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Coding sequence in expression plasmid

E. coli Cytosine Deaminase fused to Uracil Phosphoribosyltransferase

Cytosine deaminase (CD) is an enzyme found in prokaryotes and lower eukaryotes but absent in higher eukaryotes. It converts cytosine and its analog, 5-fluorocytosine (5-FC), to uracil and 5-fluorouracil (5-FU) respectively. 5-FU is a toxic compound commonly used as anticancer drug.
To further increase the efficiency of the CD/5-FC pair, InvivoGen has fused the gene encoding uracil phosphoribosyl transferase to the CD gene. UPRT converts 5-FU to 5-FUMP, an irreversible inhibitor of thymidylate synthase.

This gene is available in pUNO1 expression plasmid selectable with Blasticidin.


Bacteria codA::upp (pUNO1-codAupp)

   ORF Size: 1935 bp
   Subclone: NcoI - NheI


- 20 µg of lyophilized DNA.
- 4 pouches of E. coli Fast-Media® Blas (2 TB and 2 Agar)
- 1 ml blasticidin at 10 mg/ml



Description pUNO1 bearing the bacterial codA::upp fusion gene
Cat. Codepuno1-codaupp
Unit Size20 µg
Price For price or distributor address,
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Disclaimer: Our products are provided for research purpose only. Commercial applications may require licensing from third parties.
Note that the sequence of available ORFs provided by InvivoGen can differ from a given reference Genbank record due to genetic variations and/or alternative splicing. Customers should verify that the version of a gene sold by InvivoGen is suitable for the customer needs.
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