CpG-free hph

Synthetic hygromycin resistance

ABOUT

Synthetic hygromycin resistance gene in cloning plasmid

Resistance to the antibiotic hygromycin is conferred by the hph gene encoding a hygromycin B phosphotransferase isolated from an E. coli strain.

The DNA sequence of this CpG-free gene was modified by optimizing the codon usage and deleting the CpG motifs without changing the amino acid sequence of the wild type protein.

The synthetic hph gene is provided in the bacterial plasmid pMOD2 which is selectable with Ampicillin.

To facilitate the excision and subcloning of the synthetic hph gene into another vector, this gene is flanked by a unique restriction site at the 5’ and 3’ end.

All products are for research use only, and not for human or veterinary use.

SPECIFICATIONS

Specifications

Gene
CpG-free hph (hygromycin B phosphotransferase)
ORF size
3107 bp
Plasmid backbone
pMOD2
Antibiotic resistance
Ampicillin
Tested applications

in vitro transfection

Quality control

Plasmid construct is confirmed by restriction analysis and full-length open reading frame (ORF) sequencing.

CONTENTS

Contents

  • Product: 
    pMOD2-Hygro
  • Cat code: 
    pmod2-hygro
  • Quantity: 
    20 µg

Shipping & Storage

  • Shipping method:  Room temperature

    • Storage:

    • -20°C

DOCUMENTS

Documents

pMOD2-Hygro

Technical Data Sheet

Plasmid Sequence

Safety Data Sheet

Certificate of analysis

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