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Anti-hCD20-hIgG1

Anti-hCD20-hIgG1 Unit size Cat. code Docs Qty Price
Human CD20 (rituximab) antibody - Human IgG1
100 µg
hcd20-mab1
+-
$348.00

You may also need : Anti-β-Gal-hIgG1 | View more associated products

Monoclonal human IgG1 antibody against human CD20

Effector functions of mAb isotypes targeting CD20

Anti-hCD20-hIgG1 features the constant region of the human IgG1 isotype and the variable region of rituximab.

Rituximab is a mouse/human chimeric monoclonal antibody that targets the CD20 antigen found on the surface of malignant and normal B lymphocytes.

Binding of rituximab to CD20 results in cell destruction through different mechanisms including direct signaling of apoptosis, complement activation and cell-mediated cytotoxicity.

Rituximab has been approved by the FDA for the treatment of various lymphoid malignancies, including B-cell non-Hodgkin's lymphoma and B-cell chronic lymphocytic leukaemia.

Anti-hCD20-hIgG1 was generated by recombinant DNA technology. It has been produced in CHO cells and purified by affinity chromatography with protein G.

 

Figures

ADCC assay using various anti-human CD20 (Rituximab) antibody isotypes and Raji-Null target cells
ADCC assay using various anti-human CD20 (Rituximab) antibody isotypes and Raji-Null target cells

Comparison of ADCC potency for native and engineered anti-human CD20 antibody isotypes: Raji-Null cells were incubated with gradient concentrations of Anti-hCD20 or Anti-β-galactosidase (β-gal) mAbs for 1 hour. Jurkat-Lucia™ NFAT-CD16 effector cells were then co-incubated with targets cells for 6 hours. NFAT activation, reflecting the induced ADCC response, was assessed by determining Lucia luciferase activity in the supernatant using QUANTI-Luc™. Percentages of the maximal response normalized to the IgG1 isotype are shown

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Specifications

Clonality: Monoclonal antibody

Specificity: Targets cells expressing human CD20

Isotype: Human IgG1, kappa

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Contents

100 µg purified anti-hCD20-hIgG1 antibody, provided azide-free and lyophilized.

Product is shipped at room temperature.

Store lyophilized antibody at -20°C.

Lyophilized product is stable for 2 years

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Citations

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