Hygromycin B
Hygromycin B is an aminoglycoside antibiotic produced by Streptomyces hygroscopicus. It inhibits protein synthesis by interfering with translocation and causing mistranslation at the 70S ribosome. Hygromycin B is effective on most bacteria, fungi and higher eukaryotes.
Resistance to hygromycin is conferred by the hph gene from E. coli.
Hygromycin B is normally used at a concentration of 50-200 µg/ml in mammalian cells and 100 µg/ml in bacteria.
InvivoGen also provides HygroGold™, an ultra-pure preparation of hygromycin B.
Specifications
Working concentration:
- 50-200 µg/ml in mammalian cells
-100 µg/ml in bacteria
CAS number: 31282-04-9
Purity: >85%
Molecular weight: 527.52
Formula: C20H37N3O13, HCl
Docs
Contents
Hygromycin B is supplied as a yellow solution in HEPES buffer in different formats:
- 5 x 2 ml at 100 mg/ml (1 g)
- 1 x 50 ml at 100 mg/ml (5 g)
Details
Ref.
- Cabanas, M.et al. (1978) Eur. J. Biochem. 87, 21-27
- Gonzales, A. et al. (1978) Biochem. Biophys. Acta 521, 459-469
- Singh, A. et al. (1979) Nature 277, 146-148
- Gritz, L. & Davies, J. (1983) Gene 25, 179-188
- Cullen, D. et al. (1987) Gene 57, 21-26
- Sugden, B. et al. (1985) Mol. Cell. Biol. 4, 2929-2931
- Santerre, R.F. et al. Gene 30, 147-156
- Hemmi, S., et al. (1992) Proc. Natl. Acad. Sci. USA 89, 2737-2741
- Mac Gregor, G.R. et al (1987) Somatic Cell. Mol. Genet. 13, 253-265
Citations
Recent articles using Hygromycin B
- 2012 - J. Biol. Chem., 287: 5008 - 5020
Cys-27 variant of human {delta}-opioid receptor modulates maturation and cell surface delivery of Phe-27 variant via heteromerization.
Leskela TT, Lackman JJ, Vierimaa MM, Kobayashi H, Bouvier M, Petaja-Repo UE. - 2012 - Nucleic Acids Res., 40(9):4097-109
C7orf30 is necessary for biogenesis of the large subunit of the mitochondrial ribosome.
Rorbach J, Gammage PA, Minczuk M
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ORDERING
Hygromycin B
| Cat. Code | ant-hm-1 |
|---|---|
| Unit Size | 1 g (5 x 2 ml) |
| Price | For price or distributor address, please select your country |
Hygromycin B
| Cat. Code | ant-hm-5 |
|---|---|
| Unit Size | 5 g (1 x 50 ml) |
| Price | For price or distributor address, please select your country |