Puromycin
Puromycin is an aminonucleoside antibiotic produced by Streptomyces alboniger. It specifically inhibits peptidyl transfer on both prokaryotic and eukaryotic ribosomes. This antibiotic inhibits the growth of Gram positive bacteria and various animal and insect cells. Puromycin can also be used in some particular conditions for the selection of E. coli transformants.
Resistance to puromycin is conferred by the Pac gene encoding a puromycin N-acetyl-transferase (PAC) that was found in a Streptomyces producer strain.
Animal cells are generally sensitive to concentrations from 1 to 10 µg/ml.
Specifications
Working concentration:1 to 10 µg/ml in animal cells
CAS number: 58-58-2
Purity: >95%
Molecular weight: 471.51
Formula: C22H29N7O5, 2HCl
Documents
Contents
Puromycin hydrochloride is supplied as 2ml tubes of a 10mg/ml solution in HEPES buffer in different formats:
- 5 x 2ml at 10 mg/ml (100 mg)
- 25 x 2ml at 10 mg/ml (500 mg)
Details
Ref.
- VARA J., et al. (1985).Biosynthesis of puromycin by Streptomyces alboniger: characterization of puromycin N-acetyltransferase. Biochemistry. 24: 8074-8081
- LACALLE R.A., et al. (1989). Molecular analysis of the pac gene encoding a puromycin N-acetyl transferase from Streptomyces alboniger. Gene .79: 375-380
- DE LA LUNA S and J.ORTIN. (1992). Methods In Enzymology.216:376-385
ORDERING
Puromycin
| Cat. Code | ant-pr-1 |
|---|---|
| Unit Size | 100 mg (5 x 2 ml) |
| Price | For price or distributor address, please select your country |
Puromycin
| Cat. Code | ant-pr-5 |
|---|---|
| Unit Size | 500 mg (25 x 2 ml) |
| Price | For price or distributor address, please select your country |
