G418

G418 is an aminoglycoside antibiotic similar in structure to gentamicin B1, produced by Micromonospora rhodorangea. G418 blocks polypeptide synthesis by inhibiting the elongation step in both prokaryotic and eukaryotic cells. Resistance to G418 is conferred by the Neomycin resistance gene (neo) from Tn5 encoding an aminoglycoside 3‘-phosphotransferase, APH 3‘ II.

Selection in mammalian cells is usually achieved in three to seven days with concentrations ranging from 400 to 1000 µg/ml. Cells that are dividing are affected sooner than those that are not.

Specifications

Product concentration: 100 mg/ml

Working concentration: 400 to 1000 µg/ml in mammalian cells

CAS number: 108321-42-2

Molecular weight: 692.7

Formula: C20H40N4O10. 2H2SO4

Contents

G418 Sulfate is supplied as a 100 mg/ml solution in H2O in different formats:

-  10 x 1 ml at 100 mg/ml (1 g)

-  1 x 50 ml at 100 mg/ml (5 g)

Details

G418 structure

Ref.

- Davies J & Jimenez A. 1980. A new selective agent for eukaryotic cloning vectors. Am J Trop Med Hyg 29(5 Suppl):1089-92
- Bar-Nun S et al. 1983. G-418, an elongation inhibitor of 80 S ribosomes. Biochim Biophys Acta. 741(1):123-7.
- Beck E et al. 1982. Nucleotide sequence and exact localization of the neomycin phosphotransferase gene from transposon Tn5. Gene 19(3):327-36

Citations

Recent articles using InvivoGen G418

ORDERING

G418

Cat. Code ant-gn-1
Unit Size 1 g (10 x 1ml)
Price For price or distributor address,
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G418

Cat. Code ant-gn-5
Unit Size 5 g (1 x 50 ml)
Price For price or distributor address,
please select your country
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