Anti-hSTING-IgG1
Anti-hSTING-IgG | Unit size | Cat. code | Docs | Qty | Price |
---|---|---|---|---|---|
Monoclonal antibody against human STING |
100 µg |
mabg-hstg |
Monoclonal antibody against human STING
Anti-hSTING-IgG is a monoclonal mouse IgG1 antibody against human STING (hSTING).
STING (stimulator of interferon genes) is essential for the interferon (IFN) response to cytoplasmic foreign or self-DNA. It directly senses cyclic dinucleotides (CDNs), which are important messengers in bacteria and innate immune agonists in mammals [1]. Several STING variants, that differ in their responses to CDNs, have been described in the human population [2].
Anti-hSTING-IgG1 antibody was generated by InvivoGen using DNA vaccination and screened for its ability to bind hSTING using ELISA. This antibody recognizes the most prevalent isoform “wild‑type” R232 and HAQ hSTING variants. No reactivity was observed with the STING‑knockout cells.
Anti-hSTING-IgG1 is produced in hybridomas and purified by affinity chromatography with protein G. It can be used to detect human STING using ELISA and Western blot.
References:
1. Sun L. et al., 2013. Cyclic GMP-AMP synthase is a cytosolic DNA sensor that activates the type I interferon pathway. Science. 339:786-91.
2. Yi G. et al., 2013. Single nucleotide polymorphisms of human STING can affect innate immune response to cyclic dinucleotides. PLOS One. 8:e77846.
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Specifications
Clonality: Monoclonal antibody
Target: Human STING (hSTING) R232 variant (soluble CDN-binding domain)
Specificity: Reacts with the “wild‑type” R232 (R71‑G230-R232-R293) and HAQ (H71‑A230-R232-Q293) hSTING variants. This antibody reacts very poorly with murine STING.
Clone: 3B5
Isotype: Mouse IgG1, kappa
Control: Mouse Control IgG1
Source: Hybridoma cells
Purity: Purified by affinity chromatography with protein G
Tested application: Detection antibody for western blot and ELISA. It can also be used for flow cytometry
Quality control:
- The antibody has been validated by Western blot.
- The absence of bacterial contamination (e.g. lipoproteins and endotoxins) has been confirmed using HEK-Blue™ TLR2 and HEK-Blue™ TLR4 cells.
Contents
100 µg purified anti-hSTING-IgG provided azide-free and lyophilized.
Product is shipped at room temperature.
Store lyophilized antibody at -20°C.
Lyophilized product is stable for at least 1 year.