Specifications

Working concentration:
- 50-300 µg/ml in mammalian cells
- 25 µg/ml in bacteria
CAS number: 11006-33-0
Purity: controlled by HPLC >90%
Molecular weight: 1525
Formula: C₅₅H₈₅O₂₁N₂₀S₂Cu - HCl
Quality control
Zeocin™ in solution in HEPES buffer, pH 7.25, is filtered to sterility for customer convenience, and validated for cell-culture usage.
Activity controlled by microbiological assays

Documents

• TDS Zeocin™ : 1g (10 x 1 ml) (ant-zn-1) , 5g (50 x 1 ml) (ant-zn-5) , 5 g (50 ml bottle) (ant-zn-5b)
• TDS Zeocin™ : 1 g (powder) (ant-zn-1p) , 5 g (powder) (ant-zn-5p)
• MSDS Zeocin™ : 1g (10 x 1 ml) (ant-zn-1) , 5g (50 x 1 ml) (ant-zn-5) , 5 g (50 ml bottle) (ant-zn-5b)
• MSDS Zeocin™ : 1 g (powder) (ant-zn-1p) , 5 g (powder) (ant-zn-5p)

Contents

Zeocin™ is supplied as either a blue powder or a 100 mg/ml solution (100% active product) in HEPES buffer in 1 ml tubes or a 50 ml bottle:
- ant-zn-1: 10 x 1 ml at 100 mg/ml (1 g)
- ant-zn-5: 50 x 1 ml at 100 mg/ml (5 g)
- ant-zn-5b: 1 x 50 ml at 100 mg/ml (5 g)
- ant-zn-1p: 1 x 1 g tube (powder)
- ant-zn-5p: 1 x 5 g bottle (powder)

Zeocin™ is shipped at room temperature. Upon receipt it should be stored at 4°C or -20°C.

Ref.

- DROCOURT, D., T. CALMELS, J.P. REYNES, M. BARON and G. TIRABY. 1990.Cassettes of the Streptoalloteichus hindustanus ble gene for transformation of lower and higher eukaryotes to phleomycin resistance. Nucl. Acids. Res. 18: 4009
- GATIGNOL,A., DURAND, H. and G. TIRABY. 1988. Bleomycin resistance conferred by a drug-binding protein. FEBS Letters. 230 : 171-175
- DUMAS, Ph., M. BERGDOLL, C. CAGNON and J.M. MASSON. 1994. The threedimensional structure of a bleomycin resistance protein. Embo J. 242 (5) 595-601
- LI, F., WILKINS, P.P., CRAWLEY, S., WEINSTEIN, J. CUMMINGS, R.D. and R.P. McEVER. 1996. Post-translational modifications of recombinant P-selection glycoprotein ligand-1 required for binding to P- and E- selection. J. Biol. Chem. 271 : 3255-3264
- FENG,Y., BRODER, C.C., KENNEDY, P.E. and E.A. BERGER. 1996. HIV-1 entry cofactor: functional c DNA cloning of a seven-transmembrane, G-protein-coupled receptor. Science. 272 : 872-877
- APT, K.E., KROTH-PANCIC, P.G. and A.R. GROSSMAN. 1996. Stable nuclear transformation of the diatom Phaeodactylium tricornutum. Mol. Gen. Genet. 252 : 572-579
- BOUAYADI, K., HOFFMANN J.S., FONS, P., TIRABY,M., REYNES, J.P. and C. CAZAUX. 1997. Overexpression of DNApolymerase beta sensitizesmammalian cells to 2',3' deoxycytidine and 3'-azido-3'-deoxythymidine. Cancer Res. 57 : 110-116
- PFEIFER, T.A., HEGEDUS, D.D., GRIGLIATTI, T.A. and D.A. THEILMAN. 1997. Baculovirus immediate-early promoter-mediated expression of the Zeocin resistance gene for use as a dominant selectable marker in Dipteran and Lepidopteran insect cell lines. Gene. 188 : 183-190
- BAGNIS, C., GRAVIS, G., IMBERT,A.M., HERRERA, D.,ALLARIO, .T, GALINDO, R., LOPEZ, M., PAVON, C., SEMPERE, C., and P. MANNONI. 1994. Retroviral transfer of the nlsLacZ gene into human CD34+ cell populations

Citations

Recent articles using Zeocin™

• 2011 - PNAS, 108: 16663 - 16668
  CD147 subunit of lactate/H+ symporters MCT1 and hypoxia-inducible MCT4 is critical for energetics and growth of glycolytic tumors.
  Le Floch R, Chiche J, Marchiq I, Naiken T, Ilk K, Murray CM, Critchlow SE, Roux D, Simon MP, Pouyssegur J
  Free article
• 2011 - Infect. Immun., 79: 1418 - 1427
  NOD-like receptor activation by outer membrane vesicles from Vibrio cholerae non-O1 non-O139 strains is modulated by the quorum-sensing regulator HapR.
  Bielig H, Rompikuntal PK, Dongre M, Zurek B, Lindmark B, Ramstedt M, Wai SN, Kufer TA
  Free article
• 2012 - PNAS, 109: 78 - 83
  Combinatorial reshaping of the Candida antarctica lipase A substrate pocket for enantioselectivity using an extremely condensed library.
  Sandstrom AG, Wikmark Y, Engstrom K, Nyhlen J, Backvall JE
  Free article