pDRIVE-GPIIb

GPIIb promoter (Megakaryocytes) in pDRIVE expression plasmid

Integrin αIIb is the alpha-subunit of the alpha IIb/beta 3 complex found on the surface of platelets. The GPIIb gene encoding integrin alpha IIb is only expressed on maturing megakaryocyes and the platelets derived from them.

An 800 bp fragment located upstream of the initiation start site of the αIIb gene contains cis-acting elements necessary for lineage specific expression [1].

This promoter fragment, which lacks a TATA box, binds GATA and Ets factors to induce high level, megakaryocyte-targeted gene transcription in human cells, rat cells and transgenic mice [2].

References:

1. Uzan G. et al., 1991. Tissue-specific expression of the platelet GPIIb gene. J Biol Chem. 266(14):8932-9.
2. Wilcox DA. et al., 1999. Integrin alphaIIb promoter-targeted expression of gene products in megakaryocytes derived from retrovirus-transduced human hematopoietic cells. Proc Natl Acad Sci U S A. 96(17):9654-9.