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pDRIVE-SV40 / Tyr

Product Unit size Cat. code Docs. Qty. Price

pDRIVE-SV40-mTyr

pDRIVE bearing mouse SV40 / Tyr promoter - LacZ

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20 µg

pdrive-sv40mtyr
+-
$554

pDRIVE5SEAP-SV40-mTyr

pDRIVE bearing mouse SV40 / Tyr promoter - SEAP

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20 µg

pdrive5s-sv40mtyr
+-
$554

pDRIVE5Lucia-SV40-mTyr

pDRIVE bearing mouse SV40 / Tyr promoter - Lucia

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20 µg

pdrive5lc-sv40mtyr
+-
$554
  • About
  • Specifications
  • pDRIVE contents
  • Details

SV40 enhancer and Tyrosinase promoter in pDRIVE expression plasmid

Tyrosinase, the rate-limiting enzyme in melanin synthesis, is expressed specifically in pigment-producing cells. The expression of tyrosinase is regulated by cyclic AMP (cAMP).

The M-box 70-bp upstream from the TATA-box and the E-box located downstream of the TATA-box, near the initiator site, are involved in the regulation of the tyrosinase promoter activity by cAMP [1].

Melanoma-specific expression of a transgene can be achieved by using the tyrosinase promoter [2], this targeted expression being further amplified by the addition of the melanocyte-specific enhancer.

 

References:

1. Bertolotto C. et al. 1996. Regulation of tyrosinase gene expression by cAMP in B16 melanoma cells involves two CATGTG motifs surrounding the TATA box: implication of the microphthalmia gene product. J Cell Biol. 134(3):747-55.
2. Vile R. et al. 1994. A comparison of the properties of different retroviral vectors containing the murine tyrosinase promoter to achieve transcriptionally targeted expression of the HSVtk or IL-2 genes. Gene Ther. 1(5):307-16.

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Specifications

pDRIVE bearing mouse SV40 / Tyr promoter - LacZ, SEAP or Lucia
Gene Promoter: Tyr : Tyrosinase
Specificity: Melanocytes & melanoma
Backbone: pDRIVE, pDRIVE5-SEAP or pDRIVE5-Lucia
Selection: Zeocin™

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pDRIVE contents

  • 20 µg of lyophilized DNA
  • 1 ml of Zeocin™ (100 mg/ml)

room temperature Product is shipped at room temperature

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