Blasticidin
InvivoGen produces Blasticidin, an efficient selective antibiotic that acts on both eukaryotic and prokaryotic cells. Blasticidin is a peptidyl nucleoside antibiotic isolated from Streptomyces griseochromogenes that inhibits protein synthesis by interfering with the peptide-bond formation in the ribosomal machinery.
Resistance to blasticidin is conferred by the blasticidin resistance gene from Bacillus cereus (bsr), which codes for blasticidin-S deaminase.
Cell death induced by blasticidin is rapid and cell lines carrying a blasticidin resistance gene can be obtained in less than a week. Typically, mammalian cells are sensitive to blasticidin concentrations of 1-10 µg/ml, and bacteria to 25-100 µg/ml.
Specifications
Working concentration:
- 25-100 µg/ml in bacteria
- 1-10 µg/ml in mammalian cells
CAS number: 3513-03-9
Purity: controlled by HPLC >95%
Molecular weight: 458.9
Formula: C17H26N8O5, 1 HCl
Quality control:
Blasticidin hydrochloride is supplied as colorless solution in HEPES buffer (100% active compound), pH 7.5, filtered to sterility for customer convenience and cell culture tested, or as a powder.
Activity controlled by bioassays on bacteria and mammalian cell lines.
Docs
- TDS Blasticidin (solution) : 500 mg (50 x 1 ml) (ant-bl-5) , 100 mg (10 x 1 ml) (ant-bl-1) , 500 mg (50 ml bottle) (ant-bl-5b)
- TDS Blasticidin (powder) : 1 g (ant-bl-10p)
- MSDS Blasticidin (solution) : 500 mg (50 x 1 ml) (ant-bl-5) , 100 mg (10 x 1 ml) (ant-bl-1) , 500 mg (50 ml bottle) (ant-bl-5b)
- MSDS Blasticidin (powder) : 1 g (ant-bl-10p)
Contents
Blasticidin hydrochloride is supplied as 1 ml tubes of a 10 mg/ml colorless solution in HEPES buffer (100% active compound):
- ant-bl-1: 10 x 1 ml at 10 mg/ml (100 mg)
- ant-bl-5: 50 x 1 ml at 10 mg/ml (500 mg)
- ant-bl-5b: 1 x 50ml at 10 mg/ml (500 mg)
Blasticidin hydrochloride is also supplied as a powder:
- ant-bl-10p: 1 g (powder)
Blasticidin is shipped at room temperature. Upon receipt it should be stored at 4°C or -20°C.
Details
Ref.
- PEREZ-GONZALEZ J.A. et al. (1990). Gene.86: 129-134.
- IZUMIM. et al. (1991). Exp.Cell Res.197: 229-233.
- ITAYA M. et al. (1990). J.Biochem. 107: 799-801.
- KIMURA M. et al. (1994). Mol. Gen. Genet. 242: 121-129.
- KIMURA M. et al. (1994). Biochim.Biophys.Acta.1219:653-659
Citations
Recent articles using Blasticidin
- 2011 - J.Virol., 85: 4792-4801
A respiratory syncytial virus replicon that is noncytotoxic and capable of long-term foreign gene expression.
Malykhina O, Yednak MA, Collins PL, Olivo PD, Peeples ME
Free article - 2012 - J. Biol. Chem., 287: 1306 - 1321
Plasmodium falciparum inhibitor-3 homolog increases protein phosphatase type 1 activity and is essential for parasitic survival.
Freville A, Landrieu I, Garcia-Gimeno MA, Vicogne J, Montbarbon M, Bertin B, Verger A, Kalamou H, Sanz P, Werkmeister E, Pierrot C, Khalife J - 2011 - Nucleic Acids Res., 39: 7750 - 7763
PDE12 removes mitochondrial RNA poly(A) tails and controls translation in human mitochondria.
Rorbach J, Nicholls TJ, Minczuk M
Free article
ORDERING
Blasticidin (solution)
| Cat. Code | ant-bl-1 |
|---|---|
| Unit Size | 100 mg (10 x 1 ml) |
| Price | For price or distributor address, please select your country |
Blasticidin (solution)
| Cat. Code | ant-bl-5b |
|---|---|
| Unit Size | 500 mg (50 ml bottle) |
| Price | For price or distributor address, please select your country |
Blasticidin (solution)
| Cat. Code | ant-bl-5 |
|---|---|
| Unit Size | 500 mg (50 x 1 ml) |
| Price | For price or distributor address, please select your country |
Blasticidin (powder)
| Cat. Code | ant-bl-10p |
|---|---|
| Unit Size | 1 g |
| Price | For price or distributor address, please select your country |