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pUNO1 bearing the A162 isoform human STING (S162A) gene
STING (stimulator of interferon genes; also known as TMEM173, MITA, MPYS, and ERIS) is essential for the IFN response to microbial or self-DNA, and acts as a direct sensor of cyclic dinucleotides (CDNs).
CDNs are important messengers in bacteria, affecting numerous responses of the prokaryotic cell, but also in mammalian cells, acting as agonists of the innate immune response.
Human wild-type STING fails to bind DMXAA, a potent tumor vascular disrupting agent in mice . The allele A162 contains a unique point mutation (S162A) placed at the cyclic-dinucleotide-binding site which confers DMXAA sensitivity to hSTING .
1. Conlon J. et al., 2013. Mouse, but not human STING, binds and signals in response to the vascular disrupting agent 5,6-dimethylxanthenone-4-acetic acid. J Immunol 190(10):5216-25.
2. Gao P. et al., 2013. Structure-function analysis of STING activation by c[G(2',5')pA(3',5')p] and targeting by antiviral DMXAA. Cell 154(4):748-62.
Human STING-A162 (pUNO1-hSTING-A162)
ORF size : 1140 bp
Subclone : BspEI - NheI