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Human ACE2 & TMPRSS2 & NLRP1 & ASC-GFP Reporter A549 Cell Lines

Two cell lines (ACE2-OE & TMPRSS2-OE w/o NLRP1-OE) - ASC-GFP speck forming lung carcinoma

Overexpression of TMPRSS2 (qRT-PCR)

Cell surface expression of ACE2 (flow-cytometry)

NLRP1 and ASC-GFP expression (Western blot)

ASC speck formation upon NLRP1 inflammasome activation (with hTNF-α stimulation)

ASC speck formation upon NLRP1 inflammasome activation (without hTNF-α stimulation)

Pyroptotic cell death upon NLRP1 inflammasome activation

Human ACE2 & TMPRSS2 A549 Cell Lines

Two cell lines (ACE2-OE w/o TMPRSS2-OE) - Lung carcinoma

Validation of ACE2 overexpression by RT-qPCR

Validation of ACE2 surface expression on A549-hACE2 cells by FACS

Infection of A549-hACE2 cells by SARS-CoV-2 Spike pseudotyped lentiviral particles

Validation of ACE2 and TMPRSS2 overexpression by RT-qPCR

Validation of ACE2 surface expression on A549-hACE2-TMPRSS2 cells by FACS

Infection of A549-hACE2-TMPRSS2 cells by SARS-CoV-2 Spike pseudotyped lentiviral particles