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LC3B-GFP

pSELECT-GFP-hLC3 Unit size Cat. code Docs Qty Price
hLC3b-GFP fusion gene
20 µg
psetz-gfplc3
+-
$433.00
pSELECT-GFP-mLC3 Unit size Cat. code Docs Qty Price
mLC3b-GFP fusion gene
20 µg
psetz-gfpmlc3
+-
$433.00

pSELECT-GFP-LC3 are mammalian expression vectors containing the human or murine LC3B gene fused at its 5’ end to the green fluorescent protein (GFP) gene.
The same plasmid is available with the GFP gene alone as a control. This control plasmid is called pSELECT-NGFP-zeo.

Expression of the GFP-LC3 fusion gene allows to visualize autophagosome formation in real time in live cells.

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Specifications

Selection: Zeocin™

Gene: GFP::LC3B fusion gene

Promoter: hEF1-HTLV

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Contents

- 20 µg of pSELECT-GFP-hLC3 plasmid provided as lyophilized DNA
- 4 pouches of E. coli Fast-Media® Zeo (2 TB and 2 Agar)

or

- 20 μg of pSELECT-GFP-mLC3 plasmid provided as lyophilized DNA
- 4 pouches of E. coli Fast-Media® Zeo (2 TB and 2 Agar)

Product is shipped at room temperature.

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Description

pSELECT plasmids are specifically designed for strong and constitutive expression of a gene of interest in a wide variety of cell lines. They allow the selection of stable transfectants and offer a variety of selectable markers. pSELECT plasmids contain two expression cassettes: the first drives the expression of the gene of interest and the second drives the expression of a large choice of dominant selectable markers for both E. coli and mammalian cells.

pSELECT-GFP-LC3 are mammalian expression vector containing the human or murine LC3B gene fused at its 5’ end to the green fluorescent protein (GFP) gene. This plasmid is selectable in bacteria and mammalian cells using Zeocin™. Expression of the GFP-LC3B fusion gene allows to visualize autophagosome formation in real time in live cells. During autophagosome formation, GFP-LC3B is processed and recruited to the autophagosome membrane, where it can be imaged as cytoplasmic puncta by high resolution fluorescence microscopy. The percentage of GFP-LC3B positive  cells can be determined and is indicative of autophagosome formation.

GFP::LC3B fusion gene was generated by fusing a GFP variant to the 5’ end of the human LC3B gene. A synthetic intron was added between both moieties to increase the activity of GFP. This hybrid protein absorbs blue light (major peak at 480 nm) and emits green light (major peak at 505 nm).

Plasmid construct has been confirmed by restriction analysis and sequencing.
Plasmid DNA was purified by ion exchange chromatography and lyophilized.

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Details

pSELECT-GFP-LC3

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Citations

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