Zeocin™
InvivoGen is the sole worldwide producer of Zeocin™, a copper-chelated glycopeptide antibiotic produced by Streptomyces CL990.
Zeocin™ causes cell death by intercalating into DNA and cleaving it. This antibiotic is effective on most aerobic cells and is therefore useful for selection in bacteria, eukaryotic microorganisms, plant and animal cells. Resistance to Zeocin™ is conferred by the Sh ble gene product which inactivates Zeocin™ by binding to the antibiotic.
Zeocin™ is used at a concentration of 50-300 µg/ml for selection in mammalian cells and 25 µg/ml for bacterial selection.
Specifications
Working concentration:
- 50-300 µg/ml in mammalian cells
- 25 µg/ml in bacteria
CAS number: 11006-33-0
Purity: >90%
Molecular weight: 1525
Formula: C55H85O21N20S2Cu - HCl
Documents
- TDS Zeocin™ : 1 g (ant-zn-1) , 5 g (25 x 2 ml) (ant-zn-5) , 5 g (50 ml bottle) (ant-zn-5b)
- TDS Zeocin™ : 1 g (powder) (ant-zn-1p) , 5 g (powder) (ant-zn-5p)
- MSDS Zeocin™ : 1 g (ant-zn-1) , 5 g (25 x 2 ml) (ant-zn-5) , 5 g (50 ml bottle) (ant-zn-5b)
- MSDS Zeocin™ : 1 g (powder) (ant-zn-1p) , 5 g (powder) (ant-zn-5p)
Contents
Zeocin™ is supplied as a 100 mg/ml solution in HEPES buffer or as a blue powder in different formats:
- 5 x 2 ml at 100 mg/ml (1 g)
- 25 x 2 ml at 100 mg/ml (5 g)
- 1 x 50 ml at 100 mg/ml (5 g)
- 1 x 1 g tube (powder)
- 1 x 5 g bottle (powder)
Ref.
- DROCOURT, D., T. CALMELS, J.P. REYNES, M. BARON and G. TIRABY. 1990.Cassettes of the Streptoalloteichus hindustanus ble gene for transformation of lower and higher eukaryotes to phleomycin resistance. Nucl. Acids. Res. 18: 4009
- GATIGNOL,A., DURAND, H. and G. TIRABY. 1988. Bleomycin resistance conferred by a drug-binding protein. FEBS Letters. 230 : 171-175
- DUMAS, Ph., M. BERGDOLL, C. CAGNON and J.M. MASSON. 1994. The threedimensional structure of a bleomycin resistance protein. Embo J. 242 (5) 595-601
- LI, F., WILKINS, P.P., CRAWLEY, S., WEINSTEIN, J. CUMMINGS, R.D. and R.P. McEVER. 1996. Post-translational modifications of recombinant P-selection glycoprotein ligand-1 required for binding to P- and E- selection. J. Biol. Chem. 271 : 3255-3264
- FENG,Y., BRODER, C.C., KENNEDY, P.E. and E.A. BERGER. 1996. HIV-1 entry cofactor: functional c DNA cloning of a seven-transmembrane, G-protein-coupled receptor. Science. 272 : 872-877
- APT, K.E., KROTH-PANCIC, P.G. and A.R. GROSSMAN. 1996. Stable nuclear transformation of the diatom Phaeodactylium tricornutum. Mol. Gen. Genet. 252 : 572-579
- BOUAYADI, K., HOFFMANN J.S., FONS, P., TIRABY,M., REYNES, J.P. and C. CAZAUX. 1997. Overexpression of DNApolymerase beta sensitizesmammalian cells to 2',3' deoxycytidine and 3'-azido-3'-deoxythymidine. Cancer Res. 57 : 110-116
- PFEIFER, T.A., HEGEDUS, D.D., GRIGLIATTI, T.A. and D.A. THEILMAN. 1997. Baculovirus immediate-early promoter-mediated expression of the Zeocin resistance gene for use as a dominant selectable marker in Dipteran and Lepidopteran insect cell lines. Gene. 188 : 183-190
- BAGNIS, C., GRAVIS, G., IMBERT,A.M., HERRERA, D.,ALLARIO, .T, GALINDO, R., LOPEZ, M., PAVON, C., SEMPERE, C., and P. MANNONI. 1994. Retroviral transfer of the nlsLacZ gene into human CD34+ cell populations
ORDERING
Zeocin™
| Cat. Code | ant-zn-1 |
|---|---|
| Unit Size | 1 g |
| Price | For price or distributor address, please select your country |
Zeocin™
| Cat. Code | ant-zn-5 |
|---|---|
| Unit Size | 5 g (25 x 2 ml) |
| Price | For price or distributor address, please select your country |
Zeocin™
| Cat. Code | ant-zn-5b |
|---|---|
| Unit Size | 5 g (50 ml bottle) |
| Price | For price or distributor address, please select your country |
Zeocin™
| Cat. Code | ant-zn-1p |
|---|---|
| Unit Size | 1 g (powder) |
| Price | For price or distributor address, please select your country |
Zeocin™
| Cat. Code | ant-zn-5p |
|---|---|
| Unit Size | 5 g (powder) |
| Price | For price or distributor address, please select your country |
