Puromycin

Puromycin is an aminonucleoside antibiotic produced by Streptomyces alboniger. It specifically inhibits peptidyl transfer on both prokaryotic and eukaryotic ribosomes. This antibiotic inhibits the growth of Gram positive bacteria and various animal and insect cells. Puromycin can also be used in some particular conditions for the selection of E. coli transformants.
Resistance to puromycin is conferred by the Pac gene encoding a puromycin N-acetyl-transferase (PAC) that was found in a Streptomyces producer strain.

Animal cells are generally sensitive to concentrations from 1 to 10 µg/ml.

Specifications

Product concentration: 10 mg/ml
Working concentration:
1 to 10 µg/ml in animal cells
CAS number
: 58-58-2
Purity:
>95%
Molecular weight:
471.51
Formula:
C22H29N7O5, 2HCl

Contents

Puromycin hydrochloride is supplied as 1ml tubes of a 10 mg/ml solution in HEPES buffer (100% active product), filtered to sterility for customer convenience, and cell culture tested.
- ant-pr-1: 10 x 1 ml at 10 mg/ml (100 mg)
- ant-pr-5: 50 x 1 ml at 10 mg/ml (500 mg)

Puromycin is shipped at room temperature. Upon receipt it should be stored at 4°C or -20°C.

Details

3D animation of a puromycin molecule

Ref.

- VARA J., et al. (1985).Biosynthesis of puromycin by Streptomyces alboniger: characterization of puromycin N-acetyltransferase. Biochemistry. 24: 8074-8081
- LACALLE R.A., et al. (1989).
Molecular analysis of the pac gene encoding a puromycin N-acetyl transferase from Streptomyces alboniger. Gene .79: 375-380
- DE LA LUNA S and J.ORTIN. (1992). Methods In Enzymology.216:376-385

Citations

Recent articles using Puromycin

ORDERING

Puromycin

Cat. Code ant-pr-1
Unit Size 100 mg (10 x 1 ml)
Price For price or distributor address,
please select your country

Puromycin

Cat. Code ant-pr-5
Unit Size 500 mg (50 x 1 ml)
Price For price or distributor address,
please select your country

Puromycin

Cat. Code ant-pr-5b
Unit Size 500 mg (1 x 50 ml)
Price For price or distributor address,
please select your country
Disclaimer: Our products are provided for research purpose only. Commercial applications may require licensing from third parties.
Note that the sequence of available ORFs provided by InvivoGen can differ from a given reference Genbank record due to genetic variations and/or alternative splicing. Customers should verify that the version of a gene sold by InvivoGen is suitable for the customer needs.
Copyrights © 2011-2013 InvivoGen. All Rights Reserved. Reproduction of any materials from this site is strictly forbidden without permission for commercial use. Nonprofit use for non-commercial research and educational purposes is permitted, citation should include the URL "www.invivogen.com".