Selection antibiotic: cell culture tested, sterile reagent

Puromycin is an aminonucleoside antibiotic produced by Streptomyces alboniger. It specifically inhibits peptidyl transfer on both prokaryotic and eukaryotic ribosomes. This antibiotic inhibits the growth of Gram positive bacteria and various animal and insect cells. Puromycin can also be used in some particular conditions for the selection of E. coli transformants.
Resistance to puromycin is conferred by the Pac gene encoding a puromycin N-acetyl-transferase (PAC) that was found in a Streptomyces producer strain.

Animal cells are generally sensitive to concentrations from 1 to 10 µg/ml.


Product concentration: 10 mg/ml
Working concentration:
1 to 10 µg/ml in animal cells
CAS number
: 58-58-2
Molecular weight:
C22H29N7O5, 2HCl


Puromycin hydrochloride is supplied as 1ml tubes of a 10 mg/ml solution in HEPES buffer (100% active product), filtered to sterility for customer convenience, and cell culture tested.
- ant-pr-1: 10 x 1 ml at 10 mg/ml (100 mg)
- ant-pr-5: 50 x 1 ml at 10 mg/ml (500 mg)

Puromycin is shipped at room temperature. Upon receipt it should be stored at 4°C or -20°C.


3D animation of a puromycin molecule


- VARA J., et al. (1985).Biosynthesis of puromycin by Streptomyces alboniger: characterization of puromycin N-acetyltransferase. Biochemistry. 24: 8074-8081
- LACALLE R.A., et al. (1989).
Molecular analysis of the pac gene encoding a puromycin N-acetyl transferase from Streptomyces alboniger. Gene .79: 375-380
- DE LA LUNA S and J.ORTIN. (1992). Methods In Enzymology.216:376-385


Recent articles using InvivoGen Puromycin



Cat. Code ant-pr-1
Unit Size 100 mg (10 x 1 ml)
Price For price or distributor address,
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Cat. Code ant-pr-5
Unit Size 500 mg (50 x 1 ml)
Price For price or distributor address,
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Cat. Code ant-pr-5b
Unit Size 500 mg (1 x 50 ml)
Price For price or distributor address,
please select your country
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