RLR Reporter Cell Lines

RIG-I-like receptors (RLRs) constitute a family of three cytoplasmic RNA helicases that are critical for host antiviral responses. RIG-I (retinoic-acid-inducible protein 1) and MDA-5 (melanoma-differentiation-associated gene 5) sense double-stranded RNA (dsRNA), a replication intermediate for RNA viruses. A third RLR has been described: LGP2. LGP2 contains a RNA binding domain but lacks the CARD domains and thus acts as a negative feedback regulator of RIG-I and MDA-5.

InvivoGen offers several cell lines to study the RLR pathway: A549-Dual™ cells, which derive from the human A549 lung carcinoma cell line; B16-Blue™ IFN-α/β, which derive from murine B16 melanoma cells;  HEK-Lucia™ RIG-I, an engineered human embryonic kidney (HEK) 293 cell line; RAW-Blue™ ISG cells, a mouse macrophage reporter cell line; and THP1-Blue™ ISG, which derive from human THP-1 monocytes. These cells express an inducible reporter (SEAP or Lucia luciferase) system to facilitate the monitoring of RLR-induced IRF3/7 and/or NF-κB activation.

A549

A549-Dual™ CellsHuman NF-κB-SEAP & IRF-Luc Reporter lung carcinoma3-7 x 10e6 cells
A549-Dual™ KO-RIG-I CellsRIG-I knockout NF-kB-SEAP & IRF-Lucia Reporter Cell Line3-7 x 10e6 cells
A549-Dual™ KO-MDA5 cellsMDA-5 knockout NF-kB-SEAP & IRF-Lucia Reporter Cell Line3-7 x 10e6 cells
A549-Dual™ KO-MAVS cells NEWMAVS knockout NF-kB-SEAP & IRF-Lucia Reporter Cell Line3-7 x 10e6 cells

B16

B16-Blue™ IFN-α/β CellsMurine B16 melanoma - Type I IFNs reporter cells3-7 x 10e6 cells

HEK293

HEK-Lucia™ RIG-I Cells NEWLucia luciferase reporter HEK293 cells expressing human RIG-I3-7 x 10e6 cells
HEK-Lucia™ Null Cells NEWLucia luciferase reporter HEK293 cells3-7 x 10e6 cells

RAW-Blue

RAW-Blue™ ISG CellsIRF-SEAP-Reporter Cell Line3-7 x 10e6 cells

RAW-Lucia

RAW-Lucia™ ISG CellsIRF-Lucia Luciferase Reporter Cell Line3-7 x 10e6 cells
RAW-Lucia™ ISG-KO-RIG-I CellsRIG-I Knockout IRF-Lucia Luciferase Reporter Cell Line3-7 x 10e6 cells
RAW-Lucia™ ISG-KO-MDA5 CellsMDA-5 Knockout IRF-Lucia Luciferase Reporter Cell Line3-7 x 10e6 cells
RAW-Lucia™ ISG-KO-MAVS Cells NEWMAVS-Knockout IRF-Lucia Luciferase Reporter Cell Line3-7 x 10e6 cells

THP1

THP1-Blue™ ISG CellsHuman THP-1 Monocytes - IRF SEAP Reporter3-7 x 10e6 cells
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Note that the sequence of available ORFs provided by InvivoGen can differ from a given reference Genbank record due to genetic variations and/or alternative splicing. Customers should verify that the version of a gene sold by InvivoGen is suitable for the customer needs.
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