Many Combinations Possible According to Your Aims
At the leading edge of research and development in vector construction, gene delivery, and gene expression systems, our specialists are pleased to provide an extensive range of customized products. With more than 20 years of experience in cloning, we offer custom cloning services at very competitive rates and short turn-around time.
• Wide Expertise and Consultation Available
• Customized Scientific Support
• Rapid Processing
• Your Product Ready-to-Use
pORF and pMOD plasmids are not selectable in mammalian cells. If you are planning on stably expressing an open reading frame provided in a pORF or pMOD, our experts can subclone this open reading frame into a pSELECT plasmid carrying the selectable marker of your choice:
You can create your own composite promoter by combining the enhancer, core promoter and 5’UTR of your choice. We will assemble this composite promoter in a pDRIVE plasmid harboring a reporter gene from the list below:
Custom-made pDRIVE carry the Zeocin™ resistance gene for selection and amplification in E. coli. We can replace the bacterial selection cassette with a mammalian selection cassette of your choice derived from a pSELECT plasmid.
You can either send us your siRNA/shRNA sequence or we can select the best candidate siRNA sequence for your target gene using the siRNA Wizard software. The insert encoding the expected shRNA sequence will be cloned in the psiRNA-h7SK plasmid which is available with different selectable markers:
Contents and Storage
Custom-made plasmids (pSELECT, pDRIVE or psiRNA) are provided as 20 µg of lyophilized DNA.
Custom-made psiRNA plasmids are also available as a kit that contains 20 µg of custom-made psiRNA, 20 µg of a control psiRNA, 1 vial of LyoComp GT116 (E. coli competent cells), and 4 pouches of the corresponding Fast-Media® (E. coli selection medium).
Products are shipped at room temperature. Store at -20°C.
|Custom-made pSELECT ||20 µg||contact us|
|Custom-made pDRIVE ||20 µg||contact us|
|Custom-made psiRNA ||20 µg||contact us|
|Custom-made psiRNA kit ||1 kit||contact us|