Hygromycin B

Hygromycin B is an aminoglycoside antibiotic produced by Streptomyces hygroscopicus.It inhibits protein synthesis by interfering with translocation and causing mistranslation at the 70S ribosome. Hygromycin B is effective on most bacteria, fungi and higher eukaryotes.
Resistance to hygromycin is conferred by the hph gene from E. coli.

Hygromycin B is normally used at a concentration of 50-200 µg/ml in mammalian cells and 100 µg/ml in bacteria.

InvivoGen also provides HygroGold™, an ultra-pure preparation of hygromycin B.

Product change notification

April, 30th 2014 - Important note about InvivoGen’s Hygromycin B products.
Starting October 27th, 2014 InvivoGen will sell only one Hygromycin B product. InvivoGen’s high purity HygroGold™ will be renamed Hygromycin B Gold and the standard Hygromycin B will be discontinued.
A protocol will be provided to customers currently working with Hygromycin B that will facilitate the transition from using Hygromycin B to Hygromycin B Gold.


Product concentration: 100 mg/ml
Working concentration:
- 50-500 µg/ml in mammalian cells
- 50-100 µg/ml in bacteria
CAS number
: 31282-04-9
Molecular weight:
Formula: C20H37N3O13, HCl


Hygromycin B is supplied as a yellow solution in HEPES buffer in different formats:

-  10 x 1 ml at 100 mg/ml (1 g)

-  1 x 50 ml at 100 mg/ml (5 g)


hygromycin B


- Cabanas, M.et al. (1978) Eur. J. Biochem. 87, 21-27
- Gonzales, A. et al. (1978) Biochem. Biophys. Acta 521, 459-469
- Singh, A. et al. (1979) Nature 277, 146-148
- Gritz, L. & Davies, J. (1983) Gene 25, 179-188
- Cullen, D. et al. (1987) Gene 57, 21-26
- Sugden, B. et al. (1985) Mol. Cell. Biol. 4, 2929-2931
- Santerre, R.F. et al. Gene 30, 147-156
- Hemmi, S., et al. (1992) Proc. Natl. Acad. Sci. USA 89, 2737-2741
- Mac Gregor, G.R. et al (1987) Somatic Cell. Mol. Genet. 13, 253-265


Recent articles using InvivoGen Hygromycin B


Hygromycin B

Cat. Code ant-hm-1
Unit Size 1g (10 x 1 ml)
Price For price or distributor address,
please select your country

Hygromycin B

Cat. Code ant-hm-5
Unit Size 5 g (1 x 50 ml)
Price For price or distributor address,
please select your country
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Note that the sequence of available ORFs provided by InvivoGen can differ from a given reference Genbank record due to genetic variations and/or alternative splicing. Customers should verify that the version of a gene sold by InvivoGen is suitable for the customer needs.
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