Hygromycin B Gold

High-purity selective antibiotic for the hph gene

Hygromycin B Gold™ is an ultrapure Hygromycin B.
Hygromycin B is an aminoglycoside antibiotic produced by Streptomyces hygroscopicus. It kills bacteria, fungi and higher eukaryotic cells by inhibiting protein synthesis. It has been reported to interfere with translocation [1] and to cause mistranslation at the 70S ribosome [2].

Resistance to Hygromycin B

Hygromycin B is used as a selective agent in molecular genetics experiments on a wide variety of eukaryotic and prokaryotic species [3-5].
The hph gene confers hygromycin-resistance to cells expressing it and many vectors carrying the hph gene are available within the scientific community.

Protocol to facilitate the transition from using Hygromycin B to Hygromycin B Gold.

Product change notification

October, 27th 2014 - InvivoGen now only sells one Hygromycin B product. InvivoGen’s high purity HygroGold™ is renamed Hygromycin B Gold and the standard Hygromycin B is discontinued.

Specifications

Product concentration: 100 mg/ml solution
Working concentration:
- 50-500 µg/ml in mammalian cells
- 50-500 µg/ml in bacteria
CAS number
: 31282-04-9
Purity:
>90% (confirmed by HPLC)
Activity is tested using bioassays on bacteria and mammalian cell lines.
Molecular weight:
563.5
pKa values: 7.1 and 8.8 in water.
Formula: C20H37N3O13, HCl

Contents

Hygromycin B Gold (previously named HygroGold™) is supplied as a yellow solution in HEPES buffer or as a white lyophilized powder in different formats:

-  1g : 10 x 1 ml at 100 mg/ml

-  5g: 1 x 50 ml at 100 mg/ml

-  10 g (powder)

Hygromycin B Gold is shipped at room temperature. Upon receipt, it should be stored at 4°C or -20˚C.

SPECIAL HANDLING
Hygromycin B is a hazardous compound. Avoid contact with skin and eyes.

Description

The working concentrations of Hygromycin B Gold for mammalian cell lines vary from 50 to 200 μg/ml, in a few cases up to 500 μg/ml.
If you notice unusually high cytotoxicity when switching to Hygromycin B Gold, you may need to adjust your working concentrations.
Suggested concentrations of Hygromycin B Gold for selection in some examples of mammalian cells are listed below.

Cell line Species Tissue Medium Hygromycin B Equivalent concentrations
Hygromycin B
(ant-hm-1, ant‑hm-5)
Hygromycin B Gold
(ant-hg-1, ant-hg-5)
B16  Mouse Melanoma   RPMI  100-200 µg/ml  200 µg/ml  160 µg/ml
CHO  Hamster  Ovary  MEM  100-500 µg/ml  500 µg/ml  400 µg/ml
HeLa  Human  Cervix  DMEM  100-200 µg/ml  200 µg/ml  160 µg/ml
HEK293  Human Kidney  DMEM  50-400 µg/ml  500 µg/ml  320 µg/ml
Raji  Human  Lymphoma  RPMI  125-300 µg/ml  300 µg/ml  240 µg/ml
THP-1  Human  Monocytes  RPMI  200-400 µg/ml  500 µg/ml  320 µg/ml

Ref.

1. Cabanas M. et al., 1978. Dual interference of Hygromycin B with ribosomal translocation and with Aminoacyl-tRNA recognition. Eur. J. Biochem. 87:21‑7.
2. Gonzales, A. et al., 1978. Studies on the mode of action of hygromycin B, an inhibitor of translocation in eukaryotes. Biochem Biophys Acta 521:459‑69.
3. Gritz L. & Davies J., 1983. Plasmid-encoded hygromycin B resistance: the sequence of hygromycin B phosphotransferase gene and its expression in Escherichia coli and Saccharomyces cerevisiae. Gene 25:179-88.
4. Cullen D. et al., 1987. Transformation of Aspergillus nidulans with the hygromycin-resistance gene, hph. Gene 57:21-6.
5. Santerre R. et al., 1984. Expression of prokaryotic genes for hygromycin B and G418 resistance as dominant-selection markers in mouse L cells. Gene 30:147-56.

Citations

Recent articles using InvivoGen Hygromycin B Gold

ORDERING

Hygromycin B Gold (solution)

Description Selective antibiotic for the hph gene
Cat. Code ant-hg-1
Unit Size 1 g (10 x 1 ml)
Price For price or distributor address,
please select your country

Hygromycin B Gold (solution)

Description Selective antibiotic for the hph gene
Cat. Code ant-hg-5
Unit Size 5 g (1 x 50 ml)
Price For price or distributor address,
please select your country

Hygromycin B Gold (powder)

Description Selective antibiotic for the hph gene
Cat. Code ant-hg-10p
Unit Size 10 g
Price For price or distributor address,
please select your country
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