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Jurkat-Raji PD-1/PD-L2 Antagonist Assay (Bio-IC™)

Two cell line bioassay - B lymphocytes & NFAT-Lucia reporter T lymphocytes

Validation of human PD-1 expression by Jurkat-Lucia™ TCR-hPD-1 cells.

Validation of human PD-L1 and PD-L2 expression by Raji-APC-hPD-L2 cells

Activation of Jurkat-Lucia™ TCR-hPD-1 cells using Anti-hPD-1 biosimilar mAbs

Activation of Jurkat-Lucia™ TCR-hPD-1 cells using Anti-hPD-L1 or Anti-hPD-L2 mAbs, alone or in combination

Jurkat-Raji (PD-1/CTLA4)/PD-L1 Antagonist Assay (Bio-IC™)

Two cell line bioassay - B lymphocytes & NFAT-Lucia reporter T lymphocytes

Validation of human CD28, CTLA4, and PD-1 expression by Jurkat-Lucia™ TCR-hCTLA4-hPD-1 cells

Validation of human CD80 and PD-L1 expression by Raji-APC-hPDL1-derived cells

Activation of Jurkat-Lucia™ TCR-hCTLA4-hPD-1 cells using Anti-hPD-1 and Anti-hCTLA4 biosimilar mAbs, alone or in combination

Jurkat-Raji CTLA4/CD80 Antagonist Assay (Bio-IC™)

Two cell line bioassay - B lymphocytes & NFAT-Lucia reporter T lymphocytes

Validation of human CD28 and CTLA4 overexpression by Jurkat-Lucia™ TCR-hCTLA4 cells

Validation of endogenous human CD80 expression by Raji-APC-Null cells

ation of Jurkat-Lucia™ TCR-hCTLA4 cells using Anti-hCTLA4 biosimilar mAb

NF-κB Reporter Ramos Cells (MyD88 deficient)

NF-κB-SEAP reporter B lymphocytes

MyD88 dependent and independent responses in Ramos-Blue™ and Ramos-Blue™-defMyD

NF-κB Reporter Ramos Cells

NF-κB-SEAP reporter B lymphocytes

NF-kB/AP-1 activation in Ramos-Blue™ cells induced by various activators

MyD88 dependent and independent responses in Ramos-Blue™ and Ramos-Blue™-defMyD

Control APC Raji Cells

Human lymphoblast cells - Bio-IC™ control cells

Activation of Jurkat-Lucia TCR-hPD-1 cells using Raji-APC-Null or Raji-APC-hPD-L1 cells

Jurkat & Raji PD-1/PD-L1 Antagonist Assay (Bio-IC™)

Two cell line bioassay - B lymphocytes & NFAT-Lucia reporter T lymphocytes

CD28 and PD-1 expression on Jurkat-Lucia™ TCR-hPD-1 cells

PD-L1 expression on Raji-APC-derived cells

Disruption of PD-1/PD-L1 inhibitory interaction using Anti-hPD-1 or Anti-hPD-L1 antibodies

Jurkat & Raji ICOS/ICOS-L Antagonist Assay (Bio-IC™)

Two cell line bioassay - B lymphocytes & NFAT-Lucia reporter T lymphocytes

ICOS overexpression on Jurkat-Lucia™ hICOS cells

ICOS-L overexpression on Raji-hICOS-L cells

Disruption of ICOS/ICOS-L activatory activation

Jurkat & Raji CD27/CD70 Antagonist Assay (Bio-IC™)

Two cell line bioassay - B lymphocytes & NF-κB-Lucia reporter T lymphocytes

CD27 expression on Jurkat-Lucia™ hCD27 cells

CD70 expression on Raji-Null cells

Disruption of CD27/CD70 activatory interaction

Jurkat & Raji 4-1BB/4-1BBL Antagonist Assay (Bio-IC™)

Two cell line bioassay - B lymphocytes & NF-κB-Lucia reporter T Lymphocytes

4-1BB expression on Jurkat-Lucia™ h4-1BB cells

4-1BBL expression on Raji-Null cells

Disruption of 4-1BB/4-1BBL activatory interaction

Human EGFR-expressing Raji Cells

Human lymphoblast cells - ADCC EGFR Target Cells

Validation of hEGFR expression by flow cytometry

Comparison of ADCC potency for native and engineered anti-human EGFR antibody isotypes

Human TRIF-KO Dual Reporter THP-1 Cells

NF-κB-SEAP and IRF-Lucia reporter monocytes

Validation of TRIF KO

IRF responses in PMA-differentiated THP1-Dual™ KO-TRIF cells

NF-κB responses in PMA-differentiated THP1-Dual™ KO-TRIF cells

Human 4-1BB-expressing Raji Cells

Human lymphoblast cells - ADCC 4-1BB Target Cells

Validation of the expression of human 4-1BB by Raji-h4-1BB cells by flow cytometry

Comparison of ADCC potency for native and engineered anti-human 4-1BB antibody isotypes.

Human LAG3-expressing Raji Cells

Human lymphoblast cells - ADCC LAG3 Target Cells

Validation of the expression of human (h)LAG3 by Raji-hLAG3 cells.

Human OX40-expressing Raji Cells

Human lymphoblast cells - ADCC OX40 Target Cells

Validation of the expression of human OX40 by Raji-hOX40 cells.

Comparison of ADCC potency for native and engineered anti-human OX40 antibody isotypes.

Human ICOS-expressing Raji Cells

Human lymphoblast cells - ADCC ICOS Target Cells

Validation of the expression of human ICOS by Raji-hICOS cells by flow cytometry

ADCC assay using various anti-human ICOS antibody isotypes and Raji-hICOS target cells

Human RIG-I-KO Dual Reporter THP-1 Cells

NF-κB-SEAP & IRF-Lucia reporter monocytes

Validation of RIG-I knockout

Functional validation of RIG-I knockout (IRF response)

Functional validation of RIG-I knockout (NF-κB response)

Human VISTA-expressing Raji Cells

Human lymphoblast cells - ADCC VISTA Target Cells

VISTA is expressed by Raji-hVISTA cells

ADCC assay with Raji-hVISTA cells

Control Raji Cells

Human lymphoblast cells - ADCC CD19/CD20/Control Target Cells

ADCC assay using various anti-human CD20 (Rituximab) antibody isotypes and Raji-Null target cells

ADCC induction on antigen-expressing Raji cells and Raji-Null control target cells

Human PD-L1-expressing Raji Cells

Human lymphoblast cells - ADCC PD-L1 Target Cells

Validation of the expression of human PD-L1 by Raji-hPD-L1 cells

Comparison of ADCC potency for native and engineered anti-human PD-L1 antibody isotypes