Anti-mMincle Detection and Neutralizing mAb

Murine Mincle Detection and Neutralizing antibody - Monoclonal Rat IgG2b

Neutralizing and detection antibody against mMincle

Anti-mMincle-IgG (clone 6G5) is a monoclonal rat IgG2b antibody specific for murine Mincle (mMincle). This antibody was screened for neutralization activity and flow cytometry. It is produced in hybridomas and purified by affinity chromatography with protein G.

Mincle is a C-type lectin receptor that has emerged as an important player in innate immunity. This receptor recognizes a variety of exogenous and endogenous stimuli, such as mycobacteria, some fungi, and necrotic cells [1, 2].

More details

Key features:

• Targets murine Mincle
• Provided azide-free
• Each lot is functionally tested

Read our review on C-Type Lectin Receptors

References:

1. Yamasaki S. et al., 2009. C-type lectin Mincle is an activating receptor for pathogenic fungus, Malassezia. PNAS 106(6): 1897–1902
2. Brown G.D., 2008. Sensing necrosis with Mincle. Nature Immunol. 9:1099-1100.

Specifications

Target: Murine Mincle (mMincle)

Specificity: No cross-reactivity with human Mincle

Clonality: Monoclonal antibody

Clone: 6G5

Isotype: Rat IgG2b

Control: Rat IgG2b Control

Source: Hybridoma cells

Formulation: 0.2 µm filtered solution in a sodium phosphate buffer with saccharose, glycine, and stabilizing agents.

Purity: Purified by affinity chromatography with protein G

Applications: Neutralization/block; Flow cytometry

Quality control:

• This product has been validated for neutralization using cellular assays.
• Binding of Anti-mMincle-IgG to mMincle on cells has been validated using flow cytometry.
• The absence of bacterial contamination (e.g. lipoproteins and endotoxins) has been confirmed using HEK-Blue™ TLR2 and HEK‑Blue™ TLR4 cells.

Contents

• 100 μg of purified Anti-mMincle-IgG, provided azide-free and lyophilized

Product is shipped at room temperature.

Upon receipt, store lyophilized anti-mMincle-IgG at -20°C.

Details

Mincle is a member of the C-type lectin receptor (CLR) family. Mincle recognizes a variety of exogenous and endogenous stimuli, such as mycobacteria, certain fungi, and necrotic cells [1, 2]. Exogenous ligands for Mincle include fungal α-mannose, and the mycobacterial glycolipid, trehalose-6’6’-dimycolate (TDM), also known as cord factor the immunostimulatory component of Mycobacterium tuberculosis [3].
Mincle also binds trehalose-6,6-dibehenate (TDB) which is a synthetic analog of TDM. Furthermore, Mincle senses damaged cells by recognizing endogenous damage-associated molecular patterns (DAMPs) [4]. Upon ligand recognition, Mincle interacts with the Fc receptor common γ-chain (FcRγ), which triggers intracellular signaling through Syk leading to CARD9-dependent NF-κB activation. Syk also induces the mobilization of intracellular calcium (Ca²⁺) and the activation of the calcineurin-NFAT pathway.

References:

1. Yamasaki S. et al., 2009​. C-type lectin Mincle is an activating receptor for pathogenic fungus, Malassezia. PNAS 106(6): 1897–1902. 
2. Brown GD. 2008.  Sensing necrosis with Mincle. Nature Immunol. 9:1099-1100. 
3. Ishikawa E. et al., 2009. Direct recognition of the mycobacterial glycolipid, trehalose dimycolate, by C-type lectin Mincle. J Exp Med. 206(13):2879-88.
4. Yamasaki S. et al., 2008. Mincle is an ITAM-coupled activating receptor that senses damaged cells. Nat Immunol. 9(10):1179-88.