Puromycin

Selection antibiotic: cell culture tested, sterile reagent

Puromycin is an aminonucleoside antibiotic produced by Streptomyces alboniger. It specifically inhibits peptidyl transfer on both prokaryotic and eukaryotic ribosomes. This antibiotic inhibits the growth of Gram positive bacteria and various animal and insect cells. Puromycin can also be used in some particular conditions for the selection of E. coli transformants.
Resistance to puromycin is conferred by the Pac gene encoding a puromycin N-acetyl-transferase (PAC) that was found in a Streptomyces producer strain.

Animal cells are generally sensitive to concentrations from 1 to 10 µg/ml.

Specifications

Product concentration: 10 mg/ml
CAS number: 58-58-2

Quality Control: Each lot is thoroughly tested to ensure the absence of lot-to-lot variation.
Purity:
≥ 98% (HPLC)
Endotoxin level:
< 5 EU/mg
Physicochemical characterization
Cell-culture tested:
potency validated in puromycin-sensitive and puromycin‑resistant mammalian cell lines
Non-cytotoxicity of trace contaminants:
absence of long-term effects confirmed in puromycin-resistant cells

Contents

Puromycin dihydrochloride is supplied as a sterile filtered solution at 10 mg/ml in HEPES buffer. This product is available in three pack sizes:
- ant-pr-1: 10 x 1 ml (100 mg)
- ant-pr-5: 50 x 1 ml (500 mg)
- ant-pr-5b: 1 x 50 ml (500 mg)

Puromycin is shipped at room temperature. Upon receipt it should be stored at 4°C or -20°C.

Details

 

Citations

Recent articles using InvivoGen Puromycin

ORDERING

Puromycin

Cat. Code ant-pr-1
Unit Size 100 mg (10 x 1 ml)
Price For price or distributor address,
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Puromycin

Cat. Code ant-pr-5
Unit Size 500 mg (50 x 1 ml)
Price For price or distributor address,
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Puromycin

Cat. Code ant-pr-5b
Unit Size 500 mg (1 x 50 ml)
Price For price or distributor address,
please select your country
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