Hygromycin B Gold

Selection antibiotic: cell culture tested, sterile reagent

Hygromycin B | InvivoGen

Hygromycin B Gold™ is an ultrapure Hygromycin B.
Hygromycin B is an aminoglycoside antibiotic produced by Streptomyces hygroscopicus. It kills bacteria, fungi and higher eukaryotic cells by inhibiting protein synthesis. It has been reported to interfere with translocation [1] and to cause mistranslation at the 70S ribosome [2].

Resistance to Hygromycin B

Hygromycin B is used as a selective agent in molecular genetics experiments on a wide variety of eukaryotic and prokaryotic species [3-5].
The hph gene confers hygromycin-resistance to cells expressing it and many vectors carrying the hph gene are available within the scientific community. Typically, mammalian cells are sensitive to Hygromycin B concentrations of 50-200 µg/ml, and bacteria to 50-100 µg/ml.

Protocol to facilitate the transition from using Hygromycin B to Hygromycin B Gold.


Product concentration: 100 mg/ml solution
CAS number: 31282-04-9

Quality Control: Each lot is thoroughly tested to ensure the absence of lot-to-lot variation.
Purity: ≥ 90% (HPLC)
Endotoxin level: < 0.5 EU/mg
Physicochemical characterization: pH, appearance
Cell culture tested: potency validated in hygromycin‑sensitive and hygromycin-resistant mammalian cell lines
Non‑cytotoxicity of trace contaminants: absence of long‑term effects confirmed in hygromycin‑resistant cells


Hygromycin B Gold (previously named HygroGold™) is supplied as a sterile filtered yellow solution at 100 mg/ml in HEPES buffer. This product is available in two pack sizes:
- ant-hg-1: 10 x 1 ml (1 g)
- ant-hg-5: 1 x 50 ml (5 g)

Hygromycin B Gold is shipped at room temperature. Upon receipt, it should be stored at 4°C or -20˚C.

Hygromycin B is a hazardous compound. Avoid contact with skin and eyes.


The working concentrations of Hygromycin B Gold for mammalian cell lines vary from 50 to 200 μg/ml, in a few cases up to 500 μg/ml.
If you notice unusually high cytotoxicity when switching to Hygromycin B Gold, you may need to adjust your working concentrations.
Suggested concentrations of Hygromycin B Gold for selection in some examples of mammalian cells are listed below.

Cell line Species Tissue Medium Hygromycin B Equivalent concentrations
Hygromycin B
(ant-hm-1, ant‑hm-5)
Hygromycin B Gold
(ant-hg-1, ant-hg-5)
B16  Mouse Melanoma   RPMI  100-200 µg/ml  200 µg/ml  160 µg/ml
CHO  Hamster  Ovary  MEM  100-500 µg/ml  500 µg/ml  400 µg/ml
HeLa  Human  Cervix  DMEM  100-200 µg/ml  200 µg/ml  160 µg/ml
HEK293  Human Kidney  DMEM  50-400 µg/ml  500 µg/ml  320 µg/ml
Raji  Human  Lymphoma  RPMI  125-300 µg/ml  300 µg/ml  240 µg/ml
THP-1  Human  Monocytes  RPMI  200-400 µg/ml  500 µg/ml  320 µg/ml


1. Cabanas M. et al., 1978. Dual interference of Hygromycin B with ribosomal translocation and with Aminoacyl-tRNA recognition. Eur. J. Biochem. 87:21‑7.
2. Gonzales, A. et al., 1978. Studies on the mode of action of hygromycin B, an inhibitor of translocation in eukaryotes. Biochem Biophys Acta 521:459‑69.
3. Gritz L. & Davies J., 1983. Plasmid-encoded hygromycin B resistance: the sequence of hygromycin B phosphotransferase gene and its expression in Escherichia coli and Saccharomyces cerevisiae. Gene 25:179-88.
4. Cullen D. et al., 1987. Transformation of Aspergillus nidulans with the hygromycin-resistance gene, hph. Gene 57:21-6.
5. Santerre R. et al., 1984. Expression of prokaryotic genes for hygromycin B and G418 resistance as dominant-selection markers in mouse L cells. Gene 30:147-56.


Recent articles using InvivoGen Hygromycin B Gold


Hygromycin B Gold (solution)

Description Selective antibiotic for the hph gene
Cat. Codeant-hg-1
Unit Size1 g (10 x 1 ml)
Price For price or distributor address,
please select your country

Hygromycin B Gold (solution)

Description Selective antibiotic for the hph gene
Cat. Codeant-hg-5
Unit Size5 g (1 x 50 ml)
Price For price or distributor address,
please select your country
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