Plasmocin™

Mycoplasma Removal Agent

Plasmocin™ is used to cure cell lines infected by mycoplasma and related cell wall-less bacteria.
PlasmocinPlasmocin™ can also be used as a routine addition in liquid media to prevent mycoplasma and more generally bacterial contamination in small and large animal cell cultures.
More info on Mycoplasma eradication

Plasmocin™ is a well-established antimycoplasma reagent. It contains two bactericidal components strongly active against mycoplasmas that allow their elimination in only 2 weeks. 
The first component acts on the protein synthesis machinery while the second acts on the DNA replication. These two specific and separate targets are found only in mycoplasmas and many other bacteria and are completely absent in eukaryotic cells.
 
Plasmocin™ is also active at low concentrations on a broad range of Gram positive and Gram negative bacteria that are otherwise resistant to the mixture of streptomycin and penicillin, and exhibits no toxicity in eukaryotic cells.
 
Many cell lines infected by mycoplasmas have been successfully treated with Plasmocin™ [1], including primary cancer cell lines [2], virus producing cells [3] and induced pluripotent stem cells [4], without inducing any permanent alterations. Further, in a recent study, a 2 week treatment with Plasmocin was found to effectively cure mycoplasma contamination in human embryonic stem cells without affecting their stemness and pluripotency nor cell viability [5].

Warning: InvivoGen's anti-mycoplasma products are suitable for research purposes only, and not for human or animal care.

Specifications

 
• Plasmocin Prophylactic (2.5 mg/ml): to prevent mycoplasma contamination, use at 2.5 - 5 µg/ml on a regular basis
 
• Plasmocin Treatment (25 mg/ml): to eliminate mycoplasmas, use at 25 µg/ml for 2 weeks

Treat up to 25 cell lines in T75

Contents

Plasmocin™ is provided as a yellow solution at different concentrations:

-  25 mg/ml (Plasmocin™Treatment)

-  2.5 mg/ml (Plasmocin™ Prophylactic)

Description

In contrast to other anti-mycoplasma compounds, Plasmocin™ is active on both free mycoplasmas and intracellular forms. This advantage is conferred by one component of Plasmocin™ which is actively transported into mammalian cells. It ensures that following treatment with Plasmocin™ a cell culture is not reinfected by mycoplasmas released from intracellular compartments of infected cells.

In all animal cell lines tested to date, even at five times the working concentration, no apparent adverse effect on cellular metabolism is observed.

No resistance in liquid cultures of mycoplasmas has ever been identified in repeated experiments attempting to measure the mutation rate. Therefore, development of resistant mycoplasma strains is virtually eliminated.

Plasmocin™ is also active at low concentrations on a broad range of Gram positive and Gram negative bacteria that are otherwise resistant to the mixture of streptomycin and penicillin, and exhibits no toxicity in eukaryotic cells.

Many cell lines infected by mycoplasmas have been successfully treated with Plasmocin™, including embryonic stem cells, hybridomas and retrovirus packaging cells.

Comparison of the most common anti-mycoplasma agents[1-3]

ProductSupplierTreatmentEase of useEfficacyCytotoxicityResistance
BM-Cyclin Roche 3 weeks - +++ + +/-
Ciprobay Bayer 12 to 20 days + ++ +/- +
MRA ICN 1 to 2 weeks + ++ +/- +
Plasmocin InvivoGen 2 weeks + +++ +/- -

Antibiotics commonly used in cell culture are inactive on mycoplasma (e.g. penicillins and streptomycin). Three classes of antibiotics have been shown to kill mycoplasma at relatively low concentrations: tetracyclines, macrolides and quinolones. Tetracyclines and macrolides block the protein synthesis by interfering with ribosome translation, while quinolones inhibit the replication of bacterial DNA.
Several antibiotics are commercially available for the removal of mycoplasma: BM-cyclin (Roche) contains a macrolide and a tetracycline, Ciprobay (Bayer, available only with a prescription) and MRA (ICN) are both quinolones. Plasmocin™ is the only antimycoplasma reagent that combines a macrolide and a quinolone. Unlike BM-Cyclin that requires the sequential and cyclic use of 2 antibiotics, Plasmocin™ is ready-to-use and can be added to the culture medium directly. Furthermore, the 2 antibiotics in Plasmocin™ act on separate targets blocking protein synthesis and DNA replication, whereas the 2 antibiotics in BM-Cyclin are both inhibitors of protein synthesis. Therefore, Plasmocin™ is more effective in removing mycoplasma and prevents the appearance of resistant strains. In contrast to other anti-mycoplasma compounds, Plasmocin™ is active on both free mycoplasma as well as intracellular forms. This advantage is conferred by one component of Plasmocin™ which is actively transported into mammalian cells. It ensures that following treatment with Plasmocin™ a cell culture is not reinfected by mycoplasma released from intracellular compartments of infected cells. To date, no consistent and permanent alterations that affect the eukaryotic cells during and after the treatment have been detected[1].

1. Uphoff CC, Drexler HG., 2005. Eradication of mycoplasma contaminations. Methods Mol Biol. 290:25-34.
2. Somasundaram C. et al., 1992. Use of ciprofloxacin and BM-Cyclin in mycoplasma decontamination.In Vitro Cell Dev Biol. 28A(11-12):708-10
3. Drexler HG. et al., 1994. Treatment of mycoplasma contamination in a large panel of cell cultures. In vitro Cell Dev Biol Anim. 30A(5):344-7

Ref.

1. Uphoff CC et al., 2012. Treatment of mycoplasma contamination in cell cultures with Plasmocin. J Biomed Biotechnol. 2012:267678.
2. Rongvaux A et al., 2014. Development and function of human innate immune cells in a humanized mouse model. Nat Biotechnol. [Epub ahead of print]
3. Baronti C. et al., 2013. Mycoplasma removal: simple curative methods for viral supernatants. J Virol Methods. 187(2):234-7
4. Deng F. et al. 2012. Generation of induced pluripotent stem cells from human Tenon's capsule fibroblasts. Mol Vis. 18:2871-81
5. Romorini L. et al, 2013. Effect of antibiotics against Mycoplasma sp. on human embryonic stem cells undifferentiated status, pluripotency, cell viability and growth. PLoS One. 8(7):e70267

Citations

Recent articles using InvivoGen Plasmocin™

ORDERING

Plasmocin™ prophylactic

Description Removal agent to prevent mycoplasma contamination
Cat. Code ant-mpp
Unit Size 25 mg (10 x 1 ml)
Price For price or distributor address,
please select your country

Plasmocin™ treatment

Description Removal agent to eliminate mycoplasmas
Cat. Code ant-mpt
Unit Size 50 mg (2 x 1 ml)
Price For price or distributor address,
please select your country
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