InvivoGen provides an expanding collection of recombinant cell lines in which specific genes are over-expressed or have been knocked out. These cell lines are useful for:
- studying gene function;
- investigating cell signaling pathways and signal transduction;
- screening and validating agonists/antagonists of pattern recognition receptors (PRRs), such as Toll-like receptors (TLRs), C-type lectin receptors (CLRs) and cytosolic DNA sensors (CDSs);
- detecting cytokines; and
- validating antibody function.
Most of InvivoGen's cell lines stably express an inducible secreted reporter gene encoding either SEAP (secreted embryonic alkaline phosphatase) or Lucia luciferase. Among these reporter cells, some are knocked-out for a specific gene.
All of InvivoGen’s cell lines have been extensively validated. Each batch is tested for viability, biological activity and absence of mycoplasma to ensure valid, reproducible results and to minimize the need for any optimization or troubleshooting by the end-user. Furthermore, all cell lines come supplied with detailed protocols that include instructions for general cell culture and maintenance, as well as sample experimental set-ups.