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Human SAMHD1-KO Dual Reporter THP-1 Cells

NF-κB-SEAP & IRF-Lucia reporter monocytes

Validation of SAMHD1 knockout

Functional validation of SAMHD1 knockout (NF-κB response)

Functional validation of SAMHD1 knockout (IRF response)

Human DNase2-KO Dual Reporter THP-1 Cells

NF-κB-SEAP & IRF-Lucia reporter monocytes

Validation of DNASE2 knockout

Functional validation of DNASE2 knockout (NF-κB response)

Functional validation of DNASE2 knockout (IRF response)

Human IRF7-KO Dual Reporter THP-1 Cells

NF-κB-SEAP & IRF-Lucia reporter monocytes

Validation of IRF7 KO

Functional validation of IRF7 knockout (NF-κB response)

Functional validation of IRF7 knockout (IRF response)

Human IRF5-KO Dual Reporter THP-1 Cells

NF-κB-SEAP & IRF-Lucia reporter monocytes

Validation of IRF5 KO

Functional validation of IRF5 knockout (NF-κB response)

Functional validation of IRF5 knockout (IRF response)

Human IRF1-KO Dual Reporter THP-1 Cells

NF-κB-SEAP & IRF-Lucia reporter monocytes

Validation of IRF1 KO

Functional validation of IRF1 knockout (NF-κB response)

Functional validation of IRF1 knockout (IRF response)

Human RIG-I-KO Dual Reporter THP-1 Cells

NF-κB-SEAP & IRF-Lucia reporter monocytes

Validation of RIG-I knockout

Functional validation of RIG-I knockout (IRF response)

Functional validation of RIG-I knockout (NF-κB response)

Mouse ASC-expressing and Gasdermin D-KO RAW 264.7 Cells

ASC-OE & GSDMD-KO mouse macrophages

Validation of GSDMD KO in RAW-ASC KO-GSDMD cells

Absence of mature IL-1β by KO-GSDMD cells upon canonical inflammasome activation

Absence of mature IL-1β secretion in KO cells upon non-canonical inflammasome activation

Mouse ASC-expressing RAW 264.7 Cells

ASC-OE mouse macrophages

Validation of ASC expression in RAW-ASC cells

Secretion of mature IL-1β by RAW-ASC cells upon inflammasome activation

Human Caspase 4-KO THP-1 Cells

CASP4-KO monocytes

Validation of CASP4 KO in THP1-KO-CASP4 cells

Secretion of mature IL-1β by THP1-KO-CASP4 cells

Human ASC-KO THP-1 Cells

ASC-KO monocytes

Validation of ASC KO in THP1-KO-ASC cells

Absence of mature IL-1β secretion by THP1-KO-ASC cells

Absence of pyroptosis in THP1-KO-ASC cells

Human NLRP3-KO THP-1 Cells

NLRP3-KO monocytes

Validation of NLRP3 KO in THP1-KO-NLRP3 cells

Absence of mature IL-1β secretion in THP1-KO-NLRP3 cells

Absence of pyroptosis in THP1-KO-NLRP3 cells (LDH read-out)

Mouse IRF5-KO Reporter RAW 264.7 Cells

IRF-Lucia reporter mouse macrophages

Validation of IRF5 knockout by PCR

Validation of IRF5 knockout by Western blot (Wes™)

DNA- or RNA-induced IRF response in RAW-Lucia™ ISG-derived cells

Type I interferon-or LPS-induced IRF response in RAW-Lucia™ ISG-derived cells

Mouse STING-KI Dual Reporter THP-1 Cells

NF-κB-SEAP & IRF-Lucia reporter monocytes

Validation of mSTING knockin by PCR

Validation of mSTING knockin by Western blot (WES by Protein Simple)

IRF activation (Lucia luciferase reporter)

NF-kB activation (SEAP reporter)

Human IRF3-KO Dual Reporter THP-1 Cells

NF-κB-SEAP & IRF-Lucia reporter monocytes

Validation of IRF3 knockout by PCR

Validation of IRF3 knockout by Western blot (Wes™)

2'3'-cGAMP-induced IRF and NF-κB responses in THP1-Dual™-derived cells

RNA-induced IRF and NF-κB responses in THP1-Dual™-derived cells

Cytokine or LPS-induced IRF and NF-κB responses in THP1-Dual™-derived cells

Human TBK1-KO Dual Reporter THP-1 Cells

NF-κB-SEAP & IRF-Lucia reporter monocytes

Validation of TBK1 knockout by Western blot (Wes™)

2’3’-cGAMP-induced IRF and NF-κB responses in THP1-Dual-derived cells

RNA-induced IRF and NF-κB responses in THP1-Dual-derived cells

Cytokine or LPS-induced IRF and NF-κB responses in THP1-Dual-derived cells

Mouse IRF1-KO Reporter RAW 264.7 Cells

IRF-Lucia reporter mouse macrophages

Validation of IRF1 knockout by PCR

Validation of IRF1 knockout by Western blot

IRF response in RAW-Lucia™ ISG-derived cells

Human TLR4-KO Dual Reporter THP-1 Cells

NF-κB-SEAP and IRF-Lucia reporter monocytes

PCR validation of TLR4 KO

NF-κB responses in THP1-Dual™ KO-TLR4 cells

IRF responses in PMA-differentiated THP1-Dual™ KO-TLR4 cells

Human TLR2-KO Dual Reporter THP-1 Cells

NF-κB-SEAP and IRF-Lucia reporter monocytes

Validation of TLR2 KO

NF-κB responses in THP1-Dual™ KO-TLR2 cells

IRF responses in THP1-Dual™ KO-TLR2 cells

Human TLR9 Dual Reporter THP-1 Cells

NF-κB-SEAP and IRF-Lucia reporter monocytes

Validation of TLR9 overexpression

Validation of the NF-κB and IRF reporter systems in THP1-Dual™ hTLR9 cells

NF-κB and IRF responses to TLR9 agonists in THP1-Dual™ hTLR9 cells

Human LC3B Reporter THP-1 Cells

RFP::GFP::LC3-OE reporter monocytes

Detection of autophagic fluorescent puncta