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Jurkat-Raji PD-1/PD-L2 Antagonist Assay (Bio-IC™)

Two cell line bioassay - B lymphocytes & NFAT-Lucia reporter T lymphocytes

Validation of human PD-1 expression by Jurkat-Lucia™ TCR-hPD-1 cells.

Validation of human PD-L1 and PD-L2 expression by Raji-APC-hPD-L2 cells

Activation of Jurkat-Lucia™ TCR-hPD-1 cells using Anti-hPD-1 biosimilar mAbs

Activation of Jurkat-Lucia™ TCR-hPD-1 cells using Anti-hPD-L1 or Anti-hPD-L2 mAbs, alone or in combination

Jurkat-Raji (PD-1/CTLA4)/PD-L1 Antagonist Assay (Bio-IC™)

Two cell line bioassay - B lymphocytes & NFAT-Lucia reporter T lymphocytes

Validation of human CD28, CTLA4, and PD-1 expression by Jurkat-Lucia™ TCR-hCTLA4-hPD-1 cells

Validation of human CD80 and PD-L1 expression by Raji-APC-hPDL1-derived cells

Activation of Jurkat-Lucia™ TCR-hCTLA4-hPD-1 cells using Anti-hPD-1 and Anti-hCTLA4 biosimilar mAbs, alone or in combination

Jurkat-Raji CTLA4/CD80 Antagonist Assay (Bio-IC™)

Two cell line bioassay - B lymphocytes & NFAT-Lucia reporter T lymphocytes

Validation of human CD28 and CTLA4 overexpression by Jurkat-Lucia™ TCR-hCTLA4 cells

Validation of endogenous human CD80 expression by Raji-APC-Null cells

ation of Jurkat-Lucia™ TCR-hCTLA4 cells using Anti-hCTLA4 biosimilar mAb

NF-κB and ISG Dual Reporter A549 Cells

NF-κB-SEAP & IRF-Lucia reporter lung carcinoma

NF-κB induction in A549-Dual™ cells

IRF induction in A549-Dual™ cells

Human MDA5-KO Dual Reporter A549 Cells

NF-κB-SEAP & IRF-Lucia reporter lung carcinoma

Validation of MDA-5 knockout by Western blot (Wes™)

IRF INDUCTION (Lucia luciferase reporter)

NF-κB INDUCTION (SEAP reporter)

Human RIG-I-KO Dual Reporter A549 Cells

NF-κB-SEAP & IRF-Lucia reporter lung carcinoma

Validation of RIG-I knockout by Western blot (Wes™)

IRF INDUCTION

NF-kB INDUCTION

IFN-γ Reporter B16 Cells

STAT1-SEAP reporter mouse melanoma

Dose-response of B16-Blue™ IFN-γ cells to recombinant murine IFN-γ.

Response of B16-Blue™ IFN-γ cells to a panel of cytokines

Mouse STING-KO ISG Reporter B16 Cells

IRF-SEAP reporter mouse melanoma -STING deficient

Validation of IRF5 knockout by Western blot (Wes™)

Response of B16-Blue ISG and B16-Blue ISG-KO-STING to CDNs and IFN-β

ISG Reporter B16 Cells

IRF-SEAP reporter mouse melanoma

RIG-I response to 5`ppp-dsRNA and 5`ppp-dsRNA Control in B16-Blue™ ISG cells

Response of B16-Blue ISG and B16-Blue ISG-KO-STING to CDNs and IFN-β

IFN-α/β Reporter B16 Cells

STAT1/2-SEAP reporter mouse melanoma

Dose-response of B16-Blue™ IFN‑α/β cells to recombinant murine IFN‑α/β

Response of B16-Blue™ IFN-α/β cells to a panel of cytokines

Jurkat & Raji PD-1/PD-L1 Antagonist Assay (Bio-IC™)

Two cell line bioassay - B lymphocytes & NFAT-Lucia reporter T lymphocytes

CD28 and PD-1 expression on Jurkat-Lucia™ TCR-hPD-1 cells

PD-L1 expression on Raji-APC-derived cells

Disruption of PD-1/PD-L1 inhibitory interaction using Anti-hPD-1 or Anti-hPD-L1 antibodies

Human ACE2 & TMPRSS2 & NLRP1 & ASC-GFP Reporter A549 Cell Lines

Two cell lines (ACE2-OE & TMPRSS2-OE w/o NLRP1-OE) - ASC-GFP speck forming lung carcinoma

Overexpression of TMPRSS2 (qRT-PCR)

Cell surface expression of ACE2 (flow-cytometry)

NLRP1 and ASC-GFP expression (Western blot)

ASC speck formation upon NLRP1 inflammasome activation (with hTNF-α stimulation)

ASC speck formation upon NLRP1 inflammasome activation (without hTNF-α stimulation)

Pyroptotic cell death upon NLRP1 inflammasome activation

Jurkat & Raji ICOS/ICOS-L Antagonist Assay (Bio-IC™)

Two cell line bioassay - B lymphocytes & NFAT-Lucia reporter T lymphocytes

ICOS overexpression on Jurkat-Lucia™ hICOS cells

ICOS-L overexpression on Raji-hICOS-L cells

Disruption of ICOS/ICOS-L activatory activation

Jurkat & Raji CD27/CD70 Antagonist Assay (Bio-IC™)

Two cell line bioassay - B lymphocytes & NF-κB-Lucia reporter T lymphocytes

CD27 expression on Jurkat-Lucia™ hCD27 cells

CD70 expression on Raji-Null cells

Disruption of CD27/CD70 activatory interaction

Jurkat & Raji 4-1BB/4-1BBL Antagonist Assay (Bio-IC™)

Two cell line bioassay - B lymphocytes & NF-κB-Lucia reporter T Lymphocytes

4-1BB expression on Jurkat-Lucia™ h4-1BB cells

4-1BBL expression on Raji-Null cells

Disruption of 4-1BB/4-1BBL activatory interaction

Human ACE2 & TMPRSS2 A549 Cell Lines

Two cell lines (ACE2-OE w/o TMPRSS2-OE) - Lung carcinoma

Validation of ACE2 overexpression by RT-qPCR

Validation of ACE2 surface expression on A549-hACE2 cells by FACS

Infection of A549-hACE2 cells by SARS-CoV-2 Spike pseudotyped lentiviral particles

Validation of ACE2 and TMPRSS2 overexpression by RT-qPCR

Validation of ACE2 surface expression on A549-hACE2-TMPRSS2 cells by FACS

Infection of A549-hACE2-TMPRSS2 cells by SARS-CoV-2 Spike pseudotyped lentiviral particles

Human NLRP1 & ASC-GFP Reporter A549 Cell Lines

Two cell lines (w/o NLRP1-OE) - ASC-GFP speck forming lung carcinoma

Validation of NLRP1 and inducible ASC::GFP expression

Validation of inducible ASC::GFP expression

Monitoring of ASC speck formation and pyroptosis upon NLRP1 activation using Val-boroPro

Monitoring of ASC speck formation and pyroptosis upon NLRP1 activation using Poly(IC)

Human MAVS-KO Dual Reporter A549 Cells

NF-κB-SEAP & IRF-Lucia reporter lung carcinoma

IRF INDUCTION (Lucia luciferase reporter)

NF-κB INDUCTION (SEAP reporter)