KO Cell Lines

InvivoGen provides a series of knockout (KO) cell lines generated by gene editing in popular cell lines, such as RAW 264.7 mouse macrophage and THP-1 human monocyte.

The genes targeted for KO are involved in the interferon (IFN) response to nucleic acids (e.g. double-stranded DNA or RNA) and to cyclic dinucleotides (CDNs). These genes include those encoding for the cytosolic DNA sensor cGAS, the cytosolic RNA sensor RIG-I, the cytosolic CDN sensor (and cGAS adaptor) STING, and the IFN signaling mediator TBK1.

Coupled with their parental cell lines, InvivoGen’s KO cell lines are utile for studying the function of these genes and for screening for molecules that activate pathways involving the proteins encoded by these genes.

The KO status of each cell line is thoroughly tested as follows:
  • Biallelic mutation/deletion confirmed by PCR and DNA sequencing;
  • Reporter activity validated by functional assays; and
  • KO stability guaranteed up to 20 passages.

KO Cell Lines

A549-Dual™ KO-RIG-I CellsNEWRIG-I knockout NF-kB-SEAP & IRF-Lucia Reporter Cell Line

HEK-293 embryonic kidney cells

HEK-Blue™ ISG-KO-STING CellsNEWSTING Knockout IRF Reporter Cell Line

B16 melanoma

B16-Blue™ ISG-KO-STING CellsMurine B16 melanoma - STING Knockout IRF-reporter cells

RAW 264.7 macrophages

RAW-Lucia™ ISG-KO-cGAS CellscGAS Knockout IRF-Lucia Luciferase Reporter Cell Line
RAW-Lucia™ ISG-KO-IFI16 CellsIFI16 Knockout IRF-Lucia Luciferase Reporter Cell Line
RAW-Lucia™ ISG-KO-IRF3 CellsIRF3 Knockout IRF-Lucia Luciferase Reporter Cell Line
RAW-Lucia™ ISG-KO-IRF7 CellsIRF7 Knockout IRF-Lucia Luciferase Reporter Cell Line
RAW-Lucia™ ISG-KO-MAVS CellsNEWMAVS-Knockout IRF-Lucia Luciferase Reporter Cell Line
RAW-Lucia™ ISG-KO-MDA5 CellsMDA-5 Knockout IRF-Lucia Luciferase Reporter Cell Line
RAW-Lucia™ ISG-KO-RIG-I CellsRIG-I Knockout IRF-Lucia Luciferase Reporter Cell Line
RAW-Lucia™ ISG-KO-STING CellsSTING Knockout IRF-Lucia Luciferase Reporter Cell Line
RAW-Lucia™ ISG-KO-TBK1 CellsTBK1 Knockout IRF-Lucia Luciferase Reporter Cell Line
RAW-Lucia™ ISG-KO-TREX1 CellsTREX1 Knockout IRF-Lucia Luciferase Reporter Cell Line
RAW-Lucia™ ISG-KO-TRIF CellsTRIF Knockout IRF-Lucia Luciferase Reporter Cell Line

THP-1 monocytes

THP1-Dual KO-cGAS CellsNEWHuman THP-1 Monocytes - cGAS knockout NF-κB-SEAP and IRF-Lucia Reporter Cells
THP1-Dual™ KO-IFI16 CellsHuman THP-1 Monocytes - IFI16 knockout NF-κB-SEAP and IRF-Lucia Reporter Cells
THP1-Dual KO-IFNAR2 CellsNEWHuman THP-1 Monocytes - IFNAR2 knockout NF-κB-SEAP and IRF-Lucia Reporter Cells
THP1-Dual™ KO-MyD CellsHuman THP-1 Monocytes - MyD88 knockout NF-κB-SEAP and IRF-Lucia Reporter Cells
THP1-Dual™ KO-STING CellsHuman THP-1 Monocytes - STING knockout NF-κB-SEAP and IRF-Lucia Reporter Cells
THP1-Dual™ KO-TREX1 CellsHuman THP-1 Monocytes - TREX1 knockout NF-κB-SEAP and IRF-Lucia Reporter Cells
Disclaimer: Our products are provided for research purpose only. Commercial applications may require licensing from third parties.
Note that the sequence of available ORFs provided by InvivoGen can differ from a given reference Genbank record due to genetic variations and/or alternative splicing. Customers should verify that the version of a gene sold by InvivoGen is suitable for the customer needs.
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