Anti-HLA Class I Control

Monoclonal IgG1 positive control for class I Luminex® (LABScreen® or LIFECODES HLA)

IgG1 are the most abundant IgG present in serum (66%). They have a high complement-activating capacity and important cellular effector function.

Anti-HLA Class I Control is a chimeric human IgG1 monoclonal antibody designed for studying detection of human IgG antibodies to HLA class I. Its constant regions are from human IgG1 (i.e. human IgG1 heavy chains and kappa light chains) and its variable regions are derived from the murine monoclonal anti-HLA class I W6/32 [1, 2].

Anti-HLA Class I Control can be used as an IgG positive control for IgG anti-HLA antibody detection assays, including LABScreen®, LIFECODES HLA and C1qScreen™.

Anti-HLA Class I Control has been validated on LABScreen® Mixed and LABScreen® Single Antigen HLA Class I. It reacts with all class I HLA-A, B and Cw antigens [3]. The anti-HLA Class I IgG1 Control binds strongly to recombinant C1q using the C1qScreen™ kit (see figures).

References:

1. Barnstable CJ. et al., 1978. Production of monoclonal antibodies to group A erythrocytes, HLA and other human cell surface antigens-new tools for genetic analysis. Cell. 14(1):9-20.
2. Hilton HG, Parham P., 2013. Direct binding to antigen-coated beads refines the specificity and cross-reactivity of four monoclonal antibodies that recognize polymorphic epitopes of HLA class I molecules. Tissue Antigens. 81(4):212-20
3. Congy-Jolivet N et al. 2013. Production and characterization of chimeric anti-HLA monoclonal antibodies targeting public epitopes as tools for standardizations of the anti-HLA antibody detection. J Immunol Methods. 390(1-2):41-51