pDRIVE-TRP1
| Product | Unit size | Cat. code | Docs. | Qty. | Price | |
|---|---|---|---|---|---|---|
|
pDRIVE-mTRP pDRIVE bearing mouse TRP1 promoter - LacZ |
Show product |
20 µg |
pdrive-mtrp
|
|
||
|
pDRIVE5SEAP-mTRP pDRIVE bearing mouse TRP1 promoter - SEAP |
Show product |
20 µg |
pdrive5s-mtrp
|
|
||
|
pDRIVE5Lucia-mTRP pDRIVE bearing mouse TRP1 promoter - Lucia |
Show product |
20 µg |
pdrive5lc-mtrp
|
|
Tyrosinase-related protein 1 (TRP1) promoter in pDRIVE expression plasmid
The TRP1 gene encodes the tyrosinase-related protein 1, an enzyme involved in the process that converts tyrosinase to melanin pigments.
Expression of TRP1 is restricted to melanocytes and melanoma cells partly as a consequence of transcriptional regulation of its mRNA.
The TRP1 promoter shares with the tyrosinase promoter an 11-bp motif termed the M box located upstream of the TATA box. This M box plays a key role in the regulation of the TRP1 expression by cyclic AMP (cAMP) [1].
Tissue-specific expression of transgenes, such as the IL-2 and HSV1-tk genes, driven by the TRP1 promoter has been demonstrated in murine model systems, achieving marked antitumor effects [2].
References:
1. Bertolotto C. et al. 1996. Different cis-acting elements are involved in the regulation of TRP1 and TRP2 promoter activities by cyclic AMP: pivotal role of M boxes (GTCATGTGCT) and of microphthalmia Mol Cell Biol. 18(2):694-702.
2. Hart IR. 1996. Tissue-specific promoters in targeting systemically delivered gene therapy. Semin Oncol. 23(1):154-8.
Specifications
pDRIVE bearing mouse TRP1 promoter - LacZ, SEAP or Lucia
Gene Promoter: Tyrosinase Related Protein
Specificity: Melanocytes & melanoma
Backbone: pDRIVE, pDRIVE5-SEAP or pDRIVE5-Lucia
Selection: Zeocin™
pDRIVE contents
- 20 µg of lyophilized DNA
- 1 ml of Zeocin™ (100 mg/ml)
Product is shipped at room temperature
