Selection antibiotic: cell culture tested, sterile reagent
G418, also known as G418 sulfate and Geneticin, is an aminoglycoside antibiotic similar in structure to gentamicin B1, produced by Micromonospora rhodorangea. G418 blocks polypeptide synthesis by inhibiting the elongation step in both prokaryotic and eukaryotic cells. Resistance to G418 is conferred by the Neomycin resistance gene (neo) from Tn5 encoding an aminoglycoside 3‘-phosphotransferase, APH 3‘ II.
Selection in mammalian cells is usually achieved in three to seven days with concentrations ranging from 400 to 1000 µg/ml. Cells that are dividing are affected sooner than those that are not.
Product concentration: 100 mg/ml
CAS number: 108321-42-2
Quality Control: Each lot is thoroughly tested to ensure the absence of lot-to-lot variation.
Purity: ≥ 90% (HPLC)
Endotoxin level: < 0.5 EU/mg
Physicochemical characterization: pH, appearance
Cell-culture tested: potency validated in G418-sensitive and G418-resistant mammalian cell lines
Non-cytotoxicity of trace contaminants: absence of long-term effects confirmed in G418-resistant cells
G418 is supplied as a sterile filtered solution at 100 mg/ml in HEPES buffer. This product is available in two pack sizes:
- ant-gn-1: 10 x 1 ml (1 g)
- ant-gn-5: 1 x 50 ml (5 g)
G418 is shipped at room temperature. Upon receipt it should be stored at 4°C.
Recent articles using InvivoGen G418 (Geneticin)
- 2017 - Nat Commun., 8:13958.
Cannabinoid CB2 receptor ligand profiling reveals biased signalling and off-target activity.
Soethoudt M. et al.
- 2017 - Nat Cell Biol., 19(2):120-132.
Phenotypic heterogeneity of disseminated tumour cells is preset by primary tumour hypoxic microenvironments.
Fluegen G. et al.
- 2017 - PLoS One., 12(2):e0172405.
Structure, kinetic characterization and subcellular localization of the two ribulose 5-phosphate epimerase isoenzymes from Trypanosoma cruzi.
Gonzalez SN. et al.
- 2016 - PNAS, [Epub ahead of print]
Spatial dynamics of SIRT1 and the subnuclear distribution of NADH species.
Aguilar-Arnal L. et al.
- 2016 - Science., 353(6305).
Targeted nucleotide editing using hybrid prokaryotic and vertebrate adaptive immune systems.
Nishida K. et al.
|Description||Selective antibiotic for the neo gene|
|Unit Size||1 g (10 x 1ml)|
|Price||For price or distributor address,
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