Ramos-Blue™ Cells
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Cat.code:
rms-sp
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ABOUT
NF-κB/AP-1 reporter B lymphocytes
Ramos-Blue™ cells are B lymphocyte cell lines that stably expresses an NF-κB/AP-1-inducible SEAP (secreted embryonic alkaline phosphatase) reporter gene.
Ramos-Blue™ cells are responsive to NF-κB inducers, such as TNF-α and some TLR agonists (poly(I:C), R848, ODN 2006). When stimulated, they produce SEAP in the supernatant.
The levels of SEAP can be readily monitored using QUANTI-Blue™, a medium that turns purple/blue in the presence of SEAP.
Disclaimer: These cells are for internal research use only and are covered by a Limited Use License (See Terms and Conditions). Additional rights may be available.
SPECIFICATIONS
Specifications
Complete IMDM (see TDS)
Verified using Plasmotest™
Each lot is functionally tested and validated.
CONTENTS
Contents
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Product:Ramos-Blue™ Cells
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Cat code:rms-sp
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Quantity:3-7 x 10^6 cells
- 1 ml of Zeocin® (100 mg/ml)
- 1 ml of Normocin™ (50 mg/ml)
- 1 ml of QB reagent and 1 ml of QB buffer (sufficient to prepare 100 ml of QUANTI-Blue™ Solution)
Shipping & Storage
- Shipping method: Dry ice
- Liquid nitrogen vapor
- Upon receipt, store immediately in liquid nitrogen vapor. Do not store cell vials at -80°C.
Storage:
Caution:
DOCUMENTS
Documents
Technical Data Sheet
Safety Data Sheet
Certificate of analysis
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