Anti-hSTING-IgG

Monoclonal antibody against human STING

Anti-hSTING-IgG is a monoclonal mouse IgG1 antibody against human STING (hSTING).

STING (stimulator of interferon genes) is essential for the interferon (IFN) response to cytoplasmic foreign or self-DNA. It directly senses cyclic dinucleotides (CDNs), which are important messengers in bacteria and innate immune agonists in mammals [1]. Several STING variants, that differ in their responses to CDNs, have been described in the human population [2].

Anti-hSTING-IgG antibody was generated by InvivoGen using DNA vaccination and screened for its ability to bind hSTING using ELISA. This antibody recognizes the most prevalent isoform “wild‑type” R232 and HAQ hSTING variants. No reactivity was observed with the STING‑knockout cells.

Anti-hSTING-IgG is produced in hybridomas and purified by affinity chromatography with protein G. It can be used to detect human STING using ELISA and Western blot.

References:

1. Sun L. et al., 2013. Cyclic GMP-AMP synthase is a cytosolic DNA sensor that activates the type I interferon pathway. Science. 339:786-91. 
2. Yi G. et al., 2013. Single nucleotide polymorphisms of human STING can affect innate immune response to cyclic dinucleotides. PLOS One. 8:e77846. 

Figures

Anti-hSTING-IgG recognition of recombinant hSTING. Recombinant hSTING was immobilized at 1 μg/mL (50 μL/well) in 96-well plate, followed by the addition of Anti-hSTING capture antibody and HRP-conjugated secondary antibody. Absorbance was read at 450 nm following addition of HRP substrate.

Detection of human STING by Western blot. Western blot analysis of lysates (40 μg) using Anti‑hSTING‑IgG (0.2 μg/ml), followed by HRP‑conjugated goat anti-mouse IgG1 secondary antibody (1:3000 dilution).
A: THP1‑Dual™ KO‑STING cells
B: THP1-Dual™ cells (endogenously expresses the HAQ hSTING variant)
C: 293T-Dual™ Null cells (does not express functional hSTING protein)
D: 293T-Dual™ hSTING-R232 cells (stably transfected with the R232 hSTING variant)

Specifications

Clonality: Monoclonal antibody.

Target: Human STING (hSTING) R232 variant (soluble CDN-binding domain).

Specificity: Reacts with the “wild‑type” R232 (R71‑G230-R232-R293) and HAQ (H71‑A230-R232-Q293) hSTING variants. This antibody reacts very poorly with murine STING.

Isotype: Mouse IgG1.

Source: Hybridoma.

Clone: 3B5.

Purity: Purified by affinity chromatography with protein G.

Quality control:
   - The antibody has been validated by Western blot.
   - The absence of bacterial contamination (e.g. lipoproteins and endotoxins) has been confirmed using HEK-Blue™ TLR2 and HEK-Blue™ TLR4 cells.

Contents

100 µg purified anti-hSTING-IgG provided azide-free and lyophilized.

Product is shipped at room temperature.

Store lyophilized antibody at -20°C.

Lyophilized product is stable for at least 1 year.