HEK-Dual™ hTLR9 (NF/IL8) cells were generated from HEK-Dual™ Null cells by stable transfection of the human TLR9 (hTLR9) gene. Due to the knockout of TLR3 and TLR5, these cells enable the study of hTLR9 signaling without interference from other TLRs. They respond to very low concentrations of TLR9 agonists, such as the class B CpG oligodeoxynucleotide (CpG ODN) ODN 2006. They do not respond to other TLR agonists or to the cytokine TNF-α (see validation sheet).

HEK-Dual™ hTLR9 (NF/IL8) cells are resistant to hygromycin B, Zeocin™ and blasticidin. They should be maintained in growth medium supplemented with hygromycin B and Zeocin™.

Figures for this product

NF-kB (SEAP) responseKI-IL-8 (Lucia) response


Antibiotic resistance: blasticidinhygromycinZeocin™

Growth medium: DMEM, 4.5 g/l glucose, 2 mM L-glutamine, 10% (v/v) fetal bovine serum, 50 U/ml penicillin, 50 μg/ml streptomycin, 100 μg/ml Normocin™

Guaranteed mycoplasma-free

Shipped on dry ice


- 1 vial containing 3-7 x 106 cells.
- 100 μl Hygromycin B Gold (100 mg/ml).
- 100 μl Zeocin™ (100mg/ml).
- 1 ml Normocin™ (50 mg/ml).
- 1 pouch of QUANTI-Blue™ (SEAP detection medium).
- 1 pouch of QUANTI-Luc™ (Lucia luciferase detection medium).


  1. Ohta K. et al., 2014. TLR-mediated interleukin-8 production by human submandibular gland epithelial cells. Mol Med Rep. 10(5):2377-82.
  2. Roebuck KA. et al., 1999. Regulation of interleukin-8 gene expression. J Interferon Cytokine Res. 19(5):429-38. 


HEK-Dual™ hTLR9 (NF/IL8)NEW!

Description Double readout TLR cells
Cat. Codehkd-htlr9ni
Unit Size3-7 x 10e6 cells
Price For price or distributor address,
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