Anti-HER2-Tra-hIgA2

Monoclonal human IgA2 antibody against HER2

Anti-HER2-Tra-hIgA2 features the constant region of the human IgA2 isotype and the variable region of trastuzumab. Trastuzumab is a humanized IgG1 monoclonal antibody that targets the HER2 receptor (human epidermal growth factor receptor 2 also known as HER2/neu) that is found on the cell membrane of epithelial cells. HER2 plays an important role in normal cell growth and differentiation [1]. However, in certain types of cancers, particularly in breast and ovarian cancers, HER2 is over-expressed and causes uncontrollable cell proliferation. Binding of trastuzumab to HER2 results in cell death through different mechanisms including antibody-dependent cell-mediated cytotoxicity and phagocytosis [2, 3]. Trastuzumab has been approved by the FDA for the treatment of breast cancer.
Anti-HER2-Tra-hIgA2 was generated by recombinant DNA technology. It has been produced in CHO cells and purified by affinity chromatography with peptide M.

 

References:

1. Rubin I. & Yarden Y. 2001. The basic biology of HER2. Ann Oncol.12 Suppl 1:S3-8.
2. Collins DM. et al., 2012. Trastuzumab induces antibody-dependent cell-mediated cytotoxicity (ADCC) in HER-2-non-amplified breast cancer cell lines. Ann Oncol. 23(7):1788-95.
3. Petricevic B. et al., 2013. Trastuzumab mediates antibody-dependent cell- mediated cytotoxicity and phagocytosis to the same extent in both adjuvant and metastatic HER2/neu breast cancer patients. J Transl Med. 11:307.

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Specifications

Specificity: Targets cells expressing HER2

Clonality: Monoclonal antibody

Isotype: Human IgA2, kappa

Source: CHO cells

Purity: Purified by affinity chromatography with peptide M

Quality control:

• Binding of Anti-HER2-Tra-hIgG1 to HER2 has been tested using flow cytometry.
• The complete sequence of this antibody has been verified.
• The absence of bacterial contamination (e.g. lipoproteins and endotoxins) has been confirmed using HEK-Blue™ TLR2 and HEK-Blue™ TLR4 cells.

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Contents

anti-HER2-Tra-hIgA2 antibody is provided azide-free and lyophilized.

• 100 µg purified anti-HER2-Tra-hIgA2 antibody

Product is shipped at room temperature

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