Custom Cloning

Many Combinations Possible According to Your Aims

At the leading edge of research and development in vector construction, gene delivery, and gene expression systems, InvivoGen specialists provide an extensive range of customized products. With more than 20 years of expertise in cloning, InvivoGen specialists offer a choice of custom cloning services at very competitive rates and short turn-around time.

Services

- Custom-made pSELECT : Gene subcloning

- Custom-made pDRIVE : Promoter assembly and subcloning

- Custom-made psiRNA : shRNA design and cloning

 

Description

• Gene subcloning : Custom-made pSELECT
InvivoGen offers a large collection of genes provided in pUNO1, an expression plasmid selectable with Blasticidin. If the use of this antibiotic does not suit your needs, our experts can subclone any of our genes into a pSELECT plasmid carrying the selectable marker of your choice : hygromycin, G418/Geneticin, puromycin, Zeocin or GFP::Zeocin.

• Promoter assembly and subcloning : Custom-made pDRIVE
You can create your own composite promoter by combining the enhancer, core promoter and 5’UTR chosen from InvivoGen’s list (See Custom-Made pDRIVE List tab) . We will assemble this composite promoter in a pDRIVE plasmid harboring a reporter gene, either SEAP, Lucia luciferase or GFP or a gene chosen from our collection. We can also replace the selection marker.

• shRNA design and cloning : Custom-made psiRNA plasmid
You can either send us your siRNA/shRNA sequence or we can select the best candidate siRNA sequence for your target gene using the siRNA Wizard software.

The insert encoding the expected shRNA sequence will be cloned in the psiRNA-h7SK plasmid which is available with different selectable markers : blasticidin, G418/Geneticin, Zeocin or GFP::Zeocin.

Specifications

• Wide expertise
• Customized scientific support
• Rapid processing
• Ready-to-use custom-made product
• Cost-effective

Contents

Custom-made pSELECT, pDRIVE or psiRNA plasmids are provided as 20 µg of lyophilized DNA.
Products are shipped at room temperature.
Store at -20°C.

Custom-Made pDRIVE List

ENHANCERS
Enhancer Name Origin Specificity
AldA Aldolase A Human Ubiquitous
AFP Alpha-Fetoprotein Human AFP-positive cells
CEA Carcinoembryonic antigen Human CEA-positive cells
hCMV Human Cytomegalovirus Viral Ubiquitous
mCMV Mouse Cytomegalovirus Viral Ubiquitous
P2 Adipocyte P2 Mouse Adipose tissue
PSA Prostate specific antigen Human PSA-positive cells
SV40 Simian virus Viral Ubiquitous
Tie2 Angiopoietin receptor Mouse Vascular endothelial cells
Tyr Tyrosinase Mouse Melanocytes and melanoma
CORE PROMOTERS
Ubiquitous Promoter Name Origin Specificity
Beta-Act Beta-Actin Human Ubiquitous
hCMV Human Cytomegalovirus Viral Ubiquitous
hEF-1alpha Elongation Factor 1 Human Ubiquitous
rEF-1alpha Elongation Factor 1 Rat Ubiquitous
chEF-1alpha Elongation Factor 1 Chimpanzee Ubiquitous
FerH Ferritin H Human Ubiquitous
FerL Ferritin L Human Ubiquitous
Tissue-Specific Promoter Name Origin Specificity
bAlb Albumin Bovin Liver
hAlb Albumin Human Liver
CD43 Leukosialin Human Leukocytes and platelets
CD45 Leukocyte Common Antigen Human Hematopoietic cells
NSE Neuron-Specific Enolase Rat Mature neurons
Tumor-Specific Promoter Name Origin Specificity
AFP Alpha-Fetoprotein Human Hepatocellular carcinoma
CEA Carcinoembryonic Antigen Human Epithelial cancers
PSA Prostate Specific Antigen Human Prostate cancers
Tyr Tyrosinase Mouse Melanoma
5' UNTRANSLATED REGIONS
5' UTR Name Origin Activity
AdTp Adenotripartite Viral Enhances translation
mEF-1alpha Elongation Factor 1 Mouse Increases mRNA translation
chEF-1alpha Elongation Factor 1 Chimpanzee Increases mRNA translation
eIF4g Eukaryotic initiation factor 4g Human Contains a putative IRES
HSP70 Heat shock protein 70 Human Increases mRNA translation
NRF NF-κB repressing factor Mouse Acts as a potent IRES
HTLV RU5’ Human T-cell leukemia virus Viral Increases mRNA translation

ORDERING

Custom-made pSELECT

Description Gene subcloning
Cat. Codep-custom-select
Unit Size20 ug
Price For price or distributor address,
please select your country

    Custom-made pDRIVE

    Description Promoter assembly and subcloning
    Cat. Codep-custom-drive
    Unit Size20 ug
    Price For price or distributor address,
    please select your country

      Custom-made psiRNA

      Description shRNA design and cloning
      Cat. Codep-custom-sirna
      Unit Size20 ug
      Price For price or distributor address,
      please select your country

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        Disclaimer: Our products are provided for research purpose only. Commercial applications may require licensing from third parties.
        Note that the sequence of available ORFs provided by InvivoGen can differ from a given reference Genbank record due to genetic variations and/or alternative splicing. Customers should verify that the version of a gene sold by InvivoGen is suitable for the customer needs.
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