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pUNO1 bearing the human STING gene, I200N variant
STING (stimulator of interferon genes; also known as TMEM173, MITA, MPYS, and ERIS) is essential for the IFN response to microbial or self-DNA, and acts as a direct sensor of cyclic dinucleotides (CDNs).
CDNs are important messengers in bacteria, affecting numerous responses of the prokaryotic cell, but also in mammalian cells, acting as agonists of the innate immune response.
Studies have revealed that STING variation can affect CDN recognition and signal transduction.
The hSTING-N200 isoform harbors a missense mutation (I200N) equivalent to I199N mutation found in the Goldenticket (Gt) mouse strain [1, 2]. Residue I200 is buried in the interior of the STING protomer.
The I200N mutation results in a null-phenotype with no detectable STING activity .
1. Yin Q. et al., 2012. Cyclic di-GMP sensing via the innate immune signaling protein STING. Mol Cell 46(6):735-45.
2. Sauer JD. et al., 2011. The N-ethyl-N-nitrosourea-induced Goldenticket mouse mutant reveals an essential function of Sting in the in vivo interferon response to Listeria monocytogenes and cyclic dinucleotides. Infect Immun 79(2):688-94.
Human STING-N200 (pUNO1-hSTING-N200)
ORF size : 1140 bp
Subclone : BspEI - NheI