Blasticidin

Selection antibiotic: cell culture tested, sterile reagent

Selective anibiotic blasticidinBlasticidin is an efficient selective antibiotic that acts on both eukaryotic and prokaryotic cells.  Blasticidin is a peptidyl nucleoside antibiotic isolated from Streptomyces griseochromogenes that inhibits protein synthesis by interfering with the peptide-bond formation in the ribosomal machinery.

Resistance to blasticidin is conferred by the blasticidin resistance gene from Bacillus cereus (bsr), which codes for blasticidin-S deaminase.

Cell death induced by blasticidin is rapid and cell lines carrying a blasticidin resistance gene can be obtained in less than a week. Typically, mammalian cells are sensitive to blasticidin concentrations of 1-10 µg/ml, and bacteria to 25-100 µg/ml.

Specifications

Product concentration: 10 mg/ml

Quality Control:
Each lot is thoroughly tested to ensure the absence of lot-to-lot variation.
Purity:
≥ 95% (HPLC)
Endotoxin level:
< 1 EU/mg
Physicochemical characterization:
pH, appearance
Cell-culture tested:
potency validated in blasticidin-sensitive and blasticidin-resistant mammalian cell lines
Non-cytotoxicity of trace contaminants:
absence of long-term effects confirmed in blasticidin-resistant cells

Contents

Blasticidin hydrochloride is supplied as a sterile filtered solution at 10 mg/ml in HEPES buffer. This product is available in three pack sizes:
- ant-bl-1: 10 x 1 ml (100 mg)
- ant-bl-5: 50 x 1 ml (500 mg)
- ant-bl-5b: 1 x 50ml (500 mg)

Blasticidin hydrochloride is also supplied as a powder:
-  ant-bl-10p: 1 g (powder)

Blasticidin is shipped at room temperature. Upon receipt it should be stored at 4°C or -20°C.

Citations

Recent articles using InvivoGen Blasticidin

ORDERING

Blasticidin (solution)

Cat. Code ant-bl-1
Unit Size 100 mg (10 x 1 ml)
Price For price or distributor address,
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Blasticidin (solution)

Cat. Code ant-bl-5b
Unit Size 500 mg (50 ml bottle)
Price For price or distributor address,
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Blasticidin (solution)

Cat. Code ant-bl-5
Unit Size 500 mg (50 x 1 ml)
Price For price or distributor address,
please select your country

Blasticidin (powder)

Cat. Code ant-bl-10p
Unit Size 1 g
Price For price or distributor address,
please select your country
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Note that the sequence of available ORFs provided by InvivoGen can differ from a given reference Genbank record due to genetic variations and/or alternative splicing. Customers should verify that the version of a gene sold by InvivoGen is suitable for the customer needs.
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