QUANTI-Blue™

QUANTI-Blue™ is a colorimetric enzyme assay developed to determine any alkaline phosphatase activity (AP) in a biological sample, such as supernatants of cell cultures. In particular, QUANTI-Blue™ provides an easy and rapid mean to detect and quantify secreted embryonic alkaline phosphatase (SEAP), a reporter widely used for in vitro and in vivo analytical studies.

In the presence of alkaline phosphatase, the color of QUANTI-Blue™ changes from pink to purple/blue. The intensity of the blue hue reflects the activity of AP. The levels of AP can be determined qualitatively with the naked eye or quantitatively using a spectrophotometer at 620-655 nm.

QUANTI-Blue™ is useful to monitor activation in reporter cell lines.

Specifications

Short hands-on-time: < 10 min

Easy to prepare: just add water

Highly sensitive

High saturation threshold

Allows high-throughput screening

Contents

5-pouch unit or 10-pouch unit

Each pouch allows the preparation of 100 ml of QUANTI-Blue™.

ORDERING

QUANTI-Blue™

Description Alkaline phosphatase detection medium
Cat. Code rep-qb1
Unit Size 5 pouches (5 x 100 ml)
Price For price or distributor address,
please select your country

QUANTI-Blue™

Description Alkaline phosphatase detection medium
Cat. Code rep-qb2
Unit Size 10 pouches (10 x 100 ml)
Price For price or distributor address,
please select your country
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Note that the sequence of available ORFs provided by InvivoGen can differ from a given reference Genbank record due to genetic variations and/or alternative splicing. Customers should verify that the version of a gene sold by InvivoGen is suitable for the customer needs.
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