Zeocin™

Selection antibiotic: cell culture tested, sterile reagent

InvivoGen is the sole worldwide producer of Zeocin™, a popular and effective antibiotic for the selection of vectors bearing the Sh ble gene in a variety of cells types (bacteria, eukaryotic microorganisms, plant and animal cells).

Resistance to Zeocin™ is conferred by the Sh ble gene product, which inactivates Zeocin™ by binding to the antibiotic. Sh ble is a small gene, only 370bp in size, carried in a number of InvivoGen’s vectors.

Zeocin™ is a copper-chelated glycopeptide antibiotic produced by Streptomyces CL990. Zeocin™ causes cell death by intercalating into DNA and cleaving it. The action of Zeocin™ is effective on most aerobic cells.

Typically, mammalian cells are sensitive to Zeocin™ concentrations of 50-400 µg/ml, and bacteria to 25 µg/ml.

Specifications

Product concentration: 100 mg/ml in solution
CAS number: 11006-33-0

Quality Control: Each lot is thoroughly tested to ensure the absence of lot-to-lot variation.
Endotoxin level:
< 1 EU/mg
Physicochemical characterization:
HPLC, pH, appearance
Cell-culture tested:
potency validated in Zeocin™-sensitive and Zeocin™-resistant mammalian cell lines
Non-cytotoxicity of trace contaminants:
absence of long-term effects confirmed in Zeocin™-resistant cells

Contents

Zeocin™ is supplied as a sterile filtered blue solution at 100 mg/ml in HEPES buffer. This product is available in three pack sizes:
- ant-zn-1: 10 x 1 ml (1 g)
- ant-zn-5: 50 x 1 ml (5 g)
- ant-zn-5b: 1 x 50 ml (5 g)

Zeocin™ is also supplied as a blue powder:
- ant-zn-1p: 1 x 1 g
- ant-zn-5p: 1 x 5 g

Zeocin™ is shipped at room temperature. Upon receipt it should be stored at 4°C or -20°C.

Citations

Recent articles using InvivoGen Zeocin™

ORDERING

Zeocin™

Cat. Code ant-zn-1
Unit Size 1g (10 x 1 ml)
Price For price or distributor address,
please select your country

Zeocin™

Cat. Code ant-zn-5
Unit Size 5g (50 x 1 ml)
Price For price or distributor address,
please select your country

Zeocin™

Cat. Code ant-zn-5b
Unit Size 5 g (50 ml bottle)
Price For price or distributor address,
please select your country

Zeocin™

Cat. Code ant-zn-5p
Unit Size 5 g (powder)
Price For price or distributor address,
please select your country

Zeocin™

Cat. Code ant-zn-1p
Unit Size 1 g (powder)
Price For price or distributor address,
please select your country
Disclaimer: Our products are provided for research purpose only. Commercial applications may require licensing from third parties.
Note that the sequence of available ORFs provided by InvivoGen can differ from a given reference Genbank record due to genetic variations and/or alternative splicing. Customers should verify that the version of a gene sold by InvivoGen is suitable for the customer needs.
Copyrights © 2011-2015 InvivoGen. All Rights Reserved. Reproduction of any materials from this site is strictly forbidden without permission for commercial use. Nonprofit use for non-commercial research and educational purposes is permitted, citation should include the URL "www.invivogen.com".