HEK-Blue™ Detection
HEK-Blue™ Detection is a cell culture medium that allows the detection of SEAP as the reporter protein is secreted by the cells.
HEK-Blue™ Detection contains all the nutrients necessary for cell growth and a specific SEAP color substrate. The hydrolysis of the substrate by SEAP produces a purple/blue color that can be easily detected with the naked eye or measured with a spectrophotometer.
Specifications
One step procedure
No need to process samples
Extremely simple to use
Designed for high-throughput screening
Documents
Contents
HEK-Blue™ Detection is provided in sealed pouches and is available in two quantities:
- 5 pouches
- 10 pouches
Each pouch allows the preparation of 50 ml of HEK-Blue™ Detection.
Description
HEK-Blue™ Detection is a cell culture medium developed to provide a fast and convenient method to monitor SEAP expression. Detection of SEAP occurs as the reporter protein is secreted by the cells grown in HEK-Blue™ Detection. HEK-Blue™ Detection changes to a purple/blue color in the presence of alkaline phosphatase activity. SEAP is a widely used reporter gene. It is a truncated form of placental alkaline phosphatase, a GPI-anchored protein.
SEAP is secreted into cell culture supernatant and therefore offers many advantages over intracellular reporters. It allows to determine reporter activity without disturbing the cells, does not require the preparation of cell lysates and can be used for kinetic studies. Using HEK-Blue™ Detection, SEAP expression can be observed visually, and unlike flourescent or luminescent reporters can be easily quantified using a microplate reader or spectrophotometer.
HEK-Blue™ Detection is applicable to high-throughput screening.
ORDERING
HEK-Blue™ Detection
| Cat. Code | hb-det2 |
|---|---|
| Unit Size | 5 pouches |
| Price | For price or distributor address, please select your country |
HEK-Blue™ Detection
| Cat. Code | hb-det3 |
|---|---|
| Unit Size | 10 pouches |
| Price | For price or distributor address, please select your country |
