Reporter Detection Kits
Gene reporter systems are extensively used for the study of eukaryotic gene expression and cellular events coupled to gene expression. Typically the reporter gene of choice is cloned with a promoter sequence of interest into an expression vector that is then tranferred into cells. Cells are subsequently assayed for the presence of the reporter by directly measuring the reporter protein itself or the enzymatic activity of the reporter protein, either in vitro or in vivo.
An ideal reporter system has no background activity and quantitative measurements are made using assays that are easy, sensitive and reliable.
InvivoGen provides three reporter gene systems:
• Secreted embryonic alkaline phosphatase (SEAP) reporter gene system
SEAP is readily quantified by measuring the enzyme activity in the supernatant of cultured cells (like InvivoGen's Reporter Cells) using the colorimetric assays, QUANTI-Blue™ and HEK-Blue™ Detection.
• Secreted synthetic coelenterazine luciferase Lucia reporter gene system
Lucia luciferase can be directly measured in the cell culture supernatant using the innovative and ready-to-use bioluminescent assay reagent QUANTI-Luc™.
• β-Galactosidase (LacZ) reporter gene system