glycine, saccharose, and stabilizing agents
Neutralization assay
Each lot is functionally tested and validated.
CONTENTS
Contents
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Product:Anti-hIFNAR-hIgG1
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Cat code:hifnar-mab1
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Quantity:100 µg
Shipping & Storage
- Shipping method: Room temperature
- -20°C
- Avoid repeated freeze-thaw cycles
Storage:
Caution:
Details
Anifrolumab background
Anifrolumab is a fully human mAb designed to target the type I interferon (IFN) receptor subunit 1 (IFNAR1) and to block the type I IFN signaling. Anifrolumab features a triple mutation L234F/L235E/P331S in the heavy chain to reduce engagement with the cell surface Fc gamma receptor (FcγR) and potential Fc-mediated effector function, such as antibody-dependent cell-mediated cytotoxicity (ADCC) [1-2]. According to the FDA, it is a first-in-class medication highlighting its innovative and unique mechanism of action [3]. It has been approved as an add-on therapy for moderately to severely active systemic lupus erythematosus (SLE) [3].
References:
1. Plüß M, et al., 2022. Rapid Response of Refractory Systemic Lupus Erythematosus Skin Manifestations to Anifrolumab-A Case-Based Review of Clinical Trial Data Suggesting a Domain-Based Therapeutic Approach. J Clin Med. 11(12):3449.
2. Riggs JM, et al., 2018. Characterization of anifrolumab, a fully human anti-interferon receptor antagonist antibody for the treatment of systemic lupus erythematosus. Lupus Sci Med. 5(1):e000261.
3. U.S. Food and Drug Administration (FDA) (Report). 2022. Advancing Health Through Innovation: New Drug Therapy Approvals 2021.
DOCUMENTS
Documents
Technical Data Sheet
Validation Data Sheet
Safety Data Sheet
Certificate of analysis
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