KO Cell Lines

InvivoGen provides a series of knockout (KO) cell lines generated by gene editing in popular cell lines, such as RAW 264.7 mouse macrophage and THP-1 human monocyte.

The genes targeted for KO are involved in the interferon (IFN) response to nucleic acids (e.g. double-stranded DNA or RNA) and to cyclic dinucleotides (CDNs). These genes include those encoding for the cytosolic DNA sensor cGAS, the cytosolic RNA sensor RIG-I, the cytosolic CDN sensor (and cGAS adaptor) STING, and the IFN signaling mediator TBK1.

Coupled with their parental cell lines, InvivoGen’s KO cell lines are utile for studying the function of these genes and for screening for molecules that activate pathways involving the proteins encoded by these genes.

The KO status of each cell line is thoroughly tested as follows:
  • Biallelic mutation/deletion confirmed by PCR and DNA sequencing;
  • Reporter activity validated by functional assays; and
  • KO stability guaranteed up to 20 passages.

KO Cell Lines

A549-Dual™ KO-RIG-I CellsNEW! RIG-I knockout NF-kB-SEAP & IRF-Lucia Reporter Cell Line

HEK-293 embryonic kidney cells

HEK-Blue™ ISG-KO-STING CellsNEW! STING Knockout IRF Reporter Cell Line

B16 melanoma

B16-Blue™ ISG-KO-STING Cells Murine B16 melanoma - STING Knockout IRF-reporter cells

RAW 264.7 macrophages

RAW-Lucia™ ISG-KO-cGAS Cells cGAS Knockout IRF-Lucia Luciferase Reporter Cell Line
RAW-Lucia™ ISG-KO-IFI16 Cells IFI16 Knockout IRF-Lucia Luciferase Reporter Cell Line
RAW-Lucia™ ISG-KO-IRF3 Cells IRF3 Knockout IRF-Lucia Luciferase Reporter Cell Line
RAW-Lucia™ ISG-KO-IRF7 Cells IRF7 Knockout IRF-Lucia Luciferase Reporter Cell Line
RAW-Lucia™ ISG-KO-MAVS CellsNEW! MAVS-Knockout IRF-Lucia Luciferase Reporter Cell Line
RAW-Lucia™ ISG-KO-MDA5 Cells MDA-5 Knockout IRF-Lucia Luciferase Reporter Cell Line
RAW-Lucia™ ISG-KO-RIG-I Cells RIG-I Knockout IRF-Lucia Luciferase Reporter Cell Line
RAW-Lucia™ ISG-KO-STING Cells STING Knockout IRF-Lucia Luciferase Reporter Cell Line
RAW-Lucia™ ISG-KO-TBK1 Cells TBK1 Knockout IRF-Lucia Luciferase Reporter Cell Line
RAW-Lucia™ ISG-KO-TREX1 Cells TREX1 Knockout IRF-Lucia Luciferase Reporter Cell Line
RAW-Lucia™ ISG-KO-TRIF Cells TRIF Knockout IRF-Lucia Luciferase Reporter Cell Line

THP-1 monocytes

THP1-Dual KO-cGAS CellsNEW! Human THP-1 Monocytes - cGAS knockout NF-κB-SEAP and IRF-Lucia Reporter Cells
THP1-Dual KO-IFI16 Cells Human THP-1 Monocytes - IFI16 knockout NF-κB-SEAP and IRF-Lucia Reporter Cells
THP1-Dual KO-IFNAR2 CellsNEW! Human THP-1 Monocytes - IFNAR2 knockout NF-κB-SEAP and IRF-Lucia Reporter Cells
THP1-Dual KO-MyD Cells Human THP-1 Monocytes - MyD88 knockout NF-κB-SEAP and IRF-Lucia Reporter Cells
THP1-Dual KO-STING Cells Human THP-1 Monocytes - STING knockout NF-κB-SEAP and IRF-Lucia Reporter Cells
THP1-Dual KO-TREX1 Cells Human THP-1 Monocytes - TREX1 knockout NF-κB-SEAP and IRF-Lucia Reporter Cells
Disclaimer: Our products are provided for research purpose only. Commercial applications may require licensing from third parties.
Note that the sequence of available ORFs provided by InvivoGen can differ from a given reference Genbank record due to genetic variations and/or alternative splicing. Customers should verify that the version of a gene sold by InvivoGen is suitable for the customer needs.
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