Zeocin™

Selection antibiotic: cell culture tested, sterile reagent

InvivoGen is the sole worldwide producer of Zeocin™, a popular and effective antibiotic for the selection of vectors bearing the Sh ble gene in a variety of cells types (bacteria, eukaryotic microorganisms, plant and animal cells).

Resistance to Zeocin™ is conferred by the Sh ble gene product, which inactivates Zeocin™ by binding to the antibiotic. Sh ble is a small gene, only 370bp in size, carried in a number of InvivoGen’s vectors.

Zeocin™ is a copper-chelated glycopeptide antibiotic produced by Streptomyces CL990. Zeocin™ causes cell death by intercalating into DNA and cleaving it. The action of Zeocin™ is effective on most aerobic cells.

Typically, mammalian cells are sensitive to Zeocin™ concentrations of 50-400 µg/ml, and bacteria to 25 µg/ml.

Specifications

Product concentration: 100 mg/ml in solution
CAS number: 11006-33-0

Quality Control: Each lot is thoroughly tested to ensure the absence of lot-to-lot variation.
Endotoxin level:
< 1 EU/mg
Physicochemical characterization:
HPLC, pH, appearance
Cell-culture tested:
potency validated in Zeocin™-sensitive and Zeocin™-resistant mammalian cell lines
Non-cytotoxicity of trace contaminants:
absence of long-term effects confirmed in Zeocin™-resistant cells

Contents

Zeocin™ is supplied as a sterile filtered blue solution at 100 mg/ml in HEPES buffer. This product is available in three pack sizes:
- ant-zn-1: 10 x 1 ml (1 g)
- ant-zn-5: 50 x 1 ml (5 g)
- ant-zn-5b: 1 x 50 ml (5 g)

Zeocin™ is also supplied as a blue powder:
- ant-zn-1p: 1 x 1 g
- ant-zn-5p: 1 x 5 g

Zeocin™ is shipped at room temperature. Upon receipt it should be stored at 4°C or -20°C.

Citations

Recent articles using InvivoGen Zeocin™

ORDERING

Zeocin™

Cat. Code ant-zn-1
Unit Size 1g (10 x 1 ml)
Price For price or distributor address,
please select your country

Zeocin™

Cat. Code ant-zn-5
Unit Size 5g (50 x 1 ml)
Price For price or distributor address,
please select your country

Zeocin™

Cat. Code ant-zn-5b
Unit Size 5 g (50 ml bottle)
Price For price or distributor address,
please select your country

Zeocin™

Cat. Code ant-zn-5p
Unit Size 5 g (powder)
Price For price or distributor address,
please select your country

Zeocin™

Cat. Code ant-zn-1p
Unit Size 1 g (powder)
Price For price or distributor address,
please select your country
Disclaimer: Our products are provided for research purposes only. Not for use in humans. Commercial applications may require licensing from third parties.
Note that the sequence of available ORFs provided by InvivoGen can differ from a given reference Genbank record due to genetic variations and/or alternative splicing. Customers should verify that the version of a gene sold by InvivoGen is suitable for the customer needs.
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