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TLR03-DN

pUNO1-hTLR03-DN Unit size Cat. code Docs Qty Price
pUNO1 bearing the human TLR3-DN gene
20 µg
puno1-htlr3-dn
+-
$406.00
pUNO1-mTLR03-DN Unit size Cat. code Docs Qty Price
pUNO1 bearing the mouse TLR3-DN gene
20 µg
puno1-mtlr3-dn
+-
$406.00
pUNO1-hTLR03-DN-HA Unit size Cat. code Docs Qty Price
pUNO1 bearing the human TLR3-DN gene, with HA-tag
20 µg
puno1ha-htlr3-dn
+-
$406.00
pUNO1-mTLR03-DN-HA Unit size Cat. code Docs Qty Price
pUNO1 bearing the mouse TLR3-DN gene, with HA-tag
20 µg
puno1ha-mtlr3-dn
+-
$406.00

Expression-ready ORF Clones

Alias : TLR3, CD283

TLR3-DN (ΔTIR) : Dominant Negative TIR-less TLR3 gene

 

TLR3 recognizes double-stranded RNA (dsRNA), a molecular pattern associated with viral infection. Stimulation with polyinosine-polycytidylic acid (poly(I:C)), a synthetic analog of dsRNA, was shown to induce hyporesponsiveness in TLR3-deficient mice and marked responsiveness only in 293 cells expressing TLR3 [1], suggesting a specific recognition to poly(I:C) by TLR3.
TLR3 signaling is not elicited by either single-stranded RNA (ssRNA) or dsDNA [2]. TLR3 activation induces cytokine production through a signaling pathway dependent on MyD88. Moreover, poly(I:C) can induce activation of NF-kB and mitogen-activated protein kinases independently of MyD88, and cause dendritic cells to mature [1].
TLR3-DN is a TIR-less form of the TLR3 gene generated by deleting the TIR domain (450 bp). This truncated gene is still able to recognize its ligands but is unable to induce the signaling cascade.

1. Alexopoulou L. et al. (2001). Nature 413(6857):732-8.
2. Matsumoto M. et al. (2002). Biochem Biophys Res Commun. 293(5):1364-9.
3.Takeuchi O.et al. (2001). Int Immunol, 13(7):933-40.

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Specifications

Human TLR3-DN (pUNO1-hTLR03-DN)

ORF size : 2265 bp
Subclone : AgeI - NheI

Mouse TLR3-DN (pUNO1-mTLR03-DN)

ORF size : 2268 bp
Subclone : AgeI - NheI

Human TLR3-DN-HA (pUNO1-hTLR03-DN-HA)

ORF size : 2358 bp
Subclone : AgeI - NheI

Mouse TLR3-DN-HA (pUNO1-mTLR03-DN-HA)

Subclone : AgeI - NheI

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Contents pUNO1

  • 20 µg of lyophilized DNA
  • 2 x 1 ml blasticidin at 10 mg/ml

 

room temperature Product is shipped at room temperature.

store Lyophilized DNA should be stored at -20 ̊C.

stability Resuspended DNA should be stored at -20 ̊C and is stable up to 1 year.

Alert Blasticidin is a harmful compound. Refer to safety data sheet for handling instructions.

 

Store blasticidin at 4°C or -20°C for up to two years. Product is stable 2 weeks at 37°C.

Avoid repeated freeze-thaw cycles.

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Citations

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