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Lucia®-tagged Fc-fusion protein expression plasmid - Mouse IgG2a
Production of Lucia luciferase-tagged IgG2a-Fc fusion proteins
pFUSEN-Lucia-mG2aFc is a cloning plasmid for the generation of a Lucia luciferase-tagged Fc-fusion protein expressing the Fc region (CH2 and CH3 domains) of the murine IgG2a heavy chain and the hinge region fused to the N-terminus of a protein of interest.
Lucia is a secreted luciferase reporter protein with advantageous characteristics when associated with Fc-fusion proteins. It possesses superior carrier ability for excellent secretion of the chimeric protein. It provides a simple means to screen for recombinant clones and it minimally affects the activity of the protein of interest.
Mouse IgG2a-Fc is the murine equivalent of human IgG1-Fc. It has moderate to high affinity for FcγRs and complement receptors, inducing strong ADCC and CDC.
Isotype : IgG2A mouse
Effector Activities : ADCC+++, CDC +++
Selectable with Zeocin™ in E. coli and mammalian cells.
For transient or stable transfection.
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• 20 μg of lyophilized DNA
• 4 pouches of E. coli Fast-Media® Zeo (2 TB and 2 Agar)
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