pFUSE-hIgG3-Fc

Fc-fusion protein expression plasmid - Human IgG3

ABOUT

Fc-fusion protein expression plasmid - Human IgG3

pFUSE-hIgG3-Fc1 is a cloning plasmid for the generation of a Fc-fusion protein expressing the Fc region (CH2 and CH3 domains) of the human IgG3 heavy chain and the hinge region.

The hinge serves as a flexible spacer between the two parts of the Fc-fusion protein, allowing each part of the molecule to function independently.

Human IgG3 displays high ADCC and CDC.

pFUSE-hIgG3-Fc2 additionally features the IL2 signal sequence for the generation of Fc-Fusion proteins derived from proteins that are not naturally secreted.

All products are for research use only, and not for human or veterinary use.

SPECIFICATIONS

Specifications

Applications

In vitro transfection, Fc-fusion protein generation

Plasmid backbone
pFUSE
Antibiotic resistance
Zeocin®
Gene promoter
Human EF1α/HTLV hybrid promoter
Purification
Ion-exchange chromatography
Appearance (form)
Translucent film
Appearance (color)
Colorless
Reconstitution buffer
Sterile water (not provided)
Tested applications

In vitro transfection

Quality control

Plasmid construct is confirmed by restriction analysis and full-length open reading frame (ORF) sequencing.

Additional information

Effector Activities : ADCC+++, CDC ++++

Protein A binding: -

Protein G binding: ++++

CONTENTS

Contents

  • Product: 
    pFUSE-hIgG3-Fc1
  • Cat code: 
    pfuse-hg301fc1
  • Quantity: 
    20 µg
Includes:

1 ml of Zeocin® (100 mg/ml)

Shipping & Storage

  • Shipping method:  Room temperature

    • Storage:

    • Upon receipt, store product at -20 °C.
    Stability: Reconstituted product is stable for at least 1 year at -20 °C.

DOCUMENTS

Documents

pFUSE-hIgG3-Fc1

Technical Data Sheet

Plasmid Sequence

Safety Data Sheet

Certificate of analysis

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