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pDUO2-MD2/CD14

pDUO2-hMD2/CD14 Unit size Cat. code Docs Qty Price
pDUO2 bearing human MD2/CD14 Gene
20 µg
pduo2-hmd2cd14
+-
$480.00
pDUO2-mMD2/CD14 Unit size Cat. code Docs Qty Price
pDUO2 bearing mouse MD2/CD14 Gene
20 µg
pduo2-mmd2cd14
+-
$480.00

pDUO2-MD2/CD14 is an expression vector designed to co-express MD2 and CD14 genes known to interact with each other : human MD2 (480 bp) and human CD14 (1125 bp) or mouse MD2 (483 bp) and mouse CD14 (1101 bp).

The genes cloned into pDUO2 comprise the coding sequence (without introns) from the ATG to the Stop codon.

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Specifications

pDUO2 plasmid with human MD2 and human CD14 genes.
Name: MD2 / CD14
Species:
Human
Backbone: pDUO2
Selection: Hygromycin

pDUO2 plasmid with murine MD2 and murine CD14 genes.
Name: MD2 / CD14
Species: Mouse
Backbone: pDUO2
Selection: Hygromycin

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Contents

- 20 µg of lyophilized DNA
- 4 pouches of E. coli Fast-Media® Hygro (2 TB and 2 Agar)

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Description

MD2 and CD14 are necessary for proper LPS-induced TLR4 signaling.
TLR4 is the receptor for Gram-negative lipopolysaccharide (LPS). TLR4 alone is not sufficient to confer LPS responsiveness. MD-2 is a secreted molecule that functionally interacts with LPS [1,2]. TLR4 physically associates with MD2 and CD14 to form the complex responsible for LPS recognition and signaling [3].

1. Shimazu R. et al., 1999. MD-2, a molecule that confers lipopolysaccharide responsiveness on Toll-like receptor 4. J Exp Med, 189(11):1777-82
2. Nagai Y. et al., 2002. Essential role of MD-2 in LPS responsiveness and TLR4 distribution. Nat Immunol. 3(7):667-72
3. da Silva Correia J. et al., 2001. Lipopolysaccharide is in close proximity to each of the proteins in its membrane receptor complex. transfer from CD14 to TLR4 and MD-2. J Biol Chem. 276(24):21129-35

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Details

pDUO backbone

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Citations

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