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Human TLR Dual Reporter HEK293 Cells (NF-κB and IRF)

NF-κB–SEAP and IRF–Lucia reporter HEK293 cells expressing Toll-like receptors

Signaling pathways in HEK-Dual™ TLR cells
Signaling pathways in HEK-Dual™ TLR cells
(click to enlarge and see legend)

HEK-Dual™ TLR cells are a collection of the human embryonic kidney 293 (HEK293)-derived cell lines designed to provide a rapid, sensitive, and reliable method to screen and validate Toll-like receptor (TLR) agonists or antagonists. 

These cells express two inducible reporter genes for SEAP (secreted embryonic alkaline phosphatase) and Lucia luciferase. As a result, these cells allow the simultaneous study of the NF-κB and IRF pathways by assessing the activity of the SEAP and the secreted Lucia luciferase activity, respectively. Both reporter proteins are readily measurable in the cell culture supernatant when using QUANTI-Blue™, a SEAP detection reagent, and QUANTI-Luc™ 4 Lucia/Gaussia, a Lucia luciferase detection reagent.

InvivoGen's cell lines are extensively tested for viability, stability, biological activity, and absence of mycoplasma to ensure strong and reproducible results. Moreover, we provide detailed handling and experimental procedures for all cell lines, to minimize the need for optimization or troubleshooting by the end-user.

Key features:

  • Stable expression of human TLR
  • Strong response to their specific ligands
  • Distinct monitoring of TLR-dependent NF-κB and IRF activation
  • Functionally tested and mycoplasma-free

Applications:

  • Defining the role of TLR-dependent IRF and NF-κB signaling pathways
  • Screening for novel TLR agonists and inhibitors


Toll-like Receptors (TLRs) were the first pattern recognition receptors (PRRs) identified in mammals and are the best characterized to date. They initiate key inflammatory responses and also shape adaptative immunity. They recognize diverse pathogen-associated molecular patterns (PAMPs) from bacteria, fungi, parasites, and viruses, including lipid-based bacterial cell wall components such as lipopolysaccharide (LPS) and lipopeptides, microbial protein components such as flagellin, and nucleic acids such as single-stranded or double-stranded RNA and CpG DNA. InvivoGen also provides a growing library of TLR ligands.

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