Anti-CD20 (Rituximab biosimilar - mIgG1 isotype)
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Human CD20 (Rituximab) antibody - Murine IgG1
Murine IgG1 monoclonal antibody (mAb) against human CD20
Anti-hCD20-mIgG1 features the constant region of the mouse IgG1 isotype and the variable region of rituximab.
Rituximab is a mouse/human chimeric monoclonal antibody that targets the CD20 antigen found on the surface of malignant and normal B lymphocytes.
The binding of rituximab to CD20 results in cell destruction through different mechanisms including direct signaling of apoptosis, complement activation, and cell-mediated cytotoxicity.
Rituximab has been approved by the FDA to treat various lymphoid malignancies, including B-cell non-Hodgkin's lymphoma and B-cell chronic lymphocytic leukemia.
Anti-hCD20-mIgG1 was generated by recombinant DNA technology. It has been produced in Chinese hamster ovary (CHO) cells and purified by affinity chromatography with protein G.
More isotypes of this antibody are available and can be used for comparison of biological activities such as ADCC (see below or in the 'upon request' section). InvivoGen also provides type I and type II anti-hCD20 mAbs featuring the variable regions of Ofatumumab or Obinutuzumab, with either native or engineered Fc regions.Back to the top
Target: Human CD20
Clonality: Monoclonal antibody
Clone: Rituximab (Anti-hCD20-hIgG1, kappa)
Isotype: Mouse IgG1, kappa
Control: Mouse IgG1
Source: CHO cells
Formulation: 0.2 µm filtered solution in 68 mM sodium phosphate buffer (pH 7.4) with 91 mM glycine, 5% w/v saccharose, and stabilizing agents
Purity: Purified by affinity chromatography with protein G
Tested application: Flow cytometry and ADCC
- Binding of Anti-hCD20-mIgG1 to human CD20 has been tested using flow cytometry.
- The complete sequence of this antibody has been verified.
- The absence of bacterial contamination (e.g. lipoproteins and endotoxins) has been confirmed using HEK-Blue™ TLR2 and HEK-Blue™ TLR4 cells.
- 100 µg purified anti-hCD20-mIgG1 antibody, provided azide-free and lyophilized.
Product is shipped at room temperature.
Store lyophilized antibody at -20°C.
Lyophilized product is stable for 1 year.Back to the top