Selection antibiotic: cell culture tested, sterile reagent
G418, also known as G418 sulfate and Geneticin, is an aminoglycoside antibiotic similar in structure to gentamicin B1, produced by Micromonospora rhodorangea. G418 blocks polypeptide synthesis by inhibiting the elongation step in both prokaryotic and eukaryotic cells. Resistance to G418 is conferred by the Neomycin resistance gene (neo) from Tn5 encoding an aminoglycoside 3‘-phosphotransferase, APH 3‘ II.
Selection in mammalian cells is usually achieved in three to seven days with concentrations ranging from 400 to 1000 µg/ml. Cells that are dividing are affected sooner than those that are not.
Product concentration: 100 mg/ml
CAS number: 108321-42-2
Quality Control: Each lot is thoroughly tested to ensure the absence of lot-to-lot variation.
Purity: ≥ 90% (HPLC)
Endotoxin level: < 0.5 EU/mg
Physicochemical characterization: pH, appearance
Cell-culture tested: potency validated in G418-sensitive and G418-resistant mammalian cell lines
Non-cytotoxicity of trace contaminants: absence of long-term effects confirmed in G418-resistant cells
G418 is supplied as a sterile filtered solution at 100 mg/ml in HEPES buffer. This product is available in two pack sizes:
- ant-gn-1: 10 x 1 ml (1 g)
- ant-gn-5: 1 x 50 ml (5 g)
G418 is shipped at room temperature. Upon receipt it should be stored at 4°C.
Recent articles using InvivoGen G418 (Geneticin)
- 2016 - Science., 353(6305).
Targeted nucleotide editing using hybrid prokaryotic and vertebrate adaptive immune systems.
Nishida K. et al.
- 2016 - Nucleic Acids Res., 44(10):4763-84.
Diverged composition and regulation of the Trypanosoma brucei origin recognition complex that mediates DNA replication initiation.
Marques CA. et al.
- 2016 - Stem Cells Dev., [Epub ahead of print]
Id2 mediates differentiation of labyrinthine placental progenitor cell line, SM10.
Selesniemi KL, Albers RE, Brown TL.
- 2016 - Dev Comp Immunol., 54(1):75-88.
Structurally diverse genes encode Tlr2 in rainbow trout: The conserved receptor cannot be stimulated by classical ligands to activate NF-κB in vitro.
Brietzke A, Arnemo M, Gjøen T, Rebl H, Korytář T, Goldammer T, Rebl A, Seyfert HM.
- 2015 - J Virol., 89(5):2956-61.
Virion-independent transfer of replication-competent hepatitis C virus RNA between permissive cells.
Longatti A, Boyd B, Chisari FV.
|Unit Size||1 g (10 x 1ml)|
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