Blasticidin

Selective anibiotic blasticidinBlasticidin is an efficient selective antibiotic that acts on both eukaryotic and prokaryotic cells.  Blasticidin is a peptidyl nucleoside antibiotic isolated from Streptomyces griseochromogenes that inhibits protein synthesis by interfering with the peptide-bond formation in the ribosomal machinery.

Resistance to blasticidin is conferred by the blasticidin resistance gene from Bacillus cereus (bsr), which codes for blasticidin-S deaminase.

Cell death induced by blasticidin is rapid and cell lines carrying a blasticidin resistance gene can be obtained in less than a week. Typically, mammalian cells are sensitive to blasticidin concentrations of 1-10 µg/ml, and bacteria to 25-100 µg/ml.

Specifications

Product concentration: 10 mg/ml
Working concentration:
- 25-100 µg/ml in bacteria
- 1-30 µg/ml in mammalian cells
Purity: controlled by HPLC >95%
Formula: C17H26N8O5, 1 HCl
Quality control:
Blasticidin hydrochloride is supplied as colorless solution in HEPES buffer (100% active compound), pH 7.5, filtered to sterility for customer convenience and cell culture tested, or as a powder.
Activity controlled by bioassays on bacteria and mammalian cell lines.

Contents

Blasticidin hydrochloride is supplied as 1 ml tubes of a 10 mg/ml colorless solution in HEPES buffer (100% active compound):
- ant-bl-1: 10 x 1 ml at 10 mg/ml (100 mg)
- ant-bl-5: 50 x 1 ml at 10 mg/ml (500 mg)
- ant-bl-5b: 1 x 50ml at 10 mg/ml (500 mg)

Blasticidin hydrochloride is also supplied as a powder:
-  ant-bl-10p: 1 g (powder)

Blasticidin is shipped at room temperature. Upon receipt it should be stored at 4°C or -20°C.

Ref.

- PEREZ-GONZALEZ J.A. et al. (1990). Gene.86: 129-134.
IZUMIM. et al. (1991). Exp.Cell Res.197: 229-233.
ITAYA M. et al. (1990). J.Biochem. 107: 799-801.
- KIMURA M. et al. (1994). Mol. Gen. Genet. 242: 121-129.
- KIMURA M. et al. (1994). Biochim.Biophys.Acta.1219:653-659

Citations

Recent articles using Blasticidin

ORDERING

Blasticidin (solution)

Cat. Code ant-bl-1
Unit Size 100 mg (10 x 1 ml)
Price For price or distributor address,
please select your country

Blasticidin (solution)

Cat. Code ant-bl-5b
Unit Size 500 mg (50 ml bottle)
Price For price or distributor address,
please select your country

Blasticidin (solution)

Cat. Code ant-bl-5
Unit Size 500 mg (50 x 1 ml)
Price For price or distributor address,
please select your country

Blasticidin (powder)

Cat. Code ant-bl-10p
Unit Size 1 g
Price For price or distributor address,
please select your country
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