THP1-Lucia™ NF-κB Cells

THP1-Lucia™ cells were specifically designed for monitoring the NF-κB signal transduction pathway in a physiologically relevant cell line.

THP1-Lucia™ were derived from the human THP-1 monocyte cell line by stable integration of an NF-κB-inducible Luc reporter construct.
As a result, THP1-Lucia™ cells allow the monitoring of NF-κB activation by determining the activity of secreted luciferase (Lucia).
The levels of NF-κB-induced Lucia in the cell culture supernatant are readily assessed with QUANTI-Luc™, a luciferase detection reagent.

THP1-Lucia™ cells induce the activation of NF-κB in response to various PRR ligands.

THP1-Lucia™ cells are resistant to Zeocin™.

Figures for this product

NF-κB response of THP1-Lucia™ NF-κB cells


Antibiotic resistance: Zeocin™

Growth medium: RPMI 1640, 2 mM L-glutamine, 25 mM HEPES, 10% heat-inactivated fetal bovine serum, 100 μg/ml Normocin™, Pen-Strep (100 U/ml-100 μg/ml)

Guaranteed mycoplasma-free

Shipped on dry ice

These products are covered by a Limited Use License (See Terms and Conditions).


• 1 vial of THP1-Lucia™ NF-kB cells (5-7 x 106 cells) in Freezing Medium.
• 1 ml Normocin™ (50 mg/ml).
• 100 μl Zeocin™ (100 mg/ml).
• 1 pouch of QUANTI-Luc™.
• 10e9 cells HKLM (Heat killed Listeria monocytogenes; positive control of TLR2 activity).


THP1-Lucia™ were derived from the human THP-1 monocyte cell line by stable integration of an NF-κB-inducible Luc reporter construct. 
THP1-Lucia™ cells feature the Lucia gene, a secreted luciferase reporter gene, driven by an IFN-β minimal promoter fused to five copies of the NF-κB consensus transcriptional response element and three copies of the c-Rel binding site.
As a result, THP1-Lucia™ cells allow the monitoring of NF-κB activation by determining the activity of Lucia luciferase.


Recent articles using InvivoGen THP1-Lucia™ NF-κB Cells


THP1-Lucia™ NF-κB Cells

Description NF-κB Luc Reporter Monocytes
Cat. Codethpl-nfkb
Unit Size3-7 x 10e6 cells
Price For price or distributor address,
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Note that the sequence of available ORFs provided by InvivoGen can differ from a given reference Genbank record due to genetic variations and/or alternative splicing. Customers should verify that the version of a gene sold by InvivoGen is suitable for the customer needs.
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