G418 is an aminoglycoside antibiotic similar in structure to gentamicin B1, produced by Micromonospora rhodorangea. G418 blocks polypeptide synthesis by inhibiting the elongation step in both prokaryotic and eukaryotic cells. Resistance to G418 is conferred by the Neomycin resistance gene (neo) from Tn5 encoding an aminoglycoside 3‘-phosphotransferase, APH 3‘ II.
Selection in mammalian cells is usually achieved in three to seven days with concentrations ranging from 400 to 1000 µg/ml. Cells that are dividing are affected sooner than those that are not.
Working concentration: 400 to 1000 µg/ml in mammalian cells
CAS number: 108321-42-2
Molecular weight: 692.7
Formula: C20H40N4O10. 2H2SO4
G418 Sulfate is supplied as a 100 mg/ml solution in H2O in different formats:
- 5 x 2 ml at 100 mg/ml (1 g)
- 1 x 50 ml at 100 mg/ml (5 g)
- Davies J & Jimenez A. 1980. A new selective agent for eukaryotic cloning vectors. Am J Trop Med Hyg 29(5 Suppl):1089-92
- Bar-Nun S et al. 1983. G-418, an elongation inhibitor of 80 S ribosomes. Biochim Biophys Acta. 741(1):123-7.
- Beck E et al. 1982. Nucleotide sequence and exact localization of the neomycin phosphotransferase gene from transposon Tn5. Gene 19(3):327-36
Recent articles using G418
- 2011 - J Biol Chem., 286: 38795 - 38804
Toll-like receptor 3 (TLR3) signaling requires TLR4 Interactor with leucine-rich REPeats (TRIL).
Carpenter S, Wochal P, Dunne A, O'Neill LA
- 2011 - J.Virol., 85: 9767 - 9777
Infection of lymphoblastoid cell lines by Kaposi's sarcoma-associated herpesvirus: critical role of cell-associated virus.
Myoung J, Ganem D.
|Unit Size||1 g (5 x 2 ml)|
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