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pDRIVE-AFP / hAFP

Product Unit size Cat. code Docs. Qty. Price

pDRIVE-AFP-hAFP

pDRIVE bearing human AFP / hAFP promoter - LacZ

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20 µg

pdrive-afphafp
+-
$554

pDRIVE5SEAP-AFP-hAFP

pDRIVE bearing human AFP / hAFP promoter - SEAP

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20 µg

pdrive5s-afphafp
+-
$554

pDRIVE5Lucia-AFP-hAFP

pDRIVE bearing human AFP / hAFP promoter - Lucia

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20 µg

pdrive5lc-afphafp
+-
$554

Alpha-fetoprotein (AFP) enhancer and promoter in pDRIVE expression plasmid

The alpha-fetoprotein (AFP) gene is normally expressed in fetal but not adult livers. However, about 70% of hepatocellular carcinoma (HCC) are known to overexpress AFP, this up-regulation occurring at the transcriptional level.

The AFP promoter has been extensively studied and shown to confer a selective expression of a transgene in vitro and in vivo [1]. The expression level of the transgene is proportional to the level of AFP expression in the transfected cells [2].

Several studies have reported the use of AFP promoter to express a cytokine or suicide gene into HCC cells after delivery with viral or plasmid vectors [1, 2]. 

 

References:

1. Kanai F. et al. 1997. In vivo gene therapy for alpha-fetoprotein-producing hepatocellular carcinoma by adenovirus-mediated transfer of cytosine deaminase gene. Cancer Res. 57(3):461-5.
2. He P. et al. 2000. The targeted expression of interleukin-2 in human hepatocellular carcinoma cells. J Exp Clin Cancer Res. 19(2):183-7.

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Specifications

pDRIVE bearing human AFP / hAFP promoter - LacZ, SEAP or Lucia
Gene Promoter: AFP: Alpha-Fetoprotein
Specificity: Hepatocellular carcinoma
Backbone: pDRIVE, pDRIVE5-SEAP or pDRIVE5-Lucia
Selection: Zeocin™

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pDRIVE contents

  • 20 µg of lyophilized DNA
  • 1 ml of Zeocin™ (100 mg/ml)

room temperature Product is shipped at room temperature

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Details

pDRIVE map

pDRIVE5-SEAP map

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Citations

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