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TLR04

pUNO1-bTLR04 Unit size Cat. code Docs Qty Price
pUNO1 bearing the bovine TLR4 gene
20 µg
puno1-btlr4
+-
$384.00
pUNO1-hTLR04a Unit size Cat. code Docs Qty Price
pUNO1 bearing the human TLR4 gene, isoform_1
20 µg
puno1-htlr4a
+-
$384.00
pUNO3-hTLR04a Unit size Cat. code Docs Qty Price
pUNO3 bearing the human TLR4 gene, isoform_1
20 µg
puno3-htlr4a
+-
$384.00
pUNO1-mTLR04 Unit size Cat. code Docs Qty Price
pUNO1 bearing the mouse TLR4 gene
20 µg
puno1-mtlr4
+-
$384.00
pUNO1-pTLR04 Unit size Cat. code Docs Qty Price
pUNO1 bearing the pig TLR4 gene
20 µg
puno1-ptlr4
+-
$384.00

Expression-ready ORF Clones

Alias : TLR4, CD284

Toll Like Receptor 4

TLR4, the first human TLR identified, is the receptor for Gram-negative lipopolysaccharide (LPS). The TLR4 gene was shown to be mutated in C3H/HeJ and C57BL/10ScCr mice, both of which are low responders to lipopolysaccharide (LPS) [1]. However, TLR4 alone is not sufficient to confer LPS responsiveness. TLR4 requires MD-2, a secreted molecule, to functionally interact with LPS [2]. Furthermore, a third protein, called CD14, was shown to participate in LPS signaling, leading to NF-κB translocation. This signaling is mediated through the adapter protein MyD88 but also through a MyD88-independent pathway that involves the (TIR) domain-containing adapter protein (TIRAP) [3].

 

1. Poltorak et al. (1998). Science, 282(5396):2085-8.
2. Shimazu et al. (1999). J Exp Med, 189(11):1777-82
3. Horng et al. (2001). Nat Immunol, 2(9):835-41

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Specifications

Bovine TLR4 (pUNO1-bTLR04)

Genbank : NM_174198.6
ORF size : 2526 bp
Subclone : AgeI - NheI

Human TLR4 isoform 1 (pUNO1-hTLR04a)

ORF size : 2520 bp
Subclone : BspHI - NheI

Human TLR4 isoform 1 (pUNO3-hTLR04a)

ORF size : 2520 bp
Subclone : BspHI - NheI

Mouse TLR4 (pUNO1-mTLR04)

Genbank : JX878358.1
ORF size : 2508 bp
Subclone : BspHI - NheI

Pig TLR4 (pUNO1-pTLR04)

ORF size : 2526 bp
Subclone : AgeI - NheI

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Contents pUNO-TLR

room temperature Product is shipped at room temperature.

store Lyophilized DNA should be stored at -20 ̊C.

stability Resuspended DNA should be stored at -20 ̊C and is stable up to 1 year.

 

pUNO1 plamids:

  • 20 µg of lyophilized DNA
  • 2 x 1 ml blasticidin at 10 mg/ml

Alert Blasticidin is a harmful compound. Refer to safety data sheet for handling instructions.
Store blasticidin at 4°C or -20°C for up to two years. Product is stable 2 weeks at 37°C.
Avoid repeated freeze-thaw cycles.

 

pUNO3 plasmid:

Hygromycin B is a hazardous compound. Avoid contact with skin and eyes.
Store Hygromycin B Gold at 4°C or -20˚C.
Avoid repeated freeze-thaw cycles.

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Citations

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