Mouse gp75 Antibody (clone TA99)

Recombinant mouse gp75 antibody for in vivo use

Anti-mgp75-mIgG2a InvivoFit™ is a recombinant mouse IgG2a monoclonal antibody (mAb) based on the previously described clone TA99. This clone reacts against the human and mouse tumor-associated antigen antigen glycoprotein 75 (gp75) [1]. The sequence of Anti-mgp75-mIgG2a InvivoFit™ is 100% murine (constant and variable regions), thus ensuring minimal immunogenicity upon repeated injections into mice. This mAb efficiently induces protective immunity against B16F10 melanoma, either subcutaneous or metastatic [2]. Of note, the mIgG2a isotype was shown to be the most potent at inducing antibody-dependent cellular cytotoxicity compared to mIgG1, mIgG2b, and mIgG3 [3]. As opposed to the original hybridoma-based production which contains a mixed population of anti-gp75 antibodies featuring correct or aberrant light chains [4], InvivoGen produces the recombinant anti-mgp75-mIgG2a mAb using Chinese hamster ovary (CHO) cells.
Anti-mgp75-mIgG2a is purely monoclonal and is provided in an InvivoFit™ grade, a high-quality standard specifically adapted to in vivo studies.

Key features of Anti-mgp75-mIgG2a InvivoFit™:

• Sequence is 100% murine
• Free from non-relevant mAbs found in hybridoma-based productions
• Filter-sterilized (0.2 µm), endotoxin level < 1 EU/mg
• Suitable for parenteral delivery in mice (azide-free)
• Low aggregation < 5%
• Produced in animal-free facilities and defined media

Anti-mgp75-mIgG2a InvivoFit™ is purified by affinity chromatography with protein A. Its binding to mouse gp-75 is validated using ELISA.

References:

1. Thomson T.M. et al., 1985. Pigmentation-associated glycoprotein of human melanomas and melanocytes: definition with a mouse monoclonal antibody. J Invest Dermatol. 85(2):169-74.
2. Hara I. et al., 1995. Implicating a role for immune recognition of self in tumor rejection: passive immunization against the brown locus protein. J Exp Med. 182(5):1609-14.
3. Nimmerjahn F. & Ravetch J.V., 2005. Divergent immunoglobulin g subclass activity through selective Fc receptor binding. Science. 310(5753):1510-2.
4. Boross P. et al., 2014. Anti-tumor activity of human IgG1 anti-gp75 TA99 mAb against B16F10 melanoma in human FcgammaRI transgenic mice. Immunol Lett. 160(2):151-7.