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pDRIVE-GRP94

Product Unit size Cat. code Docs. Qty. Price

pDRIVE-hGRP94

pDRIVE bearing human GRP94 promoter - LacZ

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20 µg

pdrive-hgrp94
+-
$554

pDRIVE5SEAP-hGRP94

pDRIVE bearing human GRP94 promoter - SEAP

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20 µg

pdrive5s-hgrp94
+-
$554

pDRIVE5Lucia-hGRP94

pDRIVE bearing human GRP94 promoter - Lucia

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20 µg

pdrive5lc-hgrp94
+-
$554

pDRIVE-mGRP94

pDRIVE bearing mouse GRP94 promoter - LacZ

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20 µg

pdrive-mgrp94
+-
$554

pDRIVE5SEAP-mGRP94

pDRIVE bearing mouse GRP94 promoter - SEAP

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20 µg

pdrive5s-mgrp94
+-
$554

pDRIVE5Lucia-mGRP94

pDRIVE bearing mouse GRP94 promoter - Lucia

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20 µg

pdrive5lc-mgrp94
+-
$554

pDRIVE-rGRP94

pDRIVE bearing rat GRP94 promoter - LacZ

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20 µg

pdrive-rgrp94
+-
$554

pDRIVE5SEAP-rGRP94

pDRIVE bearing rat GRP94 promoter - SEAP

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20 µg

pdrive5s-rgrp94
+-
$554

pDRIVE5Lucia-rGRP94

pDRIVE bearing rat GRP94 promoter - Lucia

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20 µg

pdrive5lc-rgrp94
+-
$554

GRP94 promoter (Ubiquitous, stress-inducible) in pDRIVE expression plasmid

Stress inducible

The glucose-regulated proteins, GRP78 and GRP94, function as molecular chaperones. They are expressed constitutively in most cell types under normal growth conditions and are highly induced in stressed cells.

The genes for GRP78 and GRP94 are coordinately regulated at the transcriptional level under a variety of stress conditions. Inducing factors are cellular environments of low glucose or oxygen and reagents that disrupt the ER function such as calcium ionophores.

The GRP78 and GRP94 promoters are highly conserved and share a common regulatory domain [1]. The GRP78 promoter is known to retain its strong activity in differentiated and undifferentiated tissues whereas GRP94 is mainly effective in differentiated cells [2].

Furthermore, the GRP78 promoter can increase the expression levels of HSV-tk inside tumors resulting in complete eradication of tumor mass, with no recurrence of tumor growth [3].

 

References:

1. Chang SC. et al. 1989. Glucose-regulated protein (GRP94 and GRP78) genes share common regulatory domains and are coordinately regulated by common trans-acting factors. Mol Cell Biol 9:2153-62.
2. Kim SL. et al. 1990. Expression of the glucose-regulated proteins (GRP94 and GRP78) in differentiated and undifferentiated mouse embryonic cells and the use of the GRP78 promoter as an expression system in embryonic cells. Differentiation 42:153-9.
3. Gazit G. et al. 1999. Use of the glucose starvation-inducible glucose-regulated protein 78 promoter in suicide gene therapy of murine fibrosarcoma. Cancer Res 59: 3100-6.

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Specifications

pDRIVE bearing human GRP94 promoter - LacZ, SEAP or Lucia

Gene Promoter: Glucose-Regulated Protein 94

Specificity: Ubiquitous, stress inducible

Backbone: pDRIVE, pDRIVE5-SEAP or pDRIVE5-Lucia

Selection: Zeocin™

pDRIVE bearing mouse GRP94 promoter - LacZ, SEAP or Lucia

Gene: Glucose-Regulated Protein 94

Specificity: Ubiquitous, stress inducible

Backbone: pDRIVE, pDRIVE5-SEAP or pDRIVE5-Lucia

Selection: Zeocin™

pDRIVE bearing rat GRP94 promoter - LacZ, SEAP or Lucia

Gene: Glucose-Regulated Protein 94

Specificity: Ubiquitous, stress inducible

Backbone: pDRIVE, pDRIVE5-SEAP or pDRIVE5-Lucia

Selection: Zeocin™

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pDRIVE contents

  • 20 µg of lyophilized DNA
  • 1 ml of Zeocin™ (100 mg/ml)

room temperature Product is shipped at room temperature

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Details

pDRIVE map

pDRIVE5-SEAP map

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Citations

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