InvivoGen provides an HEK293-derived cell line to study the oxidative stress.
Oxidative stress is a phenomenon caused by an imbalance between the production and accumulation of reactive oxygen species (ROS) and the ability of the body to detoxify these reactive products. While ROS play essential roles in cell signaling and homeostasis, excessive amounts can damage cellular components and be responsible in the onset and/or progression of nearly all major human diseases, including diabetes, atherosclerosis, aging, and cancer. Antioxidant defense systems have evolved as a means of protection against oxidative stress, with the substrate adaptor protein KEAP1 as the oxidative stress sensor and the transcription factor Nrf2 as the key regulator. Activation of KEAP1 leads to the nuclear translocation of Nrf2, which in turn binds to the antioxidant response element (ARE) found within the promoter region of many antioxidant genes leading to their transcription.
InvivoGen provides an HEK293-derived oxidative stress reporter cell line featuring an ARE-inducible Lucia reporter gene. Expression of Lucia is proportional to the oxidative stress response. It can be readily assessed using the reporter detection reagent QUANTI-Luc 4 Lucia/Gaussia.