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ODN 2007 - TLR9 ligand

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ODN 2007

TLR9 Agonist - Stimulatory CpG ODN, Class B

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1 mg

5 mg

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tlrl-2007-1
+-
$624

Class B CpG oligonucleotide - Bovine / porcine TLR9 ligand

TLR9 activation with ODN 2007
TLR9 activation with ODN 2007

ODN 2007 is a Class B CpG oligonucleotide (ODN). It is a short synthetic single-stranded DNA molecule containing unmethylated CpG dinucleotides (CpG motifs). These unmethylated CpG motifs mimic microbial DNA and act as immunostimulants. ODN 2007 is a ligand of choice for bovine and porcine Toll-like receptor 9 (TLR9). 

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Mode of action:

Stimulatory CpG ODNs are internalized and activate the endosomal receptor TLR9. Activation of TLR9 triggers NF-κB- and interferon regulatory factor (IRF)-mediated pro-inflammatory responses upon the recognition of unmethylated cytosine-phosphorothioate-guanosine (CpG) forms of DNA [1-3]. Unmethylated CpG dinucleotides are a hallmark of microbial (bacterial, viral, fungal, and parasite) DNA, as well as mitochondrial self-DNA [3, 4]. Class B (also called Type K) CpG ODNs, such as ODN 2007, contain a full phosphorothioate backbone with one or more CpG dinucleotides. They strongly activate B cells but weakly stimulate IFN-α secretion in plasmacytoid dendritic cells [5].

 

Key features of ODN 2007:

  • Potent activator of TLR9
  • Synthetic ODN with unmethylated CpG motifs
  • Each lot is functionally tested

 

Get more information about CpG ODNs Classes.

 

Read our review Read our review on TLR9 agonists: double-edged sword for immune therapies.

 

 

References

1. Kumagai Y. et al., 2008. TLR9 as a key receptor of the recognition of DNA. Adv. Drug. Deliv. Rev. 60(7):795-804.
2. Heinz L.X. et al., 2021. TASL is the SLC15A4-associated adaptor for IRF5 activation by TLR7-9. Nature. 581(7808):316-322.
3. Kayraklioglu N. et al., 2021. CpG oligonucleotides as vaccine adjuvants. DNA Vaccines: Methods and Protocols. Methods in Molecular Biology. Vol. 2197. p51-77.
4. Kumar V., 2021. The trinity of cGAS, TLR9, and ALRs: guardians of the cellular galaxy against host-derived self-DNA. Front. Immunol. 11:624597.
5. Krieg A.M. et al., 1995. CpG motifs in bacterial DNA trigger direct B-cell activation. Nature. 374(6522):546-9.

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Specifications

Specificity: bovine / porcine TLR9 agonist

Working concentration: 5 µM (10 μg/ml)

Solubility:  5 mg/ml in water

ODN 2007 sequence
5’- tcg tcg ttg tcg ttt tgt cgt t -3’ (22 mer)
Note: Bases are phosphorothioate (nuclease resistant).

Quality control:

  • TLR9 activity has been tested using cellular assays.
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Contents

ODN 2007 is provided lyophilized and is available in two quantities.

tlrl-2007-1 (formerly tlrl-podnb-1):

  • 1 mg (141.65 nmol) lyophilized ODN 2007
  • 1.5 ml sterile endotoxin-free water

tlrl-2007-5 (formerly tlrl-podnb-5):

  • 5 mg (708.25 nmol) lyophilized ODN 2007
  • 10 ml sterile endotoxin-free water

room temperature ODN 2007 is shipped at room temperature.

store Upon receipt, store at -20 °C.

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Details

Toll-Like Receptor 9 (TLR9) is an endosomal receptor that triggers NF-κB- and interferon regulatory factor (IRF)-mediated pro-inflammatory responses upon the recognition of unmethylated cytosine-phosphorothioate-guanosine (CpG) forms of DNA [1-3]. Unmethylated CpG dinucleotides are a hallmark of microbial (bacterial, viral, fungal, and parasite) DNA, as well as mitochondrial self-DNA [3,4]. These TLR9 agonists can be mimicked by synthetic oligonucleotides containing CpG motifs (CpG ODNs), which have been extensively studied to improve adaptive immune responses in the context of vaccination [1,3].

TLR9 is mainly expressed in subsets of Dendritic Cells and in B cells of all mammals. In rodents, but not in humans, TLR9 is also expressed in monocytes and macrophages [3]. The structure of the receptor varies by 24% between human TLR9 (hTLR9) and mouse TLR9 (mTLR9) [3]. They recognize different CpG motifs, the optimal sequences being GTCGTT and GACGTT for hTLR9 and mTLR9, respectively [5].
 

More Information Get more information about CpG-ODNs Classes.

 

References

1. Kumagai Y. et al., 2008. TLR9 as a key receptor of the recognition of DNA. Adv. Drug. Deliv. Rev. 60(7):795-804.
2. Heinz L.X. et al., 2021. TASL is the SLC15A4-associated adaptor for IRF5 activation by TLR7-9. Nature. 581(7808):316-322.
3. Kayraklioglu N. et al., 2021. CpG oligonucleotides as vaccine adjuvants. DNA Vaccines: Methods and Protocols. Methods in Molecular Biology. Vol. 2197. p51-77.
4. Kumar V., 2021. The trinity of cGAS, TLR9, and ALRs: guardians of the cellular galaxy against host-derived self-DNA. Front. Immunol. 11:624597.
5Bauer S. et al., 2001. Human TLR9 confers responsiveness to bacterial DNA via species-specific CpG motif recognition. Proc Natl Acad Sci USA, 98(16):9237-42.

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