QUANTI-Blue™

QUANTI-Blue™ is a colorimetric enzyme assay developed to determine any alkaline phosphatase (AP) activity in a biological sample, such as supernatants of cell cultures.

InvivoGen provides a wide collection of reporter cell lines with the secreted embryonic alkaline phosphatase (SEAP) reporter gene encoding a truncated form of placental AP.

SEAP is secreted into cell culture supernatant and therefore shows many advantages over intracellular reporters.

More convenient than the powder format, the liquid formulation of QUANTI-Blue™ offers an easy and rapid means to detect and quantify SEAP and AP activity.

In the presence of AP, the color of QUANTI-Blue™ changes from pink to purple/blue.

The purple/blue color intensity reflects the activity of AP. The levels of AP can be determined qualitatively with the naked eye or quantitatively using a spectrophotometer at 620-655 nm.

Description

QUANTI-Blue™ Solution:

  • Short hands-on-time: < 10 min.
  • Easy to prepare: just mix QB Reagent and QB Buffer with water.
  • Highly sensitive.
  • High saturation threshold.
  • Allows high-throughput screening.

QUANTI-Blue™ Powder :

  • Short hands-on-time: < 10 min.
  • Easy to prepare: just add water.
  • Highly sensitive.
  • High saturation threshold.
  • Allows high-throughput screening.

Contents

QUANTI-Blue™ (Solution) is available in two pack sizes:
• rep-qbs : 5 x 1 ml of QB reagent + 5 x 1 ml QB buffer, sufficient to prepare 500 ml of QUANTI-Blue™
• rep-qbs2 : 10 x 1 ml of QB reagent + 10 x 1 ml QB buffer, sufficient to prepare 1 liter of QUANTI-Blue™

QUANTI-Blue™ (Powder) is available in two pack sizes:
• rep-qb1 : 5-pouch unit, sufficient to prepare 5 x 100 ml of QUANTI-Blue™ 
• rep-qb2 : 10-pouch unit, sufficient to prepare 10 x 100 ml of QUANTI-Blue™

Details

FEATURES AND BENEFITS

  • Requires small samples of cell supernatants - Samples of 20 μl are sufficient.
  • No need to process samples - Preparation of cell lysates or heating of samples are not required.
  • Determine secreted AP activity without disturbing cells - The same cell cultures can be repeatedly sampled for kinetic studies or further experimentation.
  • Assay can be completed in 30 min - Hands-on time no longer than 10 min. The enzymatic activity can be detected as early as 30 min after incubation of the samples in QUANTI-Blue™.
  • Wide dynamic range allows to detect low and high levels of AP - No need to perform multiple sample dilutions.
  • Highly sensitive for quantitative measurement - Higher saturation threshold than with pNPP (p-nitrophenyl phosphate) resulting in more significant differences between non or low AP expression and high AP expression.
  • Extremely simple to use - 1) prepare solution with water, 2) add sample, incubate at 37 °C and 3) assess AP activity with the naked eye or by reading the optical density (OD) at 625-655 nm.

Citations

Recent articles using InvivoGen QUANTI-Blue™

ORDERING

QUANTI-Blue™ (solution)NEW!

Description Medium for detection and quantification of alkaline phosphatase
Cat. Coderep-qbs2
Unit Size10 ml
Price For price or distributor address,
please select your country

QUANTI-Blue™ (solution)NEW!

Description Medium for detection and quantification of alkaline phosphatase
Cat. Coderep-qbs
Unit Size5 ml
Price For price or distributor address,
please select your country

QUANTI-Blue™ (powder)

Description Alkaline phosphatase detection medium
Cat. Coderep-qb1
Unit Size5 pouches
Price For price or distributor address,
please select your country

QUANTI-Blue™ (powder)

Description Alkaline phosphatase detection medium
Cat. Coderep-qb2
Unit Size10 pouches
Price For price or distributor address,
please select your country
Disclaimer: Our products are provided for research purpose only. Commercial applications may require licensing from third parties.
Note that the sequence of available ORFs provided by InvivoGen can differ from a given reference Genbank record due to genetic variations and/or alternative splicing. Customers should verify that the version of a gene sold by InvivoGen is suitable for the customer needs.
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