Invivogen
Menu

pDRIVE-SV40 / AFP

pDRIVE-SV40-hAFP Unit size Cat. code Docs Qty Price
pDRIVE bearing human SV40 / AFP promoter - LacZ
20 µg
pdrive-sv40hafp
+-
$433.00
pDRIVE5SEAP-SV40-hAFP Unit size Cat. code Docs Qty Price
pDRIVE bearing human SV40 / AFP promoter - SEAP
20 µg
pdrive5s-sv40hafp
+-
$433.00
pDRIVE5Lucia-SV40-hAFP Unit size Cat. code Docs Qty Price
pDRIVE bearing human SV40 / AFP promoter - Lucia
20 µg
pdrive5lc-sv40hafp
+-
$433.00

pDRIVE bearing human SV40 / AFP promoter - LacZ, SEAP or Lucia
Gene Promoter: AFP: Alpha-Fetoprotein
Specificity: Hepatocellular carcinoma
Backbone: pDRIVE, pDRIVE5-SEAP or pDRIVE5-Lucia
Selection: Zeocin™

Back to the top

Contents

- Provided as 20 µg lyophilized DNA.
- 4 pouches of E. coli Fast-Media® Zeo (2 TB and 2 Agar).

Back to the top

Description

The alpha-fetoprotein (AFP) gene is normally expressed in fetal but not adult livers. However, about 70% of hepatocellular carcinoma (HCC) are known to overexpress AFP, this up-regulation occuring at the transcriptional level. The AFP promoter has been extensively studied and shown to confer selective expression of a transgene in vitro and in vivo [1]. The expression level of the transgene is proportional to the level of AFP expression in the transfected cells [2]. Several studies have reported the use of AFP promoter to express a cytokine or suicide gene into HCC cells after delivery with viral or plasmid vectors [1, 2]. 

1. Kanai F. et al. 1997. In vivo gene therapy for alpha-fetoprotein-producing hepatocellular carcinoma by adenovirus-mediated transfer of cytosine deaminase gene. Cancer Res. 57(3):461-5.
2. He P. et al. 2000. The targeted expression of interleukin-2 in human hepatocellular carcinoma cells. J Exp Clin Cancer Res. 19(2):183-7.

Back to the top

Details

pDRIVE map

pDRIVE5-SEAP map

Back to the top

Customer Service
& Technical Support

CAPTCHA
In order to prevent automated spam submissions, please answer the following question:
6 + 4 =
Solve this simple math problem and enter the result. E.g. for 1+3, enter 4.
Shopping cart is empty