Phleomycin

Phleomycin is a glycopeptide antibiotic of the bleomycin family, isolated from a mutant strain of Streptomyces verticillus. It binds and intercalates DNA thus destroying the integrity of the double helix. Phleomycin is active against most bacteria, filamentous fungi, yeast, plant and animal cells.
Use of phleomycin is recommended for cells poorly sensitive to Zeocin™, i.e. filamentous fungi and some yeasts. Phleomycin resistance is conferred by the Sh ble gene from Streptoalloteichus hindustanus which encodes a protein that binds to phleomycin, inhibiting its DNA cleavage activity.

Typically, phleomycin is used at a concentration of 10 µg/ml for yeasts and 25-150 µg/ml for filamentous fungi.

Specifications

Product concentration: 20 mg/ml in solution
CAS number: 11006-33-0

Quality Control: Each lot is thoroughly tested to ensure the absence of lot-to-lot variation.
Endotoxin level: < 1 EU/mg
Physicochemical characterization: HPLC, pH, appearance
Cell culture tested: potency validated in phleomycin-sensitive and phleomycin-resistant bacterial, fungal and mammalian cells
Non-cytotoxicity of trace contaminants: absence of long-term effects confirmed in phleomycin-resistant cells

Contents

Phleomycin is supplied as a sterile filtered blue solution at 20 mg/ml in HEPES buffer. This product is available in three pack sizes:
- ant-ph-1: 5 x 1 ml (100 mg)
- ant-ph-5: 25 x 1 ml (500 mg)
- ant-ph-5b: 1 x 25 ml (500 mg)

Phleomycin is also supplied as a blue powder:
- ant-ph-2p: 250 mg
- ant-zn-5p: 2 x 250 mg (500 mg)
- ant-zn-10p: 1 g

Phleomycin is shipped at room temperature. Upon receipt it should be stored at 4°C or -20°C.

ORDERING

Phleomycin (solution)

Description Selective antibiotic for the Sh ble gene
Cat. Codeant-ph-1
Unit Size100 mg (5 x 1 ml)
Price For price or distributor address,
please select your country

Phleomycin (solution)

Description Selective antibiotic for the Sh ble gene
Cat. Codeant-ph-5
Unit Size500 mg (25 x 1 ml)
Price For price or distributor address,
please select your country

Phleomycin (solution)

Description Selective antibiotic for the Sh ble gene
Cat. Codeant-ph-5b
Unit Size500 mg (25 ml)
Price For price or distributor address,
please select your country

Phleomycin (powder)

Description Selective antibiotic for the Sh ble gene
Cat. Codeant-ph-2p
Unit Size250 mg
Price For price or distributor address,
please select your country

Phleomycin (powder)

Description Selective antibiotic for the Sh ble gene
Cat. Codeant-ph-5p
Unit Size500 mg
Price For price or distributor address,
please select your country

Phleomycin (powder)

Description Selective antibiotic for the Sh ble gene
Cat. Codeant-ph-10p
Unit Size1 g
Price For price or distributor address,
please select your country
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