ASC
| pUNO1-hASCa | Unit size | Cat. code | Docs | Qty | Price |
|---|---|---|---|---|---|
pUNO1 bearing the human ASC gene, isoform 1 (long) |
20 µg |
puno1-hasca |
| pUNO1-hASC-DN | Unit size | Cat. code | Docs | Qty | Price |
|---|---|---|---|---|---|
pUNO1 bearing a dominant negative version of the human ASC gene |
20 µg |
puno1-hasc-dn |
| pUNO1-mASC | Unit size | Cat. code | Docs | Qty | Price |
|---|---|---|---|---|---|
pUNO1 bearing the mouse ASC gene |
20 µg |
puno1-masc |
| pUNO1-mASC-DN | Unit size | Cat. code | Docs | Qty | Price |
|---|---|---|---|---|---|
pUNO1 bearing a dominant negative version of the mouse ASC gene |
20 µg |
puno1-masc-dn |
Expression-ready ORF Clones
Alias: PYCARD, CARD5, TMS1
Apoptosis-associated speck-like protein containing a CARD.
PYD and CARD domain containing.
Available versions:
- Wild type ASC
- Dominant negative ASC (see the "Details" tab)
Specifications
Human ASC isoform 1 (long) (pUNO1-hASCa)
Genbank : NM_013258.4
ORF size : 588 bp
Subclone : AgeI - NheI
Human ASC-DN (pUNO1-hASC-DN)
ORF size : 285 bp
Subclone : AgeI - NheI
Mouse ASC (pUNO1-mASC)
Genbank : NM_023258.4
ORF size : 582 bp
Subclone : NcoI - NheI
Mouse ASC-DN (pUNO1-mASC-DN)
ORF size : 282 bp
Subclone : NcoI - NheI
pUNO1 Contents
- 20 µg of lyophilized DNA
- 2 x 1 ml blasticidin at 10 mg/ml
Product is shipped at room temperature.
Lyophilized DNA should be stored at -20°C. Upon receipt, store blasticidin at 4°C or -20°C.
Resuspended DNA should be stored at -20°C and is stable for up to 1 year.
Blasticidin is a harmful compound. Refer to the safety data sheet for handling instructions.
Details
ASC-DN version
The dominant negative version of ASC is a truncated form of the gene (aa1-94) that contains the Pyrin domain but not the CARD domain. ASC-DN binds to NALP1 but not caspase-1 and strongly blocks maturation of IL-1β in PMA-differentiated THP1 cells stimulated with LPS without affecting LPS-induced proIL-1β synthesis [1].
1. Martinon F et al., 2002. The Inflammasome: A Molecular Platform Triggering Activation of Inflammatory Caspases and Processing of proIL-1β. Mol Cell (10): 417-426.
Back to the top