hLC3b-GFP fusion gene
mLC3b-GFP fusion gene
pSELECT-GFP-LC3 are mammalian expression vectors containing the human or murine LC3B gene fused at its 5’ end to the green fluorescent protein (GFP) gene.
The same plasmid is available with the GFP gene alone as a control. This control plasmid is called pSELECT-NGFP-zeo.
Expression of the GFP-LC3 fusion gene allows to visualize autophagosome formation in real time in live cells.Back to the top
Gene: GFP::LC3B fusion gene
Promoter: hEF1-HTLVBack to the top
- 20 µg of lyophilized DNA
- 1 ml of Zeocin™ (100 mg/ml)
Product is shipped at room temperatureBack to the top
pSELECT plasmids are specifically designed for strong and constitutive expression of a gene of interest in a wide variety of cell lines. They allow the selection of stable transfectants and offer a variety of selectable markers. pSELECT plasmids contain two expression cassettes: the first drives the expression of the gene of interest and the second drives the expression of a large choice of dominant selectable markers for both E. coli and mammalian cells.
pSELECT-GFP-LC3 are mammalian expression vector containing the human or murine LC3B gene fused at its 5’ end to the green fluorescent protein (GFP) gene. This plasmid is selectable in bacteria and mammalian cells using Zeocin™. Expression of the GFP-LC3B fusion gene allows to visualize autophagosome formation in real time in live cells. During autophagosome formation, GFP-LC3B is processed and recruited to the autophagosome membrane, where it can be imaged as cytoplasmic puncta by high resolution fluorescence microscopy. The percentage of GFP-LC3B positive cells can be determined and is indicative of autophagosome formation.
GFP::LC3B fusion gene was generated by fusing a GFP variant to the 5’ end of the human LC3B gene. A synthetic intron was added between both moieties to increase the activity of GFP. This hybrid protein absorbs blue light (major peak at 480 nm) and emits green light (major peak at 505 nm).
Plasmid construct has been confirmed by restriction analysis and sequencing.
Plasmid DNA was purified by ion exchange chromatography and lyophilized.
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