Invivogen
Menu

ASC

pUNO1-hASCa Unit size Cat. code Docs Qty Price
pUNO1 bearing the human ASC gene, isoform 1 (long)
20 µg
puno1-hasca
+-
$384.00
pUNO1-hASC-DN Unit size Cat. code Docs Qty Price
pUNO1 bearing a dominant negative version of the human ASC gene
20 µg
puno1-hasc-dn
+-
$406.00
pUNO1-mASC Unit size Cat. code Docs Qty Price
pUNO1 bearing the mouse ASC gene
20 µg
puno1-masc
+-
$384.00
pUNO1-mASC-DN Unit size Cat. code Docs Qty Price
pUNO1 bearing a dominant negative version of the mouse ASC gene
20 µg
puno1-masc-dn
+-
$406.00

Expression-ready ORF Clones

Alias: PYCARD, CARD5, TMS1

 

Apoptosis-associated speck-like protein containing a CARD.
PYD and CARD domain containing.

 

Available versions:

  • Wild type ASC
  • Dominant negative ASC (see the "Details" tab)
Back to the top

Specifications

Human ASC isoform 1 (long) (pUNO1-hASCa)

Genbank : NM_013258.4
ORF size : 588 bp
Subclone : AgeI - NheI

Human ASC-DN (pUNO1-hASC-DN)

ORF size : 285 bp
Subclone : AgeI - NheI

Mouse ASC (pUNO1-mASC)

Genbank : NM_023258.4
ORF size : 582 bp
Subclone : NcoI - NheI

Mouse ASC-DN (pUNO1-mASC-DN)

ORF size : 282 bp
Subclone : NcoI - NheI

Back to the top

Contents pUNO1

  • 20 µg of lyophilized DNA
  • 2 x 1 ml blasticidin at 10 mg/ml

 

room temperature Product is shipped at room temperature.

store Lyophilized DNA should be stored at -20 ̊C.

stability Resuspended DNA should be stored at -20 ̊C and is stable up to 1 year.

Alert Blasticidin is a harmful compound. Refer to safety data sheet for handling instructions.

 

Store blasticidin at 4°C or -20°C for up to two years. Product is stable 2 weeks at 37°C.

Avoid repeated freeze-thaw cycles.

Back to the top

Details

ASC-DN version

The dominant negative version of ASC is a truncated form of the gene (aa1-94) that contains the Pyrin domain but not the CARD domain. ASC-DN binds to NALP1 but not caspase-1 and strongly blocks maturation of IL-1β in PMA-differentiated THP1 cells stimulated with LPS without affecting LPS-induced proIL-1β synthesis [1].

1. Martinon F et al., 2002. The Inflammasome: A Molecular Platform Triggering Activation of Inflammatory Caspases and Processing of proIL-1β. Mol Cell (10): 417-426.

Back to the top
Customer Service
& Technical Support
Shopping cart is empty