HEK-Blue™ Detection

HEK-Blue™ Detection is a cell culture medium that allows the detection of SEAP as the reporter protein is secreted by the cells.

HEK-Blue™ Detection contains all the nutrients necessary for cell growth and a specific SEAP color substrate. The hydrolysis of the substrate by SEAP produces a purple/blue color that can be easily detected with the naked eye or measured with a spectrophotometer.


Description: Cell culture medium

• Real-time detection of SEAP produced by cells
• Applicable to high-throughput screening

Readout Method:
• Naked eye (purple/blue color)
• Spectrophotometry (620 - 655 nm)


HEK-Blue™ Detection is provided in sealed pouches and is available in two quantities:

-  5 pouches

-  10 pouches

Each pouch allows the preparation of 50 ml of HEK-Blue™ Detection.

Store at 2-8°C.
Pouches are stable 6 months at 2-8°C.
After preparation, product is stable 2 weeks at 2-8°C and 2 months at -20°C.


HEK-Blue™ Detection is a cell culture medium developed to provide a fast and convenient method to monitor SEAP expression. Detection of SEAP occurs as the reporter protein is secreted by the cells grown in HEK-Blue™ Detection. HEK-Blue™ Detection changes to a purple/blue color in the presence of alkaline phosphatase activity. SEAP is a widely used reporter gene. It is a truncated form of placental alkaline phosphatase, a GPI-anchored protein.

SEAP is secreted into cell culture supernatant and therefore offers many advantages over intracellular reporters. It allows to determine reporter activity without disturbing the cells, does not require the preparation of cell lysates and can be used for kinetic studies. Using HEK-Blue™ Detection, SEAP expression can be observed visually, and unlike fluorescent or luminescent reporters can be easily quantified using a microplate reader or spectrophotometer.

HEK-Blue™ Detection is applicable to high-throughput screening.


1. Resuspend HEK-Blue™ Detection
2.Add SEAP-expressing cells
3.Transfer to multi-well plate
4. Incubate 6 to 24 hours at 37°C, 5% CO2
5. Assess SEAP levels


Recent articles using InvivoGen HEK-Blue™ Detection


HEK-Blue™ Detection

Cat. Codehb-det2
Unit Size5 pouches
Price For price or distributor address,
please select your country

HEK-Blue™ Detection

Cat. Codehb-det3
Unit Size10 pouches
Price For price or distributor address,
please select your country
Disclaimer: Our products are provided for research purpose only. Commercial applications may require licensing from third parties.
Note that the sequence of available ORFs provided by InvivoGen can differ from a given reference Genbank record due to genetic variations and/or alternative splicing. Customers should verify that the version of a gene sold by InvivoGen is suitable for the customer needs.
Copyrights © 2011-2016 InvivoGen. All Rights Reserved. Reproduction of any materials from this site is strictly forbidden without permission for commercial use. Nonprofit use for non-commercial research and educational purposes is permitted, citation should include the URL "www.invivogen.com".