Zeocin™

InvivoGen is the sole worldwide producer of Zeocin™, a popular and effective antibiotic for the selection of vectors bearing the Sh ble gene in a variety of cells types (bacteria, eukaryotic microorganisms, plant and animal cells).

Resistance to Zeocin™ is conferred by the Sh ble gene product, which inactivates Zeocin™ by binding to the antibiotic. Sh ble is a small gene, only 370bp in size, carried in a number of InvivoGen’s vectors.

Zeocin™ is a copper-chelated glycopeptide antibiotic produced by Streptomyces CL990. Zeocin™ causes cell death by intercalating into DNA and cleaving it. The action of Zeocin™ is effective on most aerobic cells.

Specifications

Product concentration: 100 mg/ml in solution
Working concentration:
- 50-400 µg/ml in mammalian cells
- 25 µg/ml in bacteria
CAS number: 11006-33-0
Purity:
controlled by HPLC >90%
Molecular weight:
1525
Formula: C55H85O21N20S2Cu - HCl
Quality control
Zeocin™ in solution in HEPES buffer, pH 7.25, is filtered to sterility for customer convenience, and validated for cell-culture usage.
Activity controlled by microbiological assays

Contents

Zeocin™ is supplied as either a blue powder or a 100 mg/ml solution (100% active product) in HEPES buffer in 1 ml tubes or a 50 ml bottle:
- ant-zn-1: 10 x 1 ml at 100 mg/ml (1 g)
- ant-zn-5: 50 x 1 ml at 100 mg/ml (5 g)
- ant-zn-5b: 1 x 50 ml at 100 mg/ml (5 g)
- ant-zn-1p: 1 x 1 g tube (powder)
- ant-zn-5p: 1 x 5 g bottle (powder)

Zeocin™ is shipped at room temperature. Upon receipt it should be stored at 4°C or -20°C.

 

Ref.

- DROCOURT, D., T. CALMELS, J.P. REYNES, M. BARON and G. TIRABY. 1990.Cassettes of the Streptoalloteichus hindustanus ble gene for transformation of lower and higher eukaryotes to phleomycin resistance. Nucl. Acids. Res. 18: 4009
- GATIGNOL,A., DURAND, H. and G. TIRABY. 1988. Bleomycin resistance conferred by a drug-binding protein. FEBS Letters. 230 : 171-175
- DUMAS, Ph., M. BERGDOLL, C. CAGNON and J.M. MASSON. 1994. The threedimensional structure of a bleomycin resistance protein. Embo J. 242 (5) 595-601
- LI, F., WILKINS, P.P., CRAWLEY, S., WEINSTEIN, J. CUMMINGS, R.D. and R.P. McEVER. 1996. Post-translational modifications of recombinant P-selection glycoprotein ligand-1 required for binding to P- and E- selection. J. Biol. Chem. 271 : 3255-3264
- FENG,Y., BRODER, C.C., KENNEDY, P.E. and E.A. BERGER. 1996. HIV-1 entry cofactor: functional c DNA cloning of a seven-transmembrane, G-protein-coupled receptor. Science. 272 : 872-877
- APT, K.E., KROTH-PANCIC, P.G. and A.R. GROSSMAN. 1996. Stable nuclear transformation of the diatom Phaeodactylium tricornutum. Mol. Gen. Genet. 252 : 572-579
- BOUAYADI, K., HOFFMANN J.S., FONS, P., TIRABY,M., REYNES, J.P. and C. CAZAUX. 1997. Overexpression of DNApolymerase beta sensitizesmammalian cells to 2',3' deoxycytidine and 3'-azido-3'-deoxythymidine. Cancer Res. 57 : 110-116
- PFEIFER, T.A., HEGEDUS, D.D., GRIGLIATTI, T.A. and D.A. THEILMAN. 1997. Baculovirus immediate-early promoter-mediated expression of the Zeocin resistance gene for use as a dominant selectable marker in Dipteran and Lepidopteran insect cell lines. Gene. 188 : 183-190
- BAGNIS, C., GRAVIS, G., IMBERT,A.M., HERRERA, D.,ALLARIO, .T, GALINDO, R., LOPEZ, M., PAVON, C., SEMPERE, C., and P. MANNONI. 1994. Retroviral transfer of the nlsLacZ gene into human CD34+ cell populations

Citations

Recent articles using Zeocin™

ORDERING

Zeocin™

Cat. Code ant-zn-1
Unit Size 1g (10 x 1 ml)
Price For price or distributor address,
please select your country

Zeocin™

Cat. Code ant-zn-5
Unit Size 5g (50 x 1 ml)
Price For price or distributor address,
please select your country

Zeocin™

Cat. Code ant-zn-5b
Unit Size 5 g (50 ml bottle)
Price For price or distributor address,
please select your country

Zeocin™

Cat. Code ant-zn-1p
Unit Size 1 g (powder)
Price For price or distributor address,
please select your country

Zeocin™

Cat. Code ant-zn-5p
Unit Size 5 g (powder)
Price For price or distributor address,
please select your country
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Note that the sequence of available ORFs provided by InvivoGen can differ from a given reference Genbank record due to genetic variations and/or alternative splicing. Customers should verify that the version of a gene sold by InvivoGen is suitable for the customer needs.
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